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Method for quickly producing acetic acid by performing submerged fermentation to cellulose

A technology of deep fermentation and cellulose, applied in the field of bioengineering, can solve the problems of polluting the environment, waste of renewable resources, etc., and achieve the effects of solving the pollution problem, high yield of acetic acid products, and wide sources.

Active Publication Date: 2012-10-03
JINAN GUOLI BIOLOGICAL SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The residues of corn cobs after producing xylose, furfural, xylo-oligosaccharides and other products account for about 60% of the total amount of corn cobs. At present, these waste residues are burned or thrown away as waste, which not only wastes a lot of renewable resources, but also polluted the environment

Method used

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  • Method for quickly producing acetic acid by performing submerged fermentation to cellulose

Examples

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Comparison scheme
Effect test

Embodiment 1

[0023] Get the cellulose containing 65 parts by weight, the lignin of 25 parts by weight, and the xylose residue of 12 parts by weight as raw materials, proceed as follows:

[0024] a. Put the raw material of industrial xylose waste residue into the steam explosion machine, and pretreat it for 150s under the condition of 2Mpa;

[0025] b. Take the pretreated industrial xylose waste residue raw material in a step according to the raw material: water=1:1 weight ratio, add water into the refiner for refining, control the refining weight concentration to be 20%, and the refining particle size is 80 mesh , and then placed in a saccharification tank, under the conditions of temperature 45°C and pH=6, the raw material ratio of 1500 units / g industrial xylose waste residue was obtained by exo-β-glucanase and endo-β-glucanase. Carbohydrase and β-glucosidase in any combination of cellulase, added in 4 times with an interval of 6 hours, enzymatic hydrolysis for 28 hours, and then filtered...

Embodiment 2

[0028] Get the cellulose containing 55 parts by weight, the lignin of 30 parts by weight, and the xylose residue of 6 parts by weight as raw materials, proceed as follows:

[0029] a. Put the raw material of industrial xylose waste residue into the steam explosion machine, and pretreat it for 90s under the condition of 3Mpa;

[0030] b. Take the pretreated industrial xylose waste residue raw material in a step according to the raw material: water=1:10 parts by weight, add water into the refiner for refining, control the refining weight concentration to be 10%, and the refining particle size is 40 mesh , and then placed in a saccharification tank, under the conditions of temperature 60°C and pH=4, the raw materials of exo-β-glucanase and endo-β-glucan were obtained according to the ratio of 500 units / g of industrial xylose waste residue. Carbohydrase and β-glucosidase in any combination of cellulase, added in 2 times with an interval of 10 hours, enzymatic hydrolysis for 20 hou...

Embodiment 3

[0033] Get the cellulose that contains 58 weight parts, the lignin of 28 weight parts, the xylose residue of 10 weight parts as raw material, carry out as follows:

[0034] a. Put the raw material of industrial xylose waste residue into the steam explosion machine, and pretreat it for 100s under the condition of 2.5Mpa;

[0035] b. Take the pretreated industrial xylose waste residue raw material in a step according to the raw material: water=1:7 weight ratio, add water into the refiner for refining, control the refining weight concentration to be 13%, and the refining particle size is 70 mesh , and then placed in a saccharification tank, under the conditions of temperature 50°C and pH=5, the raw materials of exo-β-glucanase and endo-β-glucan were obtained according to the ratio of 900 units / g of industrial xylose waste residue. Carbohydrase and β-glucosidase in any combination of cellulase, added in 3 times with an interval of 7 hours, enzymatic hydrolysis for 25 hours, and th...

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Abstract

The invention provides a method for quickly producing acetic acid by performing submerged fermentation to cellulose; 55-65 parts of the cellulose, 25-33 parts of lignin and 2-12 parts of industrial xylose waste residue are used as raw materials (part by weight); the method comprises the following steps: a, adding the industrial xylose waste residue raw material to a steam blasting machine and treating the industrial xylose waste residue raw material at 2-3MPa; b, adding water to the raw material pre-treated in the step a according to proportion of raw material to water to be 1: 1-10; grinding in a grinding machine to obtain slurry, placing in a saccharification tank, adding cellulase, and performing enzymolysis so as to obtain saccharified liquid; and c, adding active dried yeast and acetic bacteria to the saccharified liquid of the step b, performing fermentation simultaneously, and dewatering so as to obtain a product.

Description

technical field [0001] The invention relates to a method for producing acetic acid, which belongs to the field of bioengineering, and specifically relates to a rapid production method using xylose residue, furfural residue, xylooligosaccharide production residue, straw, wheat straw and other cellulose-rich materials as raw materials. acetic acid method. Background technique [0002] Acetic acid is an important chemical intermediate and chemical reaction solvent, from which hundreds of downstream products can be derived, and is widely used in medicine, synthetic fibers, light industry, textiles, leather, pesticides, explosives, rubber, metal processing, food As well as various industrial fields such as the synthesis of fine organic chemicals, it is an important basic organic chemical raw material that has developed rapidly in the world in recent years. Since 1999, the annual growth rate of the demand for acetic acid in the world has been between 3% and 5%, and the fastest gr...

Claims

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Application Information

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IPC IPC(8): C12P7/54C12P7/10C12P19/14C12R1/02C12R1/865
CPCY02E50/16Y02E50/10
Inventor 高艳华袁博任明
Owner JINAN GUOLI BIOLOGICAL SCI & TECH
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