Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Anti-herbicide gene expression cassette, expression vector with the same expression cassette, and application thereof for culturing anti-herbicide plants

A technology of herbicide-resistant genes and expression vectors, applied in applications, plant products, genetic engineering, etc., can solve the problems of different herbicides, the impact of herbicide spraying doses on crop production, and the death of transgenic herbicide-resistant crops, etc. The effect of low level, improvement of production capacity, and expansion of sensitivity

Inactive Publication Date: 2012-10-10
林忠平
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the process of using glyphosate, it is found that different weeds have different resistance to herbicides. Some weeds can survive when the amount of herbicide sprayed is insufficient, but too large a dose of herbicide will also affect it to a certain extent. The production of crops, and even caused the death of some genetically modified herbicide-resistant crops

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anti-herbicide gene expression cassette, expression vector with the same expression cassette, and application thereof for culturing anti-herbicide plants
  • Anti-herbicide gene expression cassette, expression vector with the same expression cassette, and application thereof for culturing anti-herbicide plants
  • Anti-herbicide gene expression cassette, expression vector with the same expression cassette, and application thereof for culturing anti-herbicide plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Construction of Sprout-Specific Expressing cp4EPSPS Gene Expression Cassette

[0030] The EPSPS gene was artificially synthesized according to the plant preferred codons by using molecular biology methods, named as cp4EPSPS gene, and its base sequence is shown in SEQ ID NO: 2;

[0031] The tobacco leafy gene promoter was cloned by molecular biology methods, and its base sequence is shown in SEQ ID NO: 2;

[0032] Construct the expression cassette of the shoot specific expression cp4EPSPS gene, its structure is as follows figure 1 As shown, the expression cassette is composed of leafy gene promoter, cp4EPSPS gene and T-nos terminator.

Embodiment 2

[0033] Example 2 constructs an expression vector comprising two cp4EPSPS gene expression cassettes

[0034] see figure 2 , the expression vector p3300-leafy-CP4EPSPS-35s-CP4EPSPS contains two cp4EPSPS gene expression cassettes, one of which is composed of leafy gene promoter, cp4EPSPS gene and T-nos terminator, and the other expression cassette is composed of 35S promoter, cp4EPSPS gene and T-nos terminator, the expression vector is constructed on the basis of commercially available p3300 plasmid.

Embodiment 3

[0035] Example 3 Using Agrobacterium-mediated Transformation to Obtain Transplanted Shoots Specific Expression of cp4EPSPS Gene Tobacco

[0036] The plant expression vector p3300-leafy-CP4EPSPS-35s-CP4EPSPS was transformed into Agrobacterium LBA 4404, and then tobacco was transformed by Agrobacterium-mediated leaf disc method.

[0037] Tobacco calluses after Agrobacterium infection were differentiated into seedlings, and PPT-resistant plants were obtained through PPT primary screening, and some plants were transplanted in the greenhouse. After 40 days, genomic DNA was extracted for detection. Genomic DNA was used as a template. The PCR detection results are shown in image 3 , is the detection electropherogram of some plants transfected with CP4EPSPS gene.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a genetic engineering field, and provides an anti-herbicide gene expression cassette. The anti-herbicide gene expression cassette, being specifically expressed at shoot parts of plants, comprises a leafy gene promoter, an anti-herbicide gene, and a terminator. As a cp4EPSPS gene is expressed specifically at the shoot parts of transgenic plants, the damage degree of the transgenic plants is significantly reduced after glyphosate is sprayed. Due to the sensitivity difference between the transgenic plants and weeds to the herbicide being enlarged, when the herbicide is used, the damage degree of the transgenic plants is reduced to a minimum and the crop productivity is improved.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to a herbicide-resistant gene expression cassette capable of enhancing the herbicide-resistant characteristics of young shoots, an expression vector with the expression cassette and its application in cultivating herbicide-resistant plants. Background technique [0002] At present, the most commonly used herbicide is glyphosate, which is a non-selective herbicide, usually used at 2.0-5.0kg a.i. / hm2. It was successfully developed by Monsanto in 1971 and is the best-selling herbicide in the world. , and its mechanism of action is mainly to competitively inhibit the activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in the shikimate pathway. [0003] In 1996, the United States first began to plant glyphosate-resistant transgenic soybeans. In 2008, the planting area of ​​herbicide-resistant transgenic crops in the world was 79 million hectares, accounting for 69% of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/84A01H5/00
Inventor 林忠平杜娟胡鸢雷徐志超连肖华
Owner 林忠平
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com