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Use of effective part of traditional Chinese medicine in HIV latency-resistant treatment

A technology of effective parts and latent infection, applied in the field of medicine, can solve the problems of high toxicity and side effects, unsatisfactory, etc.

Active Publication Date: 2012-10-17
SHANGHAI XINHAO BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this strategy has several treatment options in clinical practice, the results are still unsatisfactory. Either the activator is ineffective, or it is effective but has severe side effects.

Method used

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  • Use of effective part of traditional Chinese medicine in HIV latency-resistant treatment
  • Use of effective part of traditional Chinese medicine in HIV latency-resistant treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Preparation of effective anti-HIV fraction of Atractylodes macrocephala

[0072] The traditional Chinese medicine Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizome (1kg, chopped medicinal material) was reflux extracted with 95% ethanol (8L x 3), and the ethanol was recovered to obtain an ethanol extract (0.19kg).

[0073] The ethanol extract was dissolved in 200mL hot water, and after cooling, it was sequentially extracted with petroleum ether (200mL x 3) and dichloromethane (200mL x 3) to obtain a dichloromethane extract (21g).

[0074] The dichloromethane extract was subjected to silica gel column chromatography (1kg, column diameter: 10cm), eluted with petroleum ether: ethyl acetate (100:1) (10L) to remove the fat part, and then washed with petroleum ether: ethyl acetate (1: 1) (10L) was eluted to obtain the effective fraction (9.1g).

[0075] The ob...

Embodiment 2

[0082] Effects of effective fractions on HIV latency-induced activation

[0083] 1. Method

[0084] C11 cell is a latently infected cell model of HIV, which is obtained by infecting the human T lymphocyte cell line Jurkat with HIV-1 lentivirus carrying the EGFP reporter gene, through cell sorting and HIV integration detection, and has HIV integration but no expression EGFP T lymphocyte line. C11 cells are Jurkat stable strains that are infected by the HIV lentivirus carrying the reporter gene GFP and do not express at the same time, so they are used for screening and activating HIV-1 drugs for latent infection (see application number 200810038851.X, invention title "a screening for activating latent infection HIV-1 compound T lymphocytes and its preparation method" Chinese patent application), the preservation number is CCTCCNO.C200821.

[0085] In this example, 2×10 per hole 4 C11 cells were planted in a 96-well plate, and 100 μl of 1640 medium (Gibco) containing 10% FBS (...

Embodiment 3

[0090] Effects of Different Concentrations of Effective Fractions on HIV Latency Induction and Activation

[0091] Repeat Example 2, the only difference is that the effective fraction of Atractylodes Rhizome with a final concentration of 0.2 μg / mL, 0.5 μg / mL, 2 μg / mL, or 5 μg / mL is used to replace the effective fraction of Atractylodes Rhizome with a final concentration of 1 μg / mL.

[0092] The results showed that, at the above concentrations, the proportion of green fluorescent positive cells in HIV latently infected cells treated with different active parts of Atractylodes macrocephala was 10-80%, much higher than the background activation of less than 1% in the control. This shows that the effective part of the present invention has a significant intervention effect on HIV latently infected cells.

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Abstract

The invention provides a use of an effective part of a traditional Chinese medicine in HIV latency-resistant treatment. Through an ingenious activity screening method, it is found that a traditional Chinese medicine rhizoma atractylodis macrocephalae has effects of resisting AIDS. Through a solvent extraction method, a gradient extraction method, a silica gel column chromatography and the combination with activity screening, an effective part of rhizoma atractylodis macrocephalae is determined and is prepared. The prepared effective part of rhizoma atractylodis macrocephalae can be used as an active component for resisting AIDS. The effective part of rhizoma atractylodis macrocephalae has an effect of HIV latency intervention, can be combined with a drug for resisting retroviruses, can accelerate the removal of latent virus reservoirs, and provides a novel approach for thorough curing of AIDS.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the discovery of an effective part of an active traditional Chinese medicine and its preparation method, as well as its application in anti-HIV latent treatment. Background technique [0002] Acquired Immunodeficiency Syndrome (AIDS) is an infectious disease caused by HIV infection that seriously endangers people's life and health. According to WHO statistics, there are more than 40 million AIDS patients in the world, with 5 million new patients and 3 million deaths each year. [0003] At present, the clinical treatment of AIDS is mainly Highly active antiretroviral therapy (HAART), which not only effectively controls HIV replication, but also can rebuild the immune function of AIDS patients, opening the door of hope for the treatment of AIDS . [0004] People once hoped to completely eliminate HIV in the body by virtue of HAART, so as to achieve the goal of completely curing AIDS. Howev...

Claims

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Application Information

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IPC IPC(8): A61K36/284C12N5/07C12N7/00A23L1/29A61P31/18A61K125/00A61K127/00A61K135/00A23L33/00
Inventor 朱焕章马忠俊
Owner SHANGHAI XINHAO BIOLOGICAL TECH