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Streptomyces hygroscopicus Sh-43 strain, its fermentation solution production method and application

A technology of Streptomyces hygroscopicus and sh-43, which is applied in the field of agricultural microorganisms to achieve stable antagonistic effects, simple culture conditions, and good control effects

Inactive Publication Date: 2012-10-17
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is aimed at the lack of rice varieties resistant to sheath blight, and rice sheath blight bacteria have developed resistance to fungicides used in production, etc.

Method used

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  • Streptomyces hygroscopicus Sh-43 strain, its fermentation solution production method and application
  • Streptomyces hygroscopicus Sh-43 strain, its fermentation solution production method and application
  • Streptomyces hygroscopicus Sh-43 strain, its fermentation solution production method and application

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Embodiment 1

[0034] Example 1 Isolation, purification and screening of Streptomyces hygroscopicus Sh-43 bacterial strain

[0035] 1.1 Medium

[0036] (1) PDA medium: 200 g potato, 20 g glucose, 18 g agar powder, 1000 mL water, natural pH.

[0037] (2) Gao's I medium: 20 g soluble starch, KNO 3 1 g, NaCl 0.5 g, K 2 HPO 4 0.5 g, MgSO 4 0.5 g, FeSO 4 0.01 g, 20 g agar, 1000 mL water, pH 7.2~7.4.

[0038] (3) Seed medium: 60 g soluble starch, 20 g yeast powder, KH 2 P0 4 0.5 g, NaCl 1.5 g, MgSO 4 0.5 g, FeS0 4 0.01 g, water 1000 mL, pH 7.2.

[0039] (4) Fermentation medium: 20 g soluble starch, 1 g yeast powder, 0.5 g NaCl, K 2 HPO 4 0.5 g, MgSO 4 0.5 g, FeSO 4 0.01 g, 1000 mL of water, pH 7.2~7.4.

[0040] Isolation and purification of actinomycetes strains

[0041]The Streptomyces hygroscopicus Sh-43 strain was isolated from the soil samples of the planting site of Platanus erculus in the suburbs of Jinhua City, Zhejiang Province. The soil samples were air-dr...

Embodiment 2

[0049] Embodiment 2 The technological process of producing Sh-43 bacterial strain fermented liquid by fermentation method

[0050] Under sterile conditions, use an inoculation needle to pick a little Streptomyces hygroscopicus Sh-43 strain in test tubes to preserve the strains, transfer them to the No. 1 culture plate of Gao Shi, and activate and cultivate them at 30 °C for 168 h. In the same way, the activated strains were inserted into the Gaussian No. 1 culture plate, and cultivated at 30 °C for 120 h, as the primary seeds. A 250 mL Erlenmeyer flask was filled with 50 mL of seed culture solution, and five pieces of first-grade seed cakes of Sh-43 strain with a diameter of 5 mm were inserted, and cultured on a shaking table at 30 °C (220 r / min) for 120 h as secondary fermentation seeds. According to the scale of fermentation, for example, 2.5 L of fermentation medium in a 3.7 L fermentation tank, the inoculum size is 8 % (V / V), the rotation speed is 220 r / min, the temperat...

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Abstract

The invention, belonging to the technical field of agricultural microbiology, specifically relates to an anti-Rhizoctonia solani Streptomyces hygroscopicus Sh-43 strain, its fermentation solution production method and application. Streptomyces hygroscopicus Sh-43 strain is preserved in China Center for Type Culture Collection in Wuhan University under accession Number of CCTCC NO: M2012172 on May18, 2012. The Streptomyces hygroscopicus Sh-43 strain of the invention has the advantages of significant anti-Rhizoctonia solani effect, wide antimicrobial spectrum, stable resistance effect, simple culture conditions, and good control effect on rice sheath blight, and has wide application prospect in the field of biological control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbes, and in particular relates to a Streptomyces hygroscopicus Sh-43 strain antagonizing rice sheath blight and a production method and application of its fermented liquid. Background technique [0002] Rice sheath blight, commonly known as "flower foot disease", is one of the three major diseases of rice in my country. Especially in the high-yielding field of densely planted dwarf hybrid rice, the disease is most common and serious. It often leads to dead leaves, reduced seed setting rate, and reduced dry grain weight. Generally, diseased fields can cause a 15% to 20% reduction in yield, and in severe cases it can reach 60%. ~70 %, or even no harvest. The disease occurs in early, middle and late rice, and is extremely harmful. It generally occurs in various rice-growing areas in the world and has a tendency to increase year by year in recent years. [0003] Actinomycetes are named for...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/06A01N63/00A01N63/02A01P3/00C12R1/55
Inventor 蒋冬花崔宇辉王媛媛林葱葱金梦婷
Owner ZHEJIANG NORMAL UNIVERSITY
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