Arsenic-resistance related protein PvArrp1, and coding gene and application thereof

A resistance-related, genetic technology, applied in application, genetic engineering, plant genetic improvement, etc., to achieve the effect of enhancing resistance, great value, and reducing the accumulation of arsenic

Inactive Publication Date: 2012-10-24
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the widespread existence of arsenic in the environment, and there is no very effective method to remove arsenic in soil and groundwater in situ, it is of great significance to reduce the accumulation of arsenic in plants through biotechnology.

Method used

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  • Arsenic-resistance related protein PvArrp1, and coding gene and application thereof
  • Arsenic-resistance related protein PvArrp1, and coding gene and application thereof
  • Arsenic-resistance related protein PvArrp1, and coding gene and application thereof

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Experimental program
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Effect test

Embodiment 1

[0047] Embodiment 1, the invention of PvArrp1 protein and its coding gene

[0048] Centipede grass (Pteris vittata L.), belongs to the genus Pteris vittata of the fern family Pteridaceae.

[0049] The inventor discovered a protein related to arsenic resistance from Centipede grass, and named it PvArrp1 protein. The amino acid sequence of PvArrp1 protein is shown in sequence 1 of the sequence listing. The gene encoding PvArrp1 protein is named PvArrpl gene, and its open reading frame is shown in sequence 2 of the sequence listing.

Embodiment 2

[0050] Embodiment 2, the acquisition and identification of transgenic plants

[0051] 1. Construction of recombinant expression vector

[0052] 1. Extract the total RNA of the centipede grass blade.

[0053] 2. Reverse transcribe the total RNA extracted in step 1 into cDNA.

[0054] 3. Using the cDNA obtained in step 2 as a template, perform PCR amplification with a primer pair composed of PvArrp1S and PvArrp1A to obtain a PCR amplification product.

[0055] PvArrp1S: 5'-ATGGAGAACTCAAGCGCGGAGCGGA-3';

[0056] PvArrp1A: 5'-CTAAACAGAAGGCCCCTTCCTCTGA-3'.

[0057] PCR amplification program: 98°C for 10s, 55°C for 30s, 72°C for 70s, 35 cycles; 72°C for 10min; store at 10°C.

[0058] 4. Using the PCR amplification product in step 3 as a template, a primer pair composed of PvArrp1S1 / PvArrp1A1 is used to obtain a PCR amplification product.

[0059] PvArrp1S1: 5'- ggatcc ATGGAGAACTCAAGCGCGGAGCGGA-3';

[0060] PvArrp1A1: 5'- ggtacc CTAAACAGAAGGCCCCTTCCTCTGA-3'.

[0061] T...

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Abstract

The invention discloses arsenic-resistance related protein PvArrp1, and a coding gene and application thereof. The protein (PvArrp1 protein) provided in the invention is derived from Pteris vittata L. and is (a) protein composed of an amino acid sequence represented by sequence 1 in a sequence table, or (b) protein derived from sequence 1, wherein the protein is obtained through replacement and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence represented by sequence 1 in the sequence table and is related to resistance of plants to arsenic stress. When the coding gene of the protein provided by the invention is introduced into a plant, accumulation of arsenic in the plant can be substantially reduced, and resistance of the plant to arsenic stress is remarkably enhanced. According to the invention, effective approaches for breeding economic crops or crops with resistance to arsenic and low accumulation of arsenic are provided, and the invention is of a significant value to the field of agriculture.

Description

technical field [0001] The invention relates to an arsenic resistance-related protein PvArrp1, its coding gene and application. Background technique [0002] Arsenic is a toxic chemical element, and its common form is arsenate [Arsenate, As(V)] or arsenite [Arsenic, As(III)]. [0003] Arsenic pollution is a very prominent environmental problem at home and abroad, and my country is one of the countries with the most serious arsenic pollution in the world. Human economic activities can directly lead to relatively serious arsenic pollution, including ore smelting, coal burning, slag discarding, tanning waste, dye production, wood preservation, arsenic-containing pesticides, biochemical weapons and the use of insecticides. Arsenic in the environment is very harmful to humans, animals and plants, and can enter the human body through respiratory tract, food or skin contact. At present, arsenic pollution has caused epidemic diseases related to arsenic poisoning in many parts of th...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/21C12N15/82C12N15/84A01H5/00
Inventor 麻密陈焱山何振艳徐文忠
Owner INST OF BOTANY CHINESE ACAD OF SCI
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