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Molecularly imprinted sensor for fenvalerate, preparation method and detection method

A technology of fenvalerate and molecular imprinting, applied in the field of pesticide detection, can solve the problems of long preparation process, high preparation cost, complicated process and the like, and achieve the effects of low cost, high sensitivity and good selectivity

Inactive Publication Date: 2012-10-24
SHENZHEN POLYTECHNIC
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0007] Another technical problem to be solved by the present invention is to provide a method for preparing an electrochemical sensor for fenvalerate pesticide molecular imprinting, so as to overcome the problems of high production cost, complicated process and long time-consuming preparation process of detection equipment in the prior art
[0008] Another technical problem to be solved by the present invention is to provide a detection method for fenvalerate to overcome the problems of complicated operation, high cost and time-consuming detection in the existing detection technology

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  • Molecularly imprinted sensor for fenvalerate, preparation method and detection method
  • Molecularly imprinted sensor for fenvalerate, preparation method and detection method

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preparation example Construction

[0044] The preparation method of the fenvalerate molecularly imprinted electrochemical sensor of the present invention comprises the following steps:

[0045] (1) Pretreatment of gold electrodes;

[0046] (2) Preparation of the polymerization solution: prepare the acetonitrile solution of the functional monomer o-aminothiophenol, and add the acetonitrile solution of fenvalerate and the tetrabutylammonium perchlorate solution to prepare the polymerization solution;

[0047] (3) Preparation of polymer membrane: place the polymer solution in the electrolytic cell and suspend the gold electrode in the polymer solution, trigger the electrochemical polymerization reaction of o-aminothiophenol on the surface of the gold electrode, and gradually Generate a low-conductivity o-aminothiophenol polymer and deposit it on the surface of the gold electrode, and fenvalerate molecules are embedded in the polymer film, thereby preparing a modified electrode;

[0048] (4) Elute fenvalerate temp...

example 1

[0076] The preparation steps of the fenvalerate molecularly imprinted electrochemical sensor used in the present invention are as follows:

[0077] A) Pretreatment of gold electrodes:

[0078] The gold electrode (Ф=3㎜) was sequentially passed through 0.30μm and 0.05μm ɑ-Al 2 o 3 After polishing with the polishing powder suspension, ultrasonically clean it with absolute ethanol and deionized water for 2~5min respectively, and then scan the treated gold electrode in a 0.5 mol / L sulfuric acid solution at a potential of -0.3V~1.4V. Circle until a stable cyclic voltammetry response curve is obtained, take it out and rinse it with double distilled water for use;

[0079] B) Preparation of polymerization solution:

[0080] o-aminothiophenol, fenvalerate, and tetrabutylammonium perchlorate were dissolved in acetonitrile to obtain a polymer solution, wherein the concentration of o-aminothiophenol was 20mmol / L, and the concentration of fenvalerate was 5 mmol / L, the concentration of...

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Abstract

The invention relates to a detection method for fenvalerate, a molecularly imprinted sensor for fenvalerate and a preparation method for the sensor. The detection method comprises the following steps: providing a molecularly imprinted electrochemical sensor for fenvalerate and a DPV detection system thereof and fitting a concentration calculation formula; providing sample liquid to be detected; detecting the peak current I[sample] of the sample liquid; and calculating the concentration of fenvalerate. The molecularly imprinted sensor comprises a gold electrode used as a substrate and a molecularly imprinted sensor membrane for fenvalerate prepared on the surface of the gold electrode by using an electropolymerization method, wherein the sensor membrane retains molecularly imprinted holes used for recognition of fenvalerate molecules. The preparation method for the sensor comprises the following steps: pretreating the gold electrode; preparing polymer fluid; preparing the polymer membrane; and eluting fenvalerate template molecules. According to the invention, an electrochemical detection way is employed in the invention; a great amount of complex pretreatment work is avoided, used apparatuses are simple, cost is low, and detection of fenvalerate in an on-site environment is realized. The sensor provided in the invention has the advantages of a simple preparation process, good selectivity and high sensitivity.

Description

technical field [0001] The invention relates to the technical field of pesticide detection, in particular to a detection method of fenvalerate, a molecular imprinted sensor and a preparation method of the sensor. technical background [0002] Pyrethroid pesticides are widely used, and fenvalerate is one of the main ingredients. At present, the detection methods of fenvalerate pesticide residues are mainly chromatography and immunoassay. [0003] The chromatographic method includes the following steps: 1. Preparation of pesticide standard samples; 2. Sample preparation; 3. Sample extraction and purification; 4. Chromatographic analysis. The extraction process of Step 3 is as follows: Weigh 25.0 g of the mashed sample, add 100 mL of the solution formed by petroleum ether: acetone = 1:1, place it in a tissue masher for homogenate extraction for 2 minutes, vacuum filter, and mix with 25 mL respectively The filter residue was washed with solvent, and the filtered solution was e...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 栾崇林
Owner SHENZHEN POLYTECHNIC
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