Chinese medicinal herb mixture for preventing and treating new castle disease and production technology
A technology of chicken Newcastle disease and Chinese herbal medicine, which is applied in the direction of medical formulas, antiviral agents, and medical preparations containing active ingredients, etc., to achieve the effect of no toxic side effects, good control effect, and strong inhibitory effect
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Embodiment 1
[0028] Proportions (parts by weight): Honeysuckle 8, Daqingye 8, Banlangen 10, Scutellaria baicalensis 8, Forsythia 10, Gypsum 19, Anemarrhena 10, Gardenia 8, Campanulaceae 7, Bitter almond (fried) 5, Fritillaria 3 , Licorice 4.
[0029] The production process is:
[0030] (1) Soaking: Weigh each raw material by weight for later use; soak honeysuckle, Folium Folium, and Scutellaria baicalensis in 6 times the amount of warm water (40-50°C) for 1 hour; break raw gypsum into single packets; Anemarrhena, Gardenia, Campanulaceae, Bitter Almond, Fritillaria, Licorice, add 6 times the amount of warm water (40-50°C) and soak for 1 hour.
[0031] (2) Decoction: Decoct raw gypsum for 30 minutes, then add 8 traditional Chinese medicines such as Radix Isatidis and Forsythia (together with soaking water) and boil for 30 minutes, then add honeysuckle, Daqingye and Scutellaria baicalensis (together with soaking water) and boil again After 30 minutes, filter; add water to the filter residue...
Embodiment approach 1
[0036] Embodiment 1 Anti-Newcastle Disease Virus Experiment of Chicken Embryos
[0037] 1. Test materials and methods
[0038] 1.1 11-day-old SPF chicken embryos were provided by Shandong Haotai Experimental Animal Center. NDV-LaSota (EID 50 for 10 -6.0 / 0.1mL), provided by the Key Laboratory of Poultry Diseases of Shandong Province. Amantadine hydrochloride, batch number 200602851, produced by Northeast Pharmaceutical General Factory. PBS was made into a 0.1% solution for later use. Viralin, produced by Liaoning General Pharmaceutical Factory, batch number 200602049, prepared as a 0.1% solution in PBS for later use.
[0039] 1.2 The chicken embryos were randomly divided into Chinese medicine group, western medicine control group, normal saline control group and virus control group, with 15 embryos in each group and 4 consecutive repetitions. Firstly mix the traditional Chinese medicine with an equivalent amount of 100EID 50 Mix the virus liquid at room temperature for ...
Embodiment approach 2
[0051] Embodiment 2 Effect of traditional Chinese medicine on movement of chicken tracheal cilia
[0052] 1. Materials SPF rooster: 40 days old, average weight 290g; medicines and reagents: urethane, Chinese ink, cold light flashlight, vernier calipers, hemostat, scissors, sterilized absorbent cotton, etc.
[0053] 2. Methods 20 cocks were randomly divided into Chinese medicine group of the present invention and normal saline group, 10 in every group. The traditional Chinese medicine group was fed with 0.3 mL / time, 2 times / day, for 3 consecutive days, and the normal saline group was fed with the same amount of normal saline. On the 4th day, the feed was stopped for 8 hours, and anesthetized by intraperitoneal injection of 20% ethyl carbamate solution 3000 mg / kg body weight. After deep anesthesia, it was lowered and fixed on a special platform, the neck was fixed and stretched, the skin and hair of the neck were disinfected, the hair of the neck was cut off, the skin of the ne...
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