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Low-molecular weight schisandra chinensis polysaccharides and preparation method and application thereof

A low-molecular-weight, schisandra technology, applied in the field of medicine, can solve the problems of no patent disclosure and no domestic and foreign literature reports, and achieve the effects of low cost, improving the immune function of the body, improving autoimmunity and antioxidant activity in the body

Inactive Publication Date: 2012-10-31
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no domestic and foreign literature reports on the in vivo anti-human liver cancer cell HepG-2 of the purified fraction of Schisandra chinensis polysaccharides, and no corresponding patent publications

Method used

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  • Low-molecular weight schisandra chinensis polysaccharides and preparation method and application thereof
  • Low-molecular weight schisandra chinensis polysaccharides and preparation method and application thereof
  • Low-molecular weight schisandra chinensis polysaccharides and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The raw materials were washed with water, dried at 60°C, pulverized, placed in a Soxhlet extractor, degreased with petroleum ether (distillation range 60-90°C) for 15 hours, filtered, and the filter residue was dried at 60°C for later use. Weigh 100g defatted Schisandra chinensis powder, put it in a round bottom flask, reflux and extract once in a hot water bath according to the ratio of material to liquid 1:8, extraction time 3h, extraction temperature 80°C, cool and centrifuge, combine the above Clear liquid: the above clear liquid is concentrated under reduced pressure, and 4 times the volume of 95% ethanol is added for precipitation, so that the concentration of ethanol in the concentrated solution of the polysaccharide is 80%, and the obtained precipitate is centrifuged and freeze-dried for 24 hours to obtain the crude polysaccharide sample powder of Schisandra chinensis; An appropriate amount of crude polysaccharide from Schisandra chinensis is reconstituted, and t...

Embodiment 2

[0024]The raw materials were washed with water, dried at 60°C, pulverized, placed in a Soxhlet extractor, degreased with petroleum ether (distillation range 60-90°C) for 15 hours, filtered, and the filter residue was dried at 60°C for later use. Weigh 100g of defatted schisandra powder, put it in a round bottom flask, reflux in a hot water bath for 2 times according to the material-to-liquid ratio of 1:10, extraction time of 4 hours, and extraction temperature of 90°C, then centrifuge after cooling, and combine Clear liquid: the above clear liquid is concentrated under reduced pressure, and 4 times the volume of 95% ethanol is added for precipitation, so that the concentration of ethanol in the concentrated solution of the polysaccharide is 80%, and the obtained precipitate is centrifuged and freeze-dried for 36 hours to obtain the crude polysaccharide sample powder of Schisandra chinensis; An appropriate amount of crude polysaccharide from Schisandra chinensis is reconstituted...

Embodiment 3

[0026] The raw materials were washed with water, dried at 60°C, pulverized, placed in a Soxhlet extractor, degreased with petroleum ether (distillation range 60-90°C) for 15 hours, filtered, and the filter residue was dried at 60°C for later use. Weigh 100g defatted Schisandra chinensis powder, put it in a round bottom flask, reflux and extract it in a hot water bath for 3 times according to the ratio of solid to liquid 1:12, extraction time 5h, extraction temperature 100°C, after cooling, centrifuge, combine the Clear liquid: the above clear liquid is concentrated under reduced pressure, and 4 times the volume of 95% ethanol is added for precipitation, so that the concentration of ethanol in the concentrated solution of the polysaccharide is 80%, and the obtained precipitate is centrifuged and freeze-dried for 48 hours to obtain the crude polysaccharide sample powder of Schisandra chinensis; An appropriate amount of crude polysaccharide from Schisandra chinensis is reconstitut...

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Abstract

The invention discloses low-molecular weight schisandra chinensis polysaccharides and a preparation method and an application thereof, and belongs to the technical field of medicine. The molecular weight of the polysaccharide component is 3.4*10<3> Da, and the monosaccharide composition is as follows: mannose:glucose:galactose=1:11.38:3.55; and the polysaccharide component contains (1-3)-alpha-glucan and has a tri-helix structure. The study of in vivo activity against liver cancer of SCPP11 polysaccharide component shows that the polysaccharide component is significantly active against liver cancer (HepG-2), can significantly inhibit growth of liver cancer, and can significantly enhance the autoimmunity and in vivo antioxidant activity of mice as compared with the 5-Fu. The water-soluble low-molecular weight schisandra chinensis polysaccharides have the advantages of definite active components and controllable quality, and can be used as a drug against liver cancer or a health-care product for improving immunity.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a preparation method of low-molecular-weight schisandra polysaccharide and its application in anti-liver cancer and improving body immunity. Background technique [0002] Malignant tumors are recognized as one of the most serious diseases to human health in the world, second only to cardiovascular diseases. According to the GLOBOCAN 2008 statistical report of the International Agency for Research on Cancer (Jemal, A., Bray, F., Center, M.M., Ferlay, J., Ward, E., Forman, D.. Global Cancer Statistics. CA: A Cancer Journal for Clinicians. 2011, 61(2), 69-90; GLOBOCAN 2008, Cancer Incidence and Mortality Worldwide: International Agency for Research on Cancer; Available at: http: / / globocan.iarc.fr / .), in 2008, there were 12.7 million new cancer cases worldwide, and 7.6 million people died of cancer. It is predicted that by 2030, cancer deaths are expected to increase by 72%, and the...

Claims

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Application Information

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IPC IPC(8): C08B37/00A23L1/09A61K31/715A61P35/00
Inventor 仰榴青赵婷吴向阳邹烨茆广华李芳任月娜
Owner JIANGSU UNIV
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