Method for eliminating dependence of methanol-induced promoter on single methanol carbon source

Abstract

The invention relates to a method for eliminating dependence of a methanol-induced promoter on a single methanol carbon source. By means of increasing the expression index of an Mit1 polypeptide in a methylotrophic yeast cell and activating the expression of a promoter to be induced by methanol, the promoter which is originally dependent on the methanol for induction can be also used for expressing foreign polypeptides without depending on single methanol.

Description

technical field

[0001] The invention belongs to the field of bioengineering; more specifically, the invention relates to a method for expressing exogenous polypeptides by eliminating the single methanol carbon source dependence of methanol-inducible promoters. Background technique

[0002] The expression system of methanolotrophic yeast (including Pichia, Hansenula, Candida, Torulopsis, etc.) has been widely used in industrial production, pharmaceuticals and other fields due to its high-efficiency expression ability of exogenous polypeptides. Its characteristic is that they have promoters that can be efficiently induced by methanol (AOX1 promoter, DHAS promoter, FDH promoter, MOX promoter, AOX2 promoter, ZZA1, PEX5-, PEX8-, PEX14-promoter, etc.), And these promoters are strictly dependent on methanol, other carbon sources (such as glucose, glycerol, etc.) will inhibit the expression of these promoters.

[0003] With the outbreak of the oil crisis, the cost of using methanol...

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