Tissue culture method for Polygonaturm sibiricum Redoute

A technology of tissue culture and sealwort, which is applied in the field of plant tissue culture, can solve the problems of low supply, long natural cultivation period, and low yield of wild sealwort, so as to improve efficiency, increase the efficiency and quantity of reproduction, and increase the sterility rate and the effect on survival rate

Active Publication Date: 2012-11-14
金寨森沣农业科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a tissue culture method of Rhizoma Polygonatum, through which Polygonatum Rhizoma can be rapidly propagated, a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Selection and sterilization of explants

[0029]Select the young shoots on the rhizomes of Polygonatum spp., dig out the young shoots from the soil and cut the young shoots, quickly wash away the sediment with tap water, rinse with a toothbrush, soak in detergent (commercially available conventional washing powder) for 30 minutes and use Gently brush off the dirt on the surface with a soft brush, remove the outer layer of bracts, and leave the innermost layer of milky white buds, rinse with tap water for 3 hours, and place on an ultra-clean workbench: sterilize with a volume concentration of 75% alcohol solution for 15 seconds, and then aseptic Rinse with water 3 times, volume concentration 0.2%Hgcl 2 , Hgcl 2 The solution contains Tween 20 with a volume concentration of 0.15%, soaked for 12 minutes, rinsed with sterile water 5 times, transferred to a petri dish to blot the water and waited to be inserted into the culture medium; the Hgcl mixed with Tween 20 2 The...

Embodiment 2

[0037] (2) Selection and sterilization of explants

[0038] Select the tuber part of the current year (that is, grown in the first year after planting) with hidden bud points, and cut the tuber into 1.0cm 3 Left and right small pieces, and each piece must have at least one hidden bud point, quickly wash away the sediment with tap water, use a toothbrush to assist in the rinse, soak in detergent solution for 30 minutes and gently brush off the surface dirt with a soft brush, rinse with tap water for 3 hours, Put it on the ultra-clean workbench: Sterilize with 75% alcohol solution for 15 seconds, then wash it with sterile water for 3 times, with a volume concentration of 0.2% Hgcl 2 , Hgcl 2 The solution contains Tween 20 with a volume concentration of 0.2%, soaked for 14 minutes, rinsed with sterile water 5 times; transferred to a petri dish to blot up the water and waited to be inserted into the culture medium. Hgcl mixed with Tween 20 2 The disinfection effect of the solut...

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PUM

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Abstract

The invention discloses a tissue culture method for Polygonaturm sibiricum Redoute. The tissue culture method comprises the following steps of: performing cluster bud induction, rooting culture and seedling exercising on buds of a subterranean stem which is grown in the current year, and then transplanting the buds; and performing callus culture, multiplication culture, rooting culture and seedling exercising on a tuber part which is grown in the current year and has hidden bud points, and then transplanting the tuber part. The method can be used for quickly reproducing the Polygonaturm sibiricum Redoute, realizes the large-area industrialized planting of the Polygonaturm sibiricum Redoute, and can be used for solving the problems of low yield, less supply and long natural cultivation period of wild hid Polygonaturm sibiricum Redoute.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for tissue culture of Rhizoma Polygonatum. Background technique [0002] Polygonaturm sibiricum Redoute, also known as Chicken Head Polygonatum and Chicken Head Ginseng, belongs to the perennial herb of the genus Polygonatum in the family Liliaceae. Traditional Chinese medicine uses Polygonatum rhizome as medicine, which has the effects of nourishing the spleen and lungs, nourishing qi and nourishing yin, and strengthening bones and muscles. The main ingredients are polygonatum polysaccharide and saponin. [0003] Dizang Polygonatum is a unique wild Polygonatum species in Jiuhuashan, Anhui. It has extremely high medicinal and economic value. As the medicinal value and health value of Polygonatum are more and more recognized by people, the demand for raw materials of Polygonatum is also increasing. The unplanned collection of wild sealwort intensified, resulting in a...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
Inventor 项艳任洁赵华琳沈周高汤锋王进
Owner 金寨森沣农业科技开发有限公司
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