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Novel insecticidal and antibacterial biofertilizer and preparation method thereof

A biological fertilizer, a new type of technology, applied in the field of biological fertilizers, can solve the problems of fertilizer efficiency and side effects, and achieve the effect of promoting material transformation, high efficiency, and eliminating harmful substances

Active Publication Date: 2012-11-14
山西神宇惠农科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microbial fertilizer itself relies on microbial activities to fix nitrogen, decompose phosphorus, and decompose potassium, and the addition of microorganisms that can produce toxins will inevitably have certain side effects on fertilizer efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A novel insecticidal and antibacterial biological fertilizer and a preparation method thereof, the steps are:

[0025] (1) Strain activation: The preserved strains of the three strains were inoculated on their respective seed slant medium, Bacillus megaterium and Rhodopseudomonas palustris were cultured at 30°C for 48 hours, and Streptomyces avermitilis were cultured at 28°C for 14 days.

[0026] (2) Seed culture: Separately pack the seed culture medium of 3 strains in 1L Erlenmeyer flasks, sterilize at 120°C for 30 minutes, inoculate the respective slant seeds after cooling to room temperature, shake and cultivate in a constant temperature shaker, Streptomyces avermitilis at 30°C , cultured at 180r / min for 48 hours, microscopic examination showed no bacteria, mycelia were strong, and the pH of the seed solution was about 6.5; Bacillus megaterium and Rhodopseudomonas palustris were cultured at 200r / min for 14-18 hours at 30°C, and the microscopic examination showed that ...

Embodiment 2

[0061] Preparation method is with embodiment 1 each raw material ratio is

[0062] 550 million / ml Bacillus megaterium 30 parts by weight

[0063] 30 parts by weight of 400 million / ml Rhodopseudomonas palustris

[0064] Streptomyces avermitilis active ingredient B at 562mg / mL 1a 7 parts by weight

[0065] Molasses 300 parts by weight

[0066] Sodium dodecylbenzenesulfonate 0.03 parts by weight

[0067] Tween-80 0.05 parts by weight

[0068] 600 parts by weight of water

[0069] Fertilizer efficiency and bactericidal and insecticidal effect test

[0070] 1 Test site: eggplant garden in the suburbs of Taiyuan, Shanxi

[0071] 2 Test time: May 2011

[0072] 3 Test object: Eggplant

[0073] 4 Formula: Spray 300 times of the stock solution of the new insecticidal and antibacterial biological fertilizer 4 times on May 11, 21, June 5, and 20 respectively

[0074] 5 test plan:

[0075] In the suburbs of Taiyuan, a field with fertile soil and uniform soi...

Embodiment 3

[0081] Preparation method is the same as embodiment 1

[0082] 500 parts by weight of 550 million / ml Bacillus megaterium

[0083] 10 parts by weight of 400 million / ml Rhodopseudomonas palustris

[0084] Streptomyces avermitilis active ingredient B at 562mg / mL 1a 13 parts by weight

[0085] Molasses 300 parts by weight

[0086] Sodium dodecylbenzenesulfonate 0.04 parts by weight

[0087] Tween-80 0.03 parts by weight

[0088] Water 800 parts by weight

[0089] Fertilizer efficiency and bactericidal and insecticidal effect test

[0090] 1 Test location: Lishuyuan in the suburbs of Taiyuan, Shanxi

[0091] 2 Test time: April 2011

[0092] 3 Test object: pear tree

[0093] Formula: The stock solution of the new insecticidal and antibacterial biological fertilizer is sprayed at a concentration of 500 times, and sprayed at the full flowering stage, sprayed once every 7 days, and sprayed 3 times in total.

[0094] 5 test plan:

[0095] In the outskirts...

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Abstract

The invention relates to a biofertilizer, particularly a novel insecticidal and antibacterial biofertilizer and a preparation method thereof. The invention enhances the bactericidal and insecticidal effects and fertilizer efficiency of the microbial fertilizer. The novel insecticidal and antibacterial biofertilizer is prepared from 30-50 parts of 550 million / ml Bacillus megaterium, 10-30 parts of 400 million / ml Rhodopseudomonas palustris, 1-3 parts of 562mg / mL Streptomyces avermitilis drug effect component B1a, 200-300 parts of molasses, 0.02-0.04 part of sodium dodecyl benzene sulfonate, 0.03-0.05 part of Tween-80 and 600-800 parts of water. The insecticide / fertilizer compound biological agent and the production technique thereof establish technical parameters of fermentation of Bacillus megaterium, Rhodopseudomonas palustris and Streptomyces avermitilis, and all the indexes satisfy the quality standard of microbial fertilizers of Department of Agriculture. The insecticide / fertilizer compound biological agent provided by the invention has insecticidal and antibacterial effects, and has wide application prospects.

Description

technical field [0001] The invention relates to a biological fertilizer, in particular to a novel insecticidal and antibacterial biological fertilizer and a preparation method thereof. Background technique [0002] Microbial fertilizers are fertilizers prepared from one or several living cells of beneficial microorganisms. There are mainly rhizobia agents, nitrogen-fixing bacteria agents, phosphorus bacteria agents, antibiotic agents, compound bacteria agents, etc. The production cost of microbial fertilizer is low, the application effect is good, and it does not pollute the environment. After use, it not only increases production, but also improves the quality of agricultural products. Therefore, it plays an important role in the sustainable development of agriculture in my country. With the development of ecological agriculture and green agriculture, people pay more and more attention to the harm of chemical pesticides and chemical fertilizers to soil and human beings. Re...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/02C05G3/60
Inventor 高秀红
Owner 山西神宇惠农科技有限公司
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