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Complex strain, fermentation agent and preparation method of natural fermented kim milk

The technology of skim milk and fermented milk is applied to the preparation of active yoghurt, and its application, the preparation of pure natural fermented skim milk, and the preparation of starter, can solve the problem that the action time is difficult to control, the embedding process is complicated, and the content of lactose is reduced. and other problems, to achieve the effect of enriching biological activity and live probiotics, optimistic market prospects, and easy to obtain

Active Publication Date: 2012-11-14
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are four problems: 1) Most of the lactases provided on the market are enzymes with strong hydrolysis ability and weak transglycoside ability, which are not suitable for the production of GOS; 2) The lactase produced by the laboratory has a very Complicated, including the extraction and purification of β-galactosidase, this process introduces some other separation equipment, media, salt or organic solvents, and its safety cannot be guaranteed; Therefore, it is difficult to grasp the stability of the product; 4), in the patent "a production method of milk rich in galacto-oligosaccharides" [13], it involves a carrier particle with a hydrazide group to β- Embedded with galactosidase, the prepared product not only contains GOS, but also reduces the content of lactose
The embedding process is complicated and not suitable for industrial production
The specific chemical names of the particles with hydrazide groups are not described in this article, and their chemical safety needs to be further verified
The second technology is to directly add the commercially available functional food additive GOS, which undoubtedly increases the cost of the product, and has a process defect, that is, after adding GOS, the shelf life stability of the product is not good, and high-speed shearing, Stirring [14], which further increases the cost from power consumption
There are no fermented dairy products prepared based on GOS on the domestic and international markets

Method used

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  • Complex strain, fermentation agent and preparation method of natural fermented kim milk
  • Complex strain, fermentation agent and preparation method of natural fermented kim milk
  • Complex strain, fermentation agent and preparation method of natural fermented kim milk

Examples

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preparation example Construction

[0085] A preparation method of pure natural fermented skim milk containing GOS, comprising the following steps:

[0086] a) dissolving skim milk powder in ultrapure water to prepare a homogeneous solution to obtain reconstituted skim milk; the percentage of skim milk powder to the mass volume (g / mL) of ultrapure water is 10-14%, preferably 12%;

[0087] b) Sterilize the reconstituted skim milk, and then cool it to obtain sterilized reconstituted skim milk; the sterilization temperature is 95-10°C, preferably 95°C, and the time is 14.5-15.5min, preferably 15min;

[0088] c) inoculate the starter described in this specific embodiment into sterilized reconstituted skim milk, and stir evenly; the mass percentage of the starter in the reconstituted skim milk is 9.8-10.2%, preferably 10%;

[0089] d) Fermentation to obtain pure natural fermented skim milk containing GOS; the fermentation method is anaerobic fermentation or ordinary fermentation, and the temperature of the anaerobic ...

Embodiment

[0091] 1. Equipment

[0092] Digital display overhead stirrer (IKA, RW20Digital), ultra-clean table (Shanghai Boxun Industrial Co., Ltd., BJ-2CD), magnetic stirring heater (IKA, C-MAG HS7), milk cup (Avent VIA, commercially available), Digital rotational viscometer (Changsha Dalong Instrument Equipment Trade Co., Ltd., SNB-1 type), pH meter, ion chromatography with amperometric detector connected in series, gradient pump and autosampler (Dionex, ICS3000), chromatographic separation column (Dionex , CarboPac PA20) and guard column, high-speed centrifuge (Shanghai Lu Xiangyi Centrifuge Instrument Co., Ltd., GL-22M).

[0093] Second raw material

[0094] Skim milk powder (NZMP Skimmilk Powder, supplied by Fonterra Trading (Shanghai) Co., Ltd.), Lactobacillus bulgaricus (Danisco, LB340), Lactobacillus acidophilus (Danisco, TA40 and TA45), lactase F (Amano Enzyme), acetonitrile ( Shanghai AMP Company); other conventional reagents were of analytical grade and purchased from Sinoph...

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Abstract

The invention relates to a complex strain and also relates to application thereof. The invention also relates to a fermentation agent and a preparation method thereof. The invention also relates to a preparation method of active yoghurt, in particular to a preparation method of galactooligosaccharide-containing natural skim fermented milk. The complex strain comprises lactobacillus bulgaricus LB340 and streptococcus thermophilus. The complex strain is used for preparing the galactooligosaccharide-containing natural skim fermented milk. The strain of the fermentation agent is the complex strain. The preparation method of the galactooligosaccharide-containing natural skim fermented milk uses to fermentation agent for fermentation. The invention solves the preparation problem of the galactooligosaccharide-containing natural skim fermented milk.

Description

technical field [0001] On the one hand, the present invention relates to a compound strain and its application; on the other hand, it relates to a starter, and also to a preparation method of the starter; on the other hand, the present invention relates to a preparation of active yogurt The method is specifically a method for preparing pure natural fermented skim milk containing galacto-oligosaccharides (GOS). Background technique [0002] Galacto-oligosaccharides (Galacto-oligosaccharides, GOS) refer to the galactose side of galactose or lactose, linked by β(1→3), β(1→4), β(1→6) bonds, and A binding sugar bound with 1 to 7 or 1 to 6 molecules of galactose, with β (1→4) bonds as the main intermolecular galactose. In nature, the content of GOS in breast milk is slightly more, about 5-8gL -1 , GOS content in ruminant milk is very small: GOS content in goat milk is 250-300mgL -1 , respectively 4 to 5 times that of cow milk and sheep milk [1-2]. [0003] GOS is a biologicall...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/127C12R1/225C12R1/46
Inventor 高艳玲乐卫东米晓冲李德龙张晓洁郭琪祯张波王志广
Owner SHANGHAI JIAO TONG UNIV