Coding suspension microchip and preparation method and application thereof

A microchip, coding technology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve the problems of difficult operation, environmental pollution, high cost, etc.

Active Publication Date: 2012-11-21
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to implement this technology. For example, it is necessary to deposit titanium, gold and other precious metals to form a metal film by means of expensive electron beam evaporation, and it is necessary to use highly corrosive hydrofluoric acid to dissolve the metal film for engraving, which is expensive. , the operation is not easy, and it will bring many problems such as environmental pollution, and it is necessary to cover the metal film with transparent polymer materials to connect biological probe molecules, etc., the process is complicated

Method used

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  • Coding suspension microchip and preparation method and application thereof
  • Coding suspension microchip and preparation method and application thereof
  • Coding suspension microchip and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0063] refer to figure 1 , the preparation method of the coded suspension microchip may include the following steps:

[0064] (1) forming an Al sacrificial layer 4 on the substrate 3;

[0065] (2) SiO is formed on the surface of the sacrificial layer 2 layer 10;

[0066] (3) forming a material layer 20 that is sufficiently opaque to visible light on the surface of the transparent material layer;

[0067] (4) Perform photoresist coating, light development, etching, and cleaning on the material layer that is sufficiently opaque to visible light in sequence, and form an opaque planar microstructure used as a graphic identification code on the material layer that is sufficiently opaque to visible light;

[0068] (5) Select a solution that can specifically dissolve the sacrificial layer (for example, dilute hydrochloric acid, but not limited thereto), and immerse the device prepared in the preceding step (4) in the solution to peel off the substrate to obtain the target product....

Embodiment 1

[0078] Example 1 Detection of multiple nucleic acid amplification products in biological samples (nucleic acid detection)

[0079] Combined with conventional polymerase amplification reaction (PCR) technology, the amplification of multiple template molecules in the same sample can be easily realized by using the coded suspension microchip. Since there is no need to use multiple fluorescent channels to distinguish different amplification products, this application avoids the limitation of the number of fluorescent channels in existing multiplex PCR instruments (generally only 4), and can significantly increase the throughput of PCR; at the same time, it does not require Specially design and synthesize multiple probes linked to different fluorophores, and there is no need to use expensive multi-channel real-time quantitative PCR instruments, which can greatly reduce the cost of use.

[0080] Implementation steps:

[0081] First, the number of different templates to be detecte...

Embodiment 2

[0088] Example 2 Label-free multiplex detection (nucleic acid detection) using molecular beacon probes

[0089] For label-free detection without amplification reactions, molecular beacons known in the art can be used as probes. It is characterized in that the detection signal can be directly triggered by the binding event of the target and the probe without adding additional indicator.

[0090] Implementation steps:

[0091] The probe is determined according to the specific conditions of the nucleic acid to be detected in the sample. Samples that may be used with the method include, but are not limited to, solutions, cell extracts, whole blood, serum, urine, saliva, forensic specimens, and the like. A specific molecular beacon is designed for the sequence of each nucleic acid to be detected.

[0092] Firstly, the coded suspension microchip required for detection is prepared, and corresponding probes are modified on its surface. Its specific process is as described in Exa...

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Abstract

The invention discloses a coding suspension microchip and a preparation method and application thereof. The microchip comprises a transparent substrate and a non-transparent plane microstructure serving as graph identification codes, and the non-transparent plane microstructure is distributed on the surface of the transparent substrate. Preferentially, the non-transparent plane microstructure comprises a high light reflection stacking structural layer formed on the surface of the transparent substrate. The microchip can be manufactured through a semiconductor micromachining process, an application mode of the microchip is that probe molecules suspended on the microchip in a liquid phase system are used for capturing molecules of objects to be measured, then the microchip is separated from the liquid phase system and washed, and finally images of the microchip are formed in a visible light channel and a fluorescence channel respectively to determine identification of target molecules captured by the microchip and conduct quantitative analysis on the target molecules. Inexpensive manufacture of the coding suspension microchip with high throughput and good detection accuracy is achieved, and the coding suspension microchip can be applied to simultaneous detection of multiple biology/medical indexes in liquid samples.

Description

technical field [0001] The invention relates to a microsuspension chip and a preparation process thereof, in particular to a coded suspension microchip, its preparation method and application. Background technique [0002] Suspension chip technology (SAT) is a new type of high-throughput biochip technology. As a detection carrier, the ball has the characteristics of high throughput, fast speed, high sensitivity, strong specificity and wide detection range. In recent years, suspension chip technology has been widely used in immunology, genomics, proteomics and clinical diagnostic testing. However, referring to US Patent US2007037195 A1, the existing suspension chips still have many defects, and it is difficult to fully meet the needs of practical applications. [0003] For example, U.S. Patent No. 8,148,139B2 discloses a light transmission detection microsphere (Light Transmitted Assay Beads, LITAB), which uses a partially hollowed-out metal film as the coding layer, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/75G01N21/76
Inventor 李炯郑克孝姜丽沈叶
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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