Coding suspension microchip and preparation method and application thereof

A microchip, coding technology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve the problems of difficult operation, environmental pollution, high cost, etc.
CN102788779AActive Publication Date: 2012-11-21SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
Publication Date
2012-11-21

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Abstract

The invention discloses a coding suspension microchip and a preparation method and application thereof. The microchip comprises a transparent substrate and a non-transparent plane microstructure serving as graph identification codes, and the non-transparent plane microstructure is distributed on the surface of the transparent substrate. Preferentially, the non-transparent plane microstructure comprises a high light reflection stacking structural layer formed on the surface of the transparent substrate. The microchip can be manufactured through a semiconductor micromachining process, an application mode of the microchip is that probe molecules suspended on the microchip in a liquid phase system are used for capturing molecules of objects to be measured, then the microchip is separated from the liquid phase system and washed, and finally images of the microchip are formed in a visible light channel and a fluorescence channel respectively to determine identification of target molecules captured by the microchip and conduct quantitative analysis on the target molecules. Inexpensive manufacture of the coding suspension microchip with high throughput and good detection accuracy is achieved, and the coding suspension microchip can be applied to simultaneous detection of multiple biology / medical indexes in liquid samples.
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Description

technical field

[0001] The invention relates to a microsuspension chip and a preparation process thereof, in particular to a coded suspension microchip, its preparation method and application. Background technique

[0002] Suspension chip technology (SAT) is a new type of high-throughput biochip technology. As a detection carrier, the ball has the characteristics of high throughput, fast speed, high sensitivity, strong specificity and wide detection range. In recent years, suspension chip technology has been widely used in immunology, genomics, proteomics and clinical diagnostic testing. However, referring to US Patent US2007037195 A1, the existing suspension chips still have many defects, and it is difficult to fully meet the needs of practical applications.

[0003] For example, U.S. Patent No. 8,148,139B2 discloses a light transmission detection microsphere (Light Transmitted Assay Beads, LITAB), which uses a partially hollowed-out metal film as the coding layer, and th...

Claims

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