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Method for preparing anti-oxidization active camellia olefera cake polysaccharide

A technology of antioxidant activity and camellia oleifera cake, which is applied in the field of biochemistry, can solve the problems of high energy consumption, long-term heat decomposition of polysaccharides, etc., and achieve the effects of energy saving, process safety and environmental protection, and high antioxidant activity

Inactive Publication Date: 2012-11-28
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because it needs to be heated to boiling, it consumes a lot of energy, and at the same time, it will cause the polysaccharide to decompose if it is heated for too long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of Camellia oleifera cake polysaccharide:

[0024] Weigh 250g of Camellia oleifera cake and pulverize it to 80 mesh powder, add 5000mL of water, add 875mg of cellulase (enzyme activity > 15000u / g), extract in a water bath at 50°C for 3 hours, and filter with double gauze to obtain a light yellow extraction solution. The resulting extract was concentrated to 700ml under reduced pressure, so that the mass percentage of the solute in the solution was 6%, passed through a chromatographic column prepacked with 500g polyamide, fully mixed, left to stand for adsorption for 12 hours, and removed with 4 times the column volume deionized water for elution. The obtained elution solution was collected, concentrated under reduced pressure to a smaller volume, 95% ethanol five times the volume of the concentrated solution was added, fully stirred, and allowed to stand for 12 hours. Centrifuge (4500 rpm, 10 min). The precipitate was taken and vacuum freeze-dried to o...

Embodiment 2

[0030] (1) Preparation of Camellia oleifera cake polysaccharide:

[0031] Weigh 250g of camellia oleifera cake and pulverize it to 80 mesh powder, add 7500mL of water, add 1000mg of cellulase (enzyme activity > 15000u / g), extract in a water bath at 55°C for 5 hours, filter with double-layer gauze, and obtain a light yellow extraction solution. The obtained extract was concentrated to 1000ml under reduced pressure, so that the mass percentage of the solute in the solution was 4%, passed through a chromatographic column prepacked with 500g polyamide, fully mixed uniformly, left to stand for adsorption for 10 hours, and removed with 4 times the column volume deionized water for elution. The obtained eluted solution was collected, concentrated under reduced pressure to a smaller volume, 95% ethanol four times the volume of the concentrated solution was added, fully stirred, and allowed to stand for 24 hours. Centrifuge (4500 rpm, 10 min). The precipitate was taken and vacuum fre...

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Abstract

The invention relates to a method for extracting polysaccharide, in particular to an anti-oxidization polysaccharide extraction method in which camellia olefera cakes are used as raw materials. The method comprises the following specific steps: crushing the camellia olefera cakes to 60-80 meshes; adding into an aqueous solution with pre-dissolved cellulase (the enzymatic activity is greater than 15,000 u / g) for extraction, wherein the mass ratio of the cake powder to the aqueous solution is 1: (20-40); the using amount of the cellulase is 0.3-0.4% of the weight of the cake powder; the extraction temperature is 45-55 DEG C; and the extraction time is 3-5 hours; filtering the extract, concentrating, adsorbing by using polyamide for 8-12 hours, and eluting with deionized water; collecting the eluent, concentrating, adding 3-5 times of ethanol, and precipitating for 12-36 hours; and centrifugating to obtain precipitates after ethanol precipitation, and freezing and drying to obtain the grey white polysaccharide. According to the method, camellia olefera processing waste is fully utilized; the technical process is safe and environment-friendly; no toxic chemical reagents are used; and a new way for fully utilizing the camellia olefera processing waste is supplied.

Description

technical field [0001] The invention relates to the field of biochemistry, and relates to a method for extracting polysaccharides, in particular to a method for extracting antioxidant polysaccharides using camellia oleifera cake as raw material. Background technique [0002] Plant polysaccharides have many biologically active functions, including immune regulation, anti-tumor, hypoglycemic, hypolipidemic, anti-radiation, antibacterial and antiviral, liver protection, etc. The extensive biological activities of plant polysaccharides have been gradually utilized by people, and their unique activities and natural sources have great potential in the application of human health protection. Therefore, how to improve the yield is the primary problem in the study of plant polysaccharides. [0003] At present, the area of ​​camellia oleifera in my country is about 45 million mu. The national annual output of camellia oleifera seeds is about 1 million tons, and 683,900 tons of camel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06
Inventor 李湘洲张胜吴志平林科峰
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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