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Method for synchronously extracting vegetable seed oil and protein

A technology of simultaneous extraction and rapeseed oil, applied in the field of simultaneous extraction of rapeseed oil and protein, to achieve the effects of safe operation, high protein yield and simple equipment

Inactive Publication Date: 2012-11-28
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no process that can simultaneously extract rapeseed oil and rapeseed protein

Method used

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  • Method for synchronously extracting vegetable seed oil and protein

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Weigh 100 g of peeled and crushed rapeseed and add 500 ml of distilled water to mix to obtain a mixed solution, boil the mixed solution for 5 minutes, adjust the pH of the mixed solution to 5.0 after cooling, add complex polysaccharide enzymes (pectinase, cellulase and β-glucan Enzyme compounded at 4:1:1) Hydrolyzed at 45°C for 2 hours to obtain enzymatic solution 1, adjust the pH of enzymatic solution 1 to 9.0, add protex-6L alkaline protease and enzymolyze at 55°C for 1 hour to obtain enzymatic solution 2. Centrifuge the enzymatic solution 2 to obtain hydrolyzate, emulsion, free oil 1 and residue; (2) Freeze the emulsion obtained in step (1) for 2 hours, heat at 90°C for 10 minutes, and then centrifuge to obtain free oil 2; ( 3) Collect the hydrolyzate obtained in step (1), add ethanol for ultrasonic extraction, the volume ratio of the added amount of ethanol to the hydrolyzate is 1:1, the ethanol concentration is 80%, the ultrasonic time is 20min, and centrifuged afte...

Embodiment 2

[0019] Weigh 100 g of peeled and crushed rapeseed and add 500 ml of distilled water to mix to obtain a mixed solution, boil the mixed solution for 5 minutes, adjust the pH of the mixed solution to 5.0 after cooling, add complex polysaccharide enzymes (pectinase, cellulase and β-glucan Enzyme compounded at 4:1:1) Hydrolyzed at 45°C for 2 hours to obtain enzymatic solution 1, adjust the pH of enzymatic solution 1 to 9.0, add protex-6L alkaline protease and enzymolyze at 55°C for 1 hour to obtain enzymatic solution 2. Centrifuge the enzymatic solution 2 to obtain hydrolyzate, emulsion, free oil 1 and residue; (2) Freeze the emulsion obtained in step (1) for 2 hours, heat at 90°C for 10 minutes, and then centrifuge to obtain free oil 2; ( 3) Collect the hydrolyzate obtained in step (1), add ethanol for ultrasonic extraction, the volume ratio of the added amount of ethanol to the hydrolyzate is 0.5:1, the ethanol concentration is 60%, the ultrasonic time is 30min, and centrifuged af...

Embodiment 3

[0021] Weigh 100 g of peeled and crushed rapeseed and add 500 ml of distilled water to mix to obtain a mixed solution, boil the mixed solution for 5 minutes, adjust the pH of the mixed solution to 5.0 after cooling, add complex polysaccharide enzymes (pectinase, cellulase and β-glucan Enzyme compounded at 4:1:1) Hydrolyzed at 45°C for 2 hours to obtain enzymatic solution 1, adjust the pH of enzymatic solution 1 to 9.0, add protex-6L alkaline protease and enzymolyze at 55°C for 1 hour to obtain enzymatic solution 2. Centrifuge the enzymatic solution 2 to obtain hydrolyzate, emulsion, free oil 1 and residue; (2) Freeze the emulsion obtained in step (1) for 2 hours, heat at 90°C for 10 minutes, and then centrifuge to obtain free oil 2; ( 3) Collect the hydrolyzate obtained in step (1), add ethanol for ultrasonic extraction, the volume ratio of the added amount of ethanol to the hydrolyzate is 0.5:1, the ethanol concentration is 60%, the ultrasonic time is 30min, and centrifuged af...

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Abstract

A method for synchronously extracting vegetable seed oil and protein belongs to vegetable fat extracting and processing technologies. The method comprises the following steps of: (1) mixing peeled vegetable seeds which are ground and water into a mixed liquor, boiling the mixed liquor, regulating the pH of the mixed liquor after being cooled, adding compound polysaccharidase for enzymolysis, regulating the pH after the enzymolysis, adding alkaline proteinase for continuous enzymolysis to obtain an enzymolysis liquor, centrifugally separating the enzymolysis liquor, and thus obtaining hydrolysate, emulsion, free oil 1 and residues; (2) freezing and then heating the emulsion which is obtained in the step (1), and then centrifuging to obtain free oil 2; and (3) collecting the hydrolysate which is obtained in the step (1), adding ethanol to carry out ultrasonic extraction, centrifugally separating after the extraction to obtain supernatant and precipitation, rotatably vaporizing the supernatant, centrifuging to obtain free oil 3, spray drying the precipitation to obtain vegetable seed protein, collecting the free oils 1, 2 and 3, and thus obtaining vegetable seed oil. The method can be used for synchronously obtaining high quality green vegetable seed oil and high purity vegetable seed protein, and simultaneously removes anti-nutritional factors which are harmful to the human body, the required equipment is simple, and is safe to operate, the obtained vegetable seed oil does not have residual solvent, and the oil recovery rate is high.

Description

technical field [0001] The invention belongs to the extraction and processing technology of vegetable oil, and mainly relates to a method for synchronously extracting rapeseed oil and protein. Background technique [0002] Traditional oil-making processes include pressing and direct extraction. The oil produced by the pressing method has a high residual oil rate, high labor intensity, high production cost, and large power consumption, and the solvent recovery system of the oil produced by the direct leaching method has a large load. The use of organic solvents increases the complexity of the process, reduces the safety of production, and causes environmental pollution. The traces of organic solvents remaining in the refined oil are not good for human health. Therefore, we are looking for a new "non-toxic and environmentally friendly" oil extraction technology. It has become a hot issue of concern all over the world. [0003] Rapeseed oil is a kind of nutrient-rich oil, the...

Claims

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Application Information

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IPC IPC(8): C11B1/10C11B1/04C11B1/00C07K1/30
Inventor 李杨江连洲齐宝坤冯红霞曹亮王心刚
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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