Detection kit for prostate specific antigen and preparation method thereof

Abstract

The invention relates to the field of immunoassay medicine, and specifically provides a chemiluminescence immunoassay diagnostic kit for a free prostate specific antigen (fPSA) and a preparation method thereof. The kit provided by the invention comprises an anti-FITC antibody coated solid phase vector, an FITC marked anti-PSA antibody, an enzyme marked anti-fPSA antibody, a chemiluminescent substrate acted by the above enzyme, a negative control solution and a positive control solution. The preparation method of the above kit comprises the following steps: 1) coating a solid-phase vector with the anti-FITC antibody; 2) marking the anti-PSA antibody with FITC to obtain the FITC marked anti-PSA antibody; 3) marking the anti-PSA antibody with an enzyme to obtain the enzyme marked anti-fPSA antibody; and 4) subpackaging and assembling. The kit provided by the invention has low cost and high sensitivity, and can improve sensitivity and reproducibility of the assay and reduce the usage amount of antibodies compared with an existing chemiluminescence immunoassay detection method.

Description

technical field

[0001] The invention relates to the field of immunoanalysis medicine, in particular, the invention provides a chemiluminescence immunoassay diagnostic kit for prostate specific antigen and a preparation method thereof. Background technique

[0002] With the aging of the population, changes in dietary structure and environmental factors, and the improvement of medical care, the incidence of prostate cancer is increasing year by year, ranking third among male urinary and reproductive system malignancies. Prostate cancer generally has no obvious symptoms in the early stage. Once it is discovered, it often appears in the late stage, and the best time for treatment is missed. Therefore, sensitive early diagnosis methods are needed.

[0003] Prostate-specific antigen (PSA, prostate specific antigen) is currently the most sensitive tumor marker for clinical diagnosis of prostate cancer. It has been recognized as an important indicator for the detection and evaluatio...