Method for producing biologic protein feed by using solid-state fermentation fiber dreg

A technology of biological protein feed and solid-state fermentation, applied in animal feed, animal feed, application and other directions, can solve the problems of low added value of products and low degree of degradable utilization of corn husk residue, and achieve the goal of increasing added value and reducing production costs. Effect

Active Publication Date: 2012-12-19
甘肃昆仑生化有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low degree of degradability and utilization of corn dregs, it is only sold cheaply as a common raw material for ruminant feed, and the added value of the product is low

Method used

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  • Method for producing biologic protein feed by using solid-state fermentation fiber dreg

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Experimental program
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Effect test

Embodiment 1

[0041] Inoculate Trichoderma lignin into a large test tube containing 5ml of wort after activation, culture on a shaker at 28°C with a rotation speed of 220r / min for 20 hours, and then inoculate 5% of the inoculum into a Erlenmeyer flask containing 500ml of wort , cultured on a shaking table at 28°C for 20h, inserted into the sterilized liquid strain fermenter and fermented at 28°C for 16h to obtain the first liquid strain; activated Candida utilis and Saccharomyces cerevisiae were inoculated into 5ml of wort together In a large test tube, culture on a shaker at 28°C with a rotation speed of 220r / min for 20h, then inoculate 5% of the inoculum into a conical flask containing 500ml of wort, cultivate on a shaker at 28°C for 20h, and insert it into another sterilized The liquid strain fermenter was fermented at 30° C. for 16 hours to obtain the second liquid strain. Get the corn soaking water produced in the soaking process in the wet corn starch production process, add 5g corn f...

Embodiment 2

[0047] Inoculate Trichoderma lignin into a large test tube containing 5ml of wort after activation, culture on a shaker at 28°C with a rotation speed of 220r / min for 20 hours, and then inoculate 5% of the inoculum into a Erlenmeyer flask containing 500ml of wort , cultured on a shaker at 28°C for 20h, inserted into the sterilized liquid strain fermenter and fermented at 30°C for 16h to obtain the first liquid strain; activated Candida utilis and Saccharomyces cerevisiae were inoculated into 5ml of wort together In a large test tube, culture on a shaker at 28°C with a rotation speed of 220r / min for 20h, then inoculate 5% of the inoculum into a conical flask containing 500ml of wort, cultivate on a shaker at 28°C for 20h, and insert it into another sterilized Ferment the liquid strain fermenter at 29°C for 16 hours to obtain the second liquid strain; take the corn soaking water produced in the soaking process in the wet corn starch production process, add 7.5g corn flour and 1.5g...

Embodiment 3

[0054] Inoculate Trichoderma lignin into a large test tube containing 5ml of wort after activation, culture on a shaker at 28°C with a rotation speed of 220r / min for 20 hours, and then inoculate 5% of the inoculum into a Erlenmeyer flask containing 500ml of wort , cultured on a shaker at 28°C for 20h, inserted into the sterilized liquid strain fermenter and fermented at 29°C for 16h to obtain the first liquid strain; activated Candida utilis and Saccharomyces cerevisiae were inoculated into 5ml of wort together In a large test tube, culture on a shaker at 28°C with a rotation speed of 220r / min for 20h, then inoculate 5% of the inoculum into a conical flask containing 500ml of wort, cultivate on a shaker at 28°C for 20h, and insert it into another sterilized The liquid strain fermentation tank was fermented at 29°C for 16 hours to obtain the second liquid strain; take the corn soaking water produced in the soaking process in the wet corn starch production process, add 6.5g corn ...

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Abstract

The invention provides a method for producing biologic protein feed by using solid-state fermentation fiber dreg. The method comprises the steps: activating, inoculating, cultivating and fermenting lignin trichodermin strain, candida utilis and saccharomyces cerevisiae to obtain a first liquid strain and a second liquid strain; adding maize flour, ammonium sulfate and potassium dihydrogen phosphate in maize soaking water, regulating pH value, conducting steam sterilization to obtain liquid nutrient solution; evenly agitating the dried wet maize peel dreg with bran, maize steep liquor, bean pulp, rice chaff and urea together, regulating pH value to obtain starting crude base stock; evenly mixing the liquid nutrient solution and the starting crude base stock, then inoculating, fermenting, continuously agitating and supplying fresh air, simultaneously adding inorganic salt, and evenly mixing; and drying the fermented materials at the temperature lower than 55 DEG C to obtain the biologicprotein feed. According to the method, the biologic protein feed is produced by utilizing wet maize peel dreg generated in process of producing starch from maize through wet method, and maize soakingwater, so that the additional value is increased, and the cost is lowered.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a production method of biological protein feed, in particular to a method for producing biological protein feed by biotechnological fermentation using corn soaking waste water and skin dregs produced from corn starch. Background technique [0002] A large amount of corn fiber dregs will be produced during the production of corn starch (generally 1 ton of corn produces 120-140kg of corn dregs with a moisture content of 2-10%). The test found that corn fiber husks contain a lot of cellulose, hemicellulose, pectin, and various nutrients such as starch, protein, vitamins and mineral elements. Due to the low degree of degradability and utilization of corn dregs, it is only sold cheaply as a common raw material for ruminant feed, and the added value of the product is low. [0003] The soaking process of wet corn starch production will produce a large amount of corn soaking water. Generally,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/00
CPCY02P60/87
Inventor 刘忠扣曹文毅田雪李永和常小兵张炜林张世轶张广
Owner 甘肃昆仑生化有限责任公司
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