Preparation method of jatrorrhizine

A kind of jatrorrhizine and preparation type technology, which is applied in the field of preparation of jatrorrhizine to achieve the effects of easy industrialization and short production cycle

Inactive Publication Date: 2012-12-19
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Easy to scale up for industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 200g of dried stems and leaves of Tendon, soak in 1% ammonia water for 4h, dry, add to flash extractor, use 90% ethanol solution as extraction solvent, extraction voltage 140V, extraction time 150s, flash Extract and filter to obtain the extract. The extract is decompressed to recover the reagent to obtain the extract. Add acid to adjust pH 3, filter out the insoluble matter, add alkali to adjust the pH of the filtrate to 9, enrich on D101 type macroporous resin column, wash with water until light color, then Elute with 35% ethanol solution, collect the eluate, concentrate and dry to obtain a crude product. Add the crude product to ethanol until it is just dissolved. The dissolved solution is filtered through a 0.45μm microfiltration membrane to obtain a sample solution. Take the filtrate for injection, use acetonitrile-0.3% phosphoric acid aqueous solution as the mobile phase, and the volume ratio is 25:75. The column is C8 The bonded phase is a filler, particle si...

Embodiment 2

[0020] Take 500g of dried stems and leaves of Tendon angustifolia, soak them in 1% ammonia water for 5h, dry them, add them to a flash extractor, use 90% ethanol solution as the extraction solvent, extract voltage 150V, extraction time 100s, flash Extract and filter to obtain the extract. The extract is decompressed to recover the reagent to obtain the extract. Add acid to adjust pH1, filter out the insoluble matter, add alkali to adjust the pH of the filtrate to 8, enrich on D101 type macroporous resin column, wash with water until light color, then Elute with 35% ethanol solution, collect the eluate, concentrate and dry to obtain a crude product. Add the crude product to ethanol until it is just dissolved. The dissolved solution is filtered through a 0.45μm microfiltration membrane to obtain a sample solution. Take the filtrate for injection, use acetonitrile-0.3% phosphoric acid aqueous solution as the mobile phase, and the volume ratio is 25:75. The column is C8 The bonded ...

Embodiment 3

[0022] Take 500g of dried stems and leaves of Tendon angustifolia, soak them in 1% ammonia water for 2h, dry them, add them to a flash extractor, use 85% ethanol solution as extraction solvent, extract voltage 140V, extraction time 120s, flash Extract and filter to obtain the extract. The extract is decompressed to recover the reagent to obtain the extract. Add acid to adjust pH2, filter out the insoluble matter, add alkali to adjust the pH of the filtrate to 8, enrich on the D101 type macroporous resin column, wash with water until light color, and then Elute with 35% ethanol solution, collect the eluate, concentrate and dry to obtain a crude product. Add the crude product to ethanol until it is just dissolved. The dissolved solution is filtered through a 0.45μm microfiltration membrane to obtain a sample solution. Take the filtrate for injection, use acetonitrile-0.3% phosphoric acid aqueous solution as the mobile phase, and the volume ratio is 25:75. The column is C8 The bon...

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PUM

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Abstract

The invention relates to a preparation method of jatrorrhizine. The preparation method comprises the following steps of: (1) taking dry stem leaves of mahonia fortunei (Lindl.) Fedde, soaking the stem leaves with 1% of saturated ammonia water for 2-6 hours, drying the stem leaves in the air, adding the stem leaves into a flash extractor, taking 85-95% of ethanol solution as an extraction solvent to carry out flash extraction, and filtering to obtain extracting solution; (2) concentrating the extracting solution to extract, adjusting the pH value to 1-3, filtering out insoluble substances, adjusting the pH value of the filtrate to 8-9, enriching the filtrate on a macroporous resin column, firstly washing the product until the color is light, then eluting the product with 50% of ethanol solution, collecting the eluant, and concentrating and drying the eluant to obtain a crude product; and (3) separating the crude product through preparative liquid chromatography to obtain the jatrorrhizine. The method has the characteristics of quickness, safety, high efficiency, energy conservation, avoidance of damaging active ingredients and the like, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method of jatrorrhizine, in particular to a method for flash extraction of jatrorrhizine. Background technique [0002] Jatrorrhizine, molecular formula C 20 h 20 NO 4 , molecular weight 338.39. Jatrorrhizine is a plant of the genus Berberidaceae. Mahonia fortunei (Lindl.) A tetrahydroisoquinoline alkaloid compound extracted from the stems and leaves of Fedde. Experimental studies have shown that jatrorrhizine has physiological activities such as detoxification, sterilization, sedation, antiarrhythmia, hypoglycemia, hypotension and anticancer. [0003] The stems and leaves of the top ten contributions of narrow leaves contain berberine, jatrorrhizine, spikine, palmatine, berbamine, magnolialine and other ingredients, which have the effects of clearing deficiency heat, dampness, and detoxification, and are clinically used to treat Tuberculosis and hemoptysis, bone steaming and hot flashes, dizziness and tinni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D455/03
Inventor 刘东锋万冬梅
Owner NANJING ZELANG MEDICAL TECH
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