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Method for extracting nudaphantin

A technology of bamboo and grass, applied in the direction of organic chemistry, etc., can solve the problems of less bamboo and no bamboo, and achieve the effect of short time, favorable for large-scale production operation, and large processing capacity

Inactive Publication Date: 2012-12-19
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few studies on bamboo plantin, and there is no domestic report on the industrial method of preparing bamboo tree plant by continuous countercurrent extraction technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take 1 kg of bamboo grass root and crush it into coarse powder, put it into the extraction tank, add 3L of 60% ethanol, conduct continuous countercurrent ultrasonic extraction at 50°C for 60 minutes, the ultrasonic frequency is 30KHz, concentrate the extract until it has no alcohol smell, dilute with water, filter, and polymerize the filtrate. Amide resin column adsorption, first eluted with water, then eluted with 65% ethanol solution, collected eluent, dried at low temperature to obtain crude product, dissolved in methanol, injected into preparative high performance liquid chromatography, 26% acetonitrile solution was used as mobile phase, Ultraviolet on-line detection, the detection wavelength is 270nm, high concentration fractions are collected, concentrated by rotary evaporation, and dried at low temperature to obtain bamboo plantin with a content of 97.2%.

Embodiment 2

[0015] Take 1 kg of bamboo grass root and crush it into coarse powder, put it into the extraction tank, add 70% ethanol, and conduct continuous countercurrent ultrasonic extraction at 50°C for 80 minutes at a temperature of 40KHz. Amide resin column adsorption, first eluted with water, then eluted with 60% ethanol solution, collected eluent, dried at low temperature to obtain crude product, dissolved in methanol, injected into preparative high performance liquid chromatography, 30% acetonitrile solution was used as mobile phase, Ultraviolet on-line detection, the detection wavelength is 270nm, high concentration fractions are collected, concentrated by rotary evaporation, and dried at low temperature to obtain bamboo plantin with a content of 95.2%.

Embodiment 3

[0017] Take 1 kg of bamboo grass root and crush it into coarse powder, put it into the extraction tank, add 75% ethanol, and conduct continuous countercurrent ultrasonic extraction at 30°C for 30 minutes, the ultrasonic frequency is 50KHz, concentrate the extract until it has no alcohol smell, dilute with water, filter, and polymerize the filtrate. Amide resin column adsorption, first eluted with water, then eluted with 55% ethanol solution, collected eluent, dried at low temperature to obtain crude product, dissolved in methanol, injected into preparative high performance liquid chromatography, 30% acetonitrile solution was used as mobile phase, Ultraviolet on-line detection, the detection wavelength is 270nm, high concentration fractions are collected, concentrated by rotary evaporation, and dried at low temperature to obtain bamboo plantin with a content of 95.9%.

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Abstract

The invention belongs to the field of extracting and preparing active ingredients of traditional Chinese medicines, and relates to a method for extracting nudaphantin. The method comprises the steps that root of chrysopogon aciculatus is used as a raw material and subjected to continuous counter-current ultrasonic extraction, and the extract solution is subjected to polyamide resin column adsorption, preparative high performance liquid chromatography separation and other steps to obtain the nudaphantin. By the method, the production period is short, the obtained product has high purity, and industrialization production is easy to realize.

Description

technical field [0001] The invention belongs to the field of extraction of effective components of traditional Chinese medicines, and relates to a method for extracting bamboo shoots. Background technique [0002] Nudaphantin, yellow crystal, molecular formula C 22 h 22 o 8 , with a molecular weight of 414.41, is a flavonoid glycoside compound extracted and isolated from the rhizome of bamboo grass. Pharmacological studies have shown that bamboo plant has cytotoxic activity on KB cells. Bamboo grass is the whole herb of the Commelinaceae plant Bamboo Grass, which has the effects of clearing heat and detoxifying, diuretic, reducing swelling, and stopping bleeding; it is mainly used for sores, carbuncles, sore throat, hot dysentery, white turbidity, dysuria, traumatic bleeding, etc. disease. [0003] At present, there are few studies on bamboo plantin, and there is no domestic report on the industrial method of producing bamboo tree plant by continuous countercurrent extr...

Claims

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Application Information

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IPC IPC(8): C07D493/18
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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