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Method for extracting theaflavin and theanine from tea leaves

A technology of theanine and theaflavin, which is applied in the field of extracting theaflavin and theanine from tea leaves, can solve the problems of low theaflavin and high extraction cost, and achieve easy operation and control , high purification efficiency

Active Publication Date: 2012-12-19
广州市一杰医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0031] In summary, although the above methods can extract high-content theaflavins or theaflavins monomers, and also use microorganisms or enzymatic methods to convert tea polyphenols, the content of theaflavins in tea leaves is extremely low, and the content of theaflavins in black tea is only 0.5%, very expensive to extract

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Add 100 kg of fresh tea leaves immediately after picking and mince them while adding water. The total volume of water added is 300 L; Copper chloride solution to a final concentration of 25mmol / L, then use 0.01mol / L hydrochloric acid solution to adjust the pH of the solution to 5.5; feed oxygen into the solution, preferably without a large number of bubbles, and carry out the reaction with stirring 1-6h; After filtering and removing the slag, the filtrate is extracted with twice the volume of petroleum ether, and the solution containing petroleum ether is removed to obtain a solution I that does not contain petroleum ether; the solution I that does not contain petroleum ether is treated with 0.01mol / L hydrochloric acid solution Adjust the pH value to 7, apply AB-8 macroporous resin for adsorption, rinse the resin with water, and then elute with 25% ethanol to obtain eluent II, then elute with 75% ethanol to obtain eluent III ; Concentrate the eluate II to 1 / 3-1 / 5 of its...

Embodiment 2

[0059] Add 100 kg of fresh tea leaves immediately after picking and mince them while adding water. The total volume of Cladosporium superficialis fermentation broth added is 500 L; add 200 kg of crude tea polyphenols with polyphenols above 30% and stir to dissolve; then add magnesium chloride to the final concentration to 30mmol / L, add copper chloride solution to a final concentration of 25mmol / L, then adjust the pH of the solution to 5.5 with 0.01mol / L hydrochloric acid solution; suitable, and carry out stirring reaction for 1-6h; after filtering and removing residue, the filtrate is extracted with twice the volume of petroleum ether, and the solution containing petroleum ether is removed to obtain a solution I that does not contain petroleum ether; the solution I that does not contain petroleum ether is used Adjust the pH value to 7 with 0.01mol / L hydrochloric acid solution, apply AB-8 macroporous resin for adsorption, rinse the resin with water, and then elute with 25% ethan...

Embodiment 3

[0063] Add 200 kg of fresh tea leaves immediately after picking and mince them while adding water. The total volume of Cladosporium superficialis fermentation broth added is 800 L; add 500 kg of crude tea polyphenols with polyphenols above 30% and stir to dissolve; then add magnesium chloride to the final concentration to 30mmol / L, add copper chloride solution to a final concentration of 25mmol / L, then adjust the pH of the solution to 5.5 with 0.01mol / L hydrochloric acid solution; suitable, and carry out stirring reaction for 1-6h; after filtering and removing residue, the filtrate is extracted with twice the volume of petroleum ether, and the solution containing petroleum ether is removed to obtain a solution I that does not contain petroleum ether; the solution I that does not contain petroleum ether is used Adjust the pH value to 7 with 0.01mol / L hydrochloric acid solution, apply AB-8 macroporous resin for adsorption, rinse the resin with water, and then elute with 25% ethan...

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Abstract

The invention provides a method for extracting theaflavin and theanine from tea leaves. According to the method, water is added into fresh tea leaves, the tea leaves are ground, the ground tea leaves are dissolved through coarse tea polyphenol addition by adopting ultrafine cladosporium fermentation liquid for replacing water, then, magnesium chloride and copper chloride solution is added, the oxidation is carried out under the acid condition, the stirring is carried out, the ventilation reaction is taken, tea pigment using theaflavin and thearubigins as major ingredients is produced under the condition of enzymatic oxidation polymerization reaction, and then, filtering separation, purification, concentration and drying are carried out to obtain tea pigment and theanine.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and mainly relates to a method for extracting theaflavins and theanine, and more specifically relates to a method for extracting theaflavins and theanine from tea leaves. Background technique [0002] Tea is one of my country's important economic crops and export-earning products. It is mainly traditional rough-processed products. In recent years, the supply of rough tea products has exceeded demand. There is a backlog of about 100,000 tons of low-grade tea every year, which seriously hinders the development of my country's tea industry. healthy growth. [0003] As an emerging field of the tea industry, tea deep processing is conducive to the sustainable development of the tea industry, and has huge value-added potential and market demand. With the continuous deepening of tea deep processing research and the intersection and penetration of medical research, it has been confirmed...

Claims

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Application Information

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IPC IPC(8): C12P17/16C07C237/06C07C231/24C12R1/645
Inventor 李兰岚李光锋代洪波黄生有
Owner 广州市一杰医药科技有限公司
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