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Method for extracting and purifying tobacco sucrose tetraester

A technology of sucrose tetraester and tobacco, applied in the direction of chemical instruments and methods, esterified saccharides, sugar derivatives, etc., to achieve the effect of large processing capacity, high product purity and high purity

Inactive Publication Date: 2015-02-25
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the change of cigarettes to low tar and lower tar, the traditional flavoring technology and traditional flavoring and fragrance commonly used at present cannot meet the corresponding requirements, and there is an urgent need for new flavors and supporting technologies that meet the requirements of low-tar cigarette products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] A method for extracting and purifying tobacco sucrose tetraester, its steps:

[0012] (1) After crushing 50g of tobacco leaves, pass through a 20-mesh sieve, add 500g of dichloromethane, extract by ultrasonic wave for 20 minutes, and use 100g of methanol-water solution for liquid-liquid extraction. The mass ratio of methanol to water is 4:1. Obtain sucrose tetraester compound;

[0013] (2) Separation of sucrose tetraesters by silica gel column chromatography, the silica gel column chromatography (chromatographic column (60cm×4cm i.d.) uses neutral silica gel of 100 mesh, and wet packing adopts gradient elution method, First elute with n-hexane-acetone, the volume ratio of n-hexane and acetone is 1:1, the elution rate is 4ml / min, collect the eluate (40mL / bottle), and obtain the first group of fractions, the first group of fractions is 6 to 8 bottles, and then eluted with n-hexane-acetone, the volume ratio of n-hexane and acetone is 2:3, to obtain the second group of fra...

Embodiment 2

[0015] A method for extracting and purifying tobacco sucrose tetraester, its steps:

[0016] (1) After crushing 50g of tobacco leaves, pass through a 60-mesh sieve, add 1000g of dichloromethane, and conduct ultrasonic extraction for 40 minutes. The extract is subjected to liquid-liquid extraction with 150 g of methanol-water solution. The mass ratio of methanol to water is 6:1. Obtain sucrose tetraester compound;

[0017] (2) Separation of sucrose tetraesters by silica gel column chromatography, the silica gel column chromatography (chromatographic column (60cm×4cm i.d.) uses a neutral silica gel of 200 mesh, and the wet packing adopts gradient elution method, First elute with n-hexane-acetone, the volume ratio of n-hexane and acetone is 1:1, the elution rate is 4ml / min, collect the eluate (40mL / bottle), and obtain the first group of fractions, the first group of fractions is 6 to 8 bottles, and then eluted with n-hexane-acetone, the volume ratio of n-hexane and acetone is 2:...

Embodiment 3

[0019] A method for extracting and purifying tobacco sucrose tetraester, its steps:

[0020] (1) After crushing 50g of tobacco leaves, pass through a 40-mesh sieve, add 800g of dichloromethane, extract by ultrasonic wave for 30 minutes, and use 100g of methanol-water solution for liquid-liquid extraction, the mass ratio of methanol to water is 4:1, Obtain sucrose tetraester compound;

[0021] (2) Separation of sucrose tetraesters by silica gel column chromatography, the silica gel column chromatography (chromatographic column (60cm×4cm i.d.) uses a neutral silica gel of 160 mesh, and the wet packing adopts gradient elution method, First elute with n-hexane-acetone, the volume ratio of n-hexane and acetone is 1:1, the elution rate is 4ml / min, collect the eluent, and obtain the first group of fractions (40mL / bottle), the first group of fractions is 6 to 8 bottles, and then eluted with n-hexane-acetone, the volume ratio of n-hexane and acetone is 2:3, to obtain the second group ...

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PUM

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Abstract

The invention discloses a method for extracting and purifying tobacco sucrose tetraester. The method comprises the following steps of: (1) screening by a 20-60-mesh sieve after smashing tobacco leaves, adding dichloromethane, enabling a mass ratio of the tobacco leaves to the dichloromethane to be 1:10-20, extracting for 20-40 minutes through ultrasonic waves, conducting liquid-liquid extraction on extraction liquid by using a methanol-water solution, enabling a mass ratio of the carbinol to water to be 4-6:1, enabling a mass ratio of the tobacco leaves to the methanol-water solution to be 1:2-3, and obtaining a sucrose tetraester compound; and (2) separating the compound by using silicagel column chromatography and semi-prepared high performance liquid chromatography to obtain the sucrose tetraester. Purity of the sucrose tetraester obtained by using the method provided by the invention is higher than 98%, and an extraction ratio after purification is 0.1-0.3%.

Description

technical field [0001] The invention relates to a method for extracting and purifying tobacco sucrose tetraester. Background technique [0002] As we all know, cigarettes are a hobby product. With the substantial reduction of tar content, the original aroma quality of tobacco leaves is bound to be affected. Tar reduction and flavor preservation technology has become the key to restricting the development of low-tar cigarettes, especially in the current tar content. In the case of a large drop, it will become more and more difficult to further reduce the coke significantly while maintaining the smoking taste of cigarettes. How to reduce the amount of tar in cigarettes while still being able to accept the flavor of cigarettes has become the main subject of the cigarette industry, and it is also a key factor affecting the reduction of tar in cigarette products in my country. After ten years of research and development, my country's tobacco industry has generally mastered the t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H13/06C07H1/08
Inventor 毛多斌贾春晓杨靖王莹莹郑坚强
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY