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Vaccine used for preventing tilapia streptococcal disease

A technology for tilapia and streptococcus lactis, applied in the direction of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of high cost and complicated preparation methods, achieve high protection rate, simple preparation method, and good clinical application prospects Effect

Active Publication Date: 2013-01-02
TONGWEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The vaccines prepared by using Streptococcus agalactiae disclosed in the prior art are all double vaccines, the preparation method is complicated, the cost is high, and there are safety problems. It is necessary to find a vaccine that is safer and lower in cost

Method used

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  • Vaccine used for preventing tilapia streptococcal disease
  • Vaccine used for preventing tilapia streptococcal disease
  • Vaccine used for preventing tilapia streptococcal disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The isolation and identification of embodiment 1 bacterial strain

[0023] 1. Experimental materials and methods

[0024] 1.1 Experimental materials

[0025] The bacteria to be identified were isolated from the diseased tilapia in Wenchang, Hainan. The isolation medium was BHI, the culture temperature was 37°C, and the number was WR-6.

[0026] Reagent Consumables 16S rDNA Universal Amplification Kit (TAKARA), BHI Medium, Bacterial Biochemical Trace Identification Tube (Hangzhou Tianhe Biological Reagent Co., Ltd.), Taq DNA Polymerase (TAKARA), Agarose (TianGen), DL2000DNA maker (TianGen ).

[0027] 1.2 Experimental method

[0028] 1.2.1 Gram stain observation

[0029] Take a fresh slant cultivated overnight, pick a small amount of bacteria with an inoculation loop, apply it on a glass slide dripped with a small amount of sterile saline, perform Gram staining, and observe the staining results under a microscope.

[0030] 1.2. Amplification and analysis of 216S rDNA...

Embodiment 2

[0055] Example 2 Preparation of inactivated vaccine against Streptococcus agalactiae (WR-6) in tilapia and research on immune effect

[0056] 1. Materials and methods

[0057] 1.1 Materials

[0058] Tilapia Streptococcus agalactiae (WR-6) was isolated and identified from tilapia naturally infected with streptococcosis, and it was preserved in the China Center for Type Culture Collection with the preservation number CCTCC NO: M2011271.

[0059] Healthy tilapia were purchased from a farm in Hainan, with an average body weight of 237±8.6g, and were domesticated in an aquarium for 1 week before the experiment.

[0060] 1.2 Method

[0061] 1.2.1 Preparation of vaccine

[0062] Inoculate Streptococcus agalactiae on BHI slant medium and incubate at 37°C for 24 hours, pick colonies and inoculate them on BHI plate medium for 24 hours at 37°C, elute with sterile saline, adjust the concentration of bacteria to 1.0×10 8 CFU / ml, then add 0.6% formalin and inactivate by shaking at 37°C ...

Embodiment 3

[0077] Example 3 Comparative study on the immune effect of the inactivated whole-bacteria vaccine prepared by tilapia Streptococcus agalactiae (WR-6) and the standard strain of Streptococcus agalactiae (ATCC51487)

[0078] 1. Materials and methods

[0079] 1.1 Strains

[0080] Tilapia Streptococcus agalactiae (WR-6) isolated and identified from tilapia naturally infected with streptococcosis, preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2011271

[0081] The standard strain of Streptococcus agalactiae (ATCC51487) was donated by the Fish Disease Research Center of Sichuan Agricultural University

[0082] 1.2 Test fish

[0083] Healthy tilapia (Tilapia) purchased from a farm in Wenchang, Hainan, with an average body weight of 372±5.9g, were domesticated in an aquarium for 1 week before the experiment.

[0084] 1.3 Preparation of vaccine

[0085] Streptococcus agalactiae (WR-6) and the standard strain of Streptococcus agalacti...

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Abstract

The invention discloses a vaccine used for preventing tilapia streptococcal disease. The vaccine is prepared by using streptococcus agalactiae and pharmaceutically acceptable excipients or carriers. The invention discloses a purpose of the vaccine in preparing medicines used for preventing tilapia streptococcal disease, and a combinatively used medicine. The invention also discloses a novel streptococcus agalactiae strain. The vaccine provided by the invention can effectively stimulate tilapia to produce immunity resisting streptococcal disease. The vaccine has a high protection rate in preventing tilapia streptococcal disease, and has high practical value and good economic benefit.

Description

technical field [0001] The invention relates to a fish vaccine, in particular to a vaccine for preventing tilapia streptococcosis. Background technique [0002] Tilapia (Tilapia) is a small and medium-sized fish. It is a freshwater farmed fish cultivated by key scientific research in the world's aquaculture industry. It is also known as one of the main sources of animal protein in the future. It is native to Africa and belongs to the family Cichlidae. A tropical fish similar to perch. Tilapia is one of the important export-oriented economic fish in my country, and its healthy breeding is of great significance. [0003] However, in recent years, large-scale tilapia streptococcal disease outbreaks have occurred in major tilapia production areas such as Guangdong, Hainan, and Fujian, which has a wide range of effects and difficult disease control, which has brought huge economic losses to farmers. The diseased fish showed poor spirit and appetite, black body color, wandering ...

Claims

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Application Information

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IPC IPC(8): A61K39/09A61P31/04C12N1/20C12R1/46
Inventor 肖丹汪开毓阳涛
Owner TONGWEI
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