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Erythropoietin receptor modified electrode, preparation method and applications thereof

A technology of erythropoietin and modified electrodes, which is applied in the field of electrochemical detection, can solve the problems of low detection efficiency, long experimental separation time, poor specificity, etc., and achieve the effect of simple preparation method, stable performance and accurate screening

Active Publication Date: 2013-01-02
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Problems solved by technology

For a long time, the screening and detection of EPO and rhEPO have mostly been combined with mass spectrometry, isoelectric focusing, and gel electrophoresis. However, these detection methods have shortcomings such as long experimental separation time, low detection efficiency, and poor specificity. Requirements for rapid and accurate screening

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  • Erythropoietin receptor modified electrode, preparation method and applications thereof
  • Erythropoietin receptor modified electrode, preparation method and applications thereof
  • Erythropoietin receptor modified electrode, preparation method and applications thereof

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Embodiment Construction

[0030]In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0031] Main reagent used in the embodiment and instrument source are as follows: lithium hydroxide monohydrate LiOH·H 2 O, zinc acetate dihydrate Zn(Ac) 2 2H 2 O was purchased from Shanghai Sangon Bioengineering Co., Ltd., K 3 [Fe(CN) 6 ], K 4 [Fe(CN) 6 ] purchased from Chongqing Dongfang Reagent Factory, glassy carbon electrode, saturated calomel electrode, platinum electrode, 0.3 μm and 0.05 μm Al 2 o 3 Powder was purchased from Tianjin Aida Hengsheng Technology Development Co., Ltd., PBS powder was purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd., EPOR was purchased from Novus Biologicals, USA, and EPO and rhEPO standard products were purchased from Abnova, USA. The KQ-5200B ultrasonic cleaner is a product of Kunshan Ultraso...

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Abstract

The present invention discloses an erythropoietin receptor modified electrode, wherein an electrode having an erythropoietin receptor fixed on the surface through ZnO sol-gel is adopted as a glassy carbon electrode for element recognition, the modified electrode has characteristics of simple preparation method and stable performance, and a response current value of the modified electrode remains about 77% of the initial value after placing in a dark place for 50 days at a temperature of 4 DEG C. According to the present invention, the modified electrode is adopted as a working electrode, a platinum electrode is adopted as a counter electrode, a saturated calomel electrode is adopted as a reference electrode, and a phosphate buffer solution containing 2 mmol / L of K3Fe(CN)6]-K4[Fe(CN)6] is adopted as a test base solution to establish an electrochemical biosensor so as to rapidly, specifically and sensitively detect erythropoietin (EPO) and / or recombinant human erythropoietin (rhEPO), wherein a linear range is 5 pg / L-500 ng / L, a detection limit is as low as 0.5 pg / L, particularly EPO and rhEPO can be accurately screened according to the peak potential, and the electrochemical biosensor is applicable for low concentration EPO or rhEPO detection, and stimulant rhEPO detection in sport competition.

Description

technical field [0001] The invention belongs to the technical field of electrochemical detection, relates to a modified electrode and a preparation method thereof, and also relates to an electrochemical biosensor formed by using the modified electrode as a working electrode and a detection method thereof. Background technique [0002] Erythropoietin (EPO) is a glycoprotein hormone, a hematopoietic growth factor mainly produced by the kidney in the human body, and its physiological function is to promote the production and release of bone marrow red blood cells. Humans synthesized recombinant human erythropoietin (rhEPO) for the first time in 1985 using genetic engineering technology. Because it has dual functions of mitogen and differentiation factor, it can produce blood transfusion effect without blood transfusion, so that patients can avoid Viral infection and the risk of overdose have played an important role in the treatment of renal anemia. At the same time, rhEPO has...

Claims

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Application Information

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IPC IPC(8): G01N27/30
CPCG01N27/3275G01N27/3277G01N33/746G01N33/48714B05D3/002G01N27/3276
Inventor 王云霞张立群府伟灵
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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