Polysaccharide conjugation with detoxified E. COLI heat labile enterotoxin (LT) used as vaccine

A heat-resistant enterotoxin and Escherichia coli technology, applied in the direction of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve problems affecting immunogenicity and safety

Inactive Publication Date: 2013-01-02
DEV CENT FOR BIOTECHNOLOGY
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TT and DT, these two toxoid proteins are legal vaccines for infants/children;...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polysaccharide conjugation with detoxified E. COLI heat labile enterotoxin (LT) used as vaccine
  • Polysaccharide conjugation with detoxified E. COLI heat labile enterotoxin (LT) used as vaccine
  • Polysaccharide conjugation with detoxified E. COLI heat labile enterotoxin (LT) used as vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] It has now been found that the conjugated polysaccharide-LTS61K vaccine prepared according to the present invention can surprisingly induce higher polysaccharide-specific IgG antibody titers and greater bactericidal activity in serum than polysaccharides or polysaccharides mixed with LTS61K .

[0033] The list of abbreviations used in this article is as follows:

[0034] CFU: colony forming unit

[0035] ELISA: Enzyme-Linked Immunosorbent Assay

[0036] Hib: Haemophilus influenzae type b

[0037] IEF: Isoelectric Focusing

[0038] LT: heat-labile enterotoxin

[0039] MALLS: Multi-Angle Laser Scattering

[0040] OD: optical density

[0041] PNPS: pneumococcal polysaccharide

[0042] PRP: polyribosyl ribitol phosphate

[0043] PS: polysaccharide

[0044] SDS-PAGE: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis

[0045] SEC_HPLC: Size Exclusion High Pressure Liquid Chromatography

[0046] RI: reflectivity

[0047] The new carrier protein used in the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A detoxified recombinant E. coli heat-labile enterotoxin mutant, LTS61K, is employed as a carrier protein to conjugate polysaccharide. The LTS61K contains a mutated mature sub-unit A (LTA) that includes lysine at amino acid position 61 and a wild-type mature sub-unit B (LTB). Various types of bacterial capsular polysaccharide antigens were chemically conjugated with the LTS61K protein by a reductive amination reaction. The conjugated polysaccharide-LTS61K products were physically, chemically and biochemically identified as soluble form. Rabbits were immunized intramuscularly to determine the immunogenicity of conjugated vaccines by ELISA to detect anti- polysaccharide antigen IgG titers and serum bactericidal assay thereby determining the functional activity of the antibodies.; Study results show that conjugated polysaccharide-LTS61K vaccines induce higher polysaccharide-specific IgG titers and greater bactericidal activity in sera than that of polysaccharide alone or polysaccharide mixed with LTS61K. The presence of anti-LTS61K serum IgG antibody alleviates travel diarrhea caused by E. coli (ETEC enterotoxigenic E. coli).

Description

[0001] Related Application Cross Reference [0002] This application claims priority to US Provisional Patent Application No. 61 / 330,650, filed May 3, 2010, the disclosure of which is incorporated herein by reference in its entirety. technical field Background technique [0003] Polysaccharide vaccines lack immune memory responses when prepared without the use of carrier proteins. Currently known conjugate vaccines include capsular polysaccharides from bacteria such as Haemophilus influenzae type b, Neisseria meningitidis group C, and Streptococcus pneumoniae serotypes 1, 3, 4 , 5, 6B, 7F, 9V, 14, 19F, 23F. The vaccine is covalently coupled to a carrier protein such as tetanus toxoid, diphtheria toxoid, CRM197, mutant avirulent diphtheria toxin, or N. meningitidis outer membrane protein. These polysaccharide-conjugated vaccines induce T-cell-dependent responses especially in infants younger than two years of age; and they promote long-term immune memory, produce high-affi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/385A61K39/38A61K39/108A61K39/02C12N1/20A61P1/12A61P31/04
CPCA61K2039/6037A61K39/385A61K47/4833A61K39/102A61K39/092A61K2039/55505A61K47/48261A61K47/6415A61K47/646A61P1/12A61P31/04
Inventor 徐悠深郭怡玲洪国展吕元馨阮大同
Owner DEV CENT FOR BIOTECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap