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Method for producing L-lactic acid and Exiguobacterium aurantiacum special for method

A microbacterium, golden orange technology, applied in the field of L-lactic acid production, can solve the problems of bacterial strain toxicity and failure to achieve the desired effect, and achieve the effect of reducing purification costs and reducing environmental pollution

Active Publication Date: 2013-10-23
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the toxicity of sodium ions to the strains, the current L-lactic acid fermentation using sodium hydroxide as a neutralizing agent at home and abroad has failed to achieve the desired effect

Method used

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  • Method for producing L-lactic acid and Exiguobacterium aurantiacum special for method
  • Method for producing L-lactic acid and Exiguobacterium aurantiacum special for method
  • Method for producing L-lactic acid and Exiguobacterium aurantiacum special for method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Isolation, screening and identification of Exiguobacterium aurantiacum 8-11-1

[0040] The culture medium used in this embodiment consists of the following:

[0041] Screening medium (g / L): glucose 20.0, yeast powder 5.0, polypeptone 5.0, K 2 HPO 4 ·3H 2 O 1.0, MgSO 4 ·7H 2 O 0.2, NaCl 50, distilled water 900ml, high temperature sterilization at 115°C for 10 minutes, then add high temperature sterilized 10% (w / v) Na 2 CO 3 100ml, pH 10.0.

[0042] Solid medium: add 15g / L agar on the basis of screening medium, pH is 10.0.

[0043] 1. Isolation and screening of Exiguobacterium aurantiacum 8-11-1

[0044] Water samples were obtained from the edge of the Ordos Saline-Alkali Lake in Inner Mongolia. Take 4ml of the sample and inoculate it into a 100ml Erlenmeyer flask containing 50ml of screening medium, activate and culture it at 37°C for 3 days, mix it well, take the sample and dilute it 10,000 times with sterile water, spread it on the solid medium, cul...

Embodiment 2

[0058] Example 2. Determination of the optimum reaction temperature for producing L-lactic acid by fermentation of Exiguobacterium aurantiacum 8-11-1CGMCC No.6154 using glucose as a carbon source (erlenmeyer flask)

[0059] The composition of medium used in the present embodiment is as follows:

[0060] Liquid medium (g / L): glucose 20.0, yeast powder 5.0, polypeptone 5.0, K 2 HPO 4 ·3H 2 O 1.0, MgSO 4 ·7H 2 O 0.2, NaCl 50, distilled water 900ml, high temperature sterilization at 115°C for 10 minutes, then add high temperature sterilized 10% (w / v) Na 2 CO 3 100ml, the initial pH of the culture medium is 10.0.

[0061] Solid plate medium: Add 15 g / L of agar to the above liquid medium.

[0062] The method for producing L-lactic acid by fermentation method of the present invention comprises the following steps:

[0063] (1) Plate culture: Inoculate the strain of Exiguobacterium aurantiacum 8-11-1CGMCCNo.6154 on a solid plate medium and culture at 37°C for 24 hours;

[006...

Embodiment 3

[0068] Example 3. Acid production by Exiguobacterium aurantiacum 8-11-1CGMCC No.6154 under different NaCl concentrations (erlenmeyer flask)

[0069] The composition of medium used in the present embodiment is as follows:

[0070] Liquid medium (g / L): glucose 20.0, yeast powder 5.0, polypeptone 5.0, K 2 HPO 4 ·3H 2 O 1.0, MgSO 4 ·7H 2 O 0.2, NaCl 50, distilled water 900ml, high temperature sterilization at 115°C for 10 minutes, then add high temperature sterilized 10% (w / v) Na 2 CO 3 100ml, the initial pH of the culture medium is 10.0. .

[0071] Solid plate medium: Add 15 g / L of agar to the above liquid medium.

[0072] Fermentation medium (g / L): glucose 20.0, yeast powder 5, polypeptone 5.0, K 2 HPO 4 ·3H 2 O 1.0, MgSO 4 ·7H 2 O 0.2, NaCl 0-200, distilled water 900ml, high temperature sterilization at 115°C for 10 minutes, then add high temperature sterilized 10% (w / v) Na 2 CO 3 100ml l, the initial pH of the culture medium was 10.0.

[0073] The method for ...

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Abstract

The invention discloses a method for producing L-lactic acid and Exiguobacterium aurantiacum special for the method. Exiguobacterium aurantiacum is particularly Exiguobacterium aurantiacum 8-11-1, and the accession number in China General Microbiological Culture Collection Center is CGMCC No.6154. Exiguobacterium aurantiacum 8-11-1 CGMCC No.6154 has good sodium salt resistance, glucose is used as a substrate, a sodium hydroxide acid adjusting mode is adopted, Exiguobacterium aurantiacum is used for fermenting and producing L-lactic acid at the condition of 37 DEG C, the concentration of L-lactic acid achieves 133g / L, the optical purity is higher than 99.9%, and the conversion rate of glucose is higher than 93%. Exiguobacterium aurantiacum 8-11-1 CGMCC No.6154 can adopt the sodium hydroxide acid adjusting mode for fermenting and producing L-lactic acid which is high in concentration and optical purity, thereby being favorable for reduction of lactic acid purification cost, and reduction of environment pollution, and having important actual and industrial application valve.

Description

technical field [0001] The invention relates to a method for producing L-lactic acid and the special microbacterium aureus. Background technique [0002] Lactic acid, also known as dihydroxypropionic acid, is one of the three largest organic acids in the world. As a traditional multi-purpose fine chemical, lactic acid can be used as sour agent, fragrance, preservative, plant growth regulator, biodegradable material, drug and pesticide, etc., used in food, pharmaceutical, brewing, tanning, Textile, environmental protection and agriculture. The production of lactic acid by microbial fermentation has low production cost, high product safety, and can specifically obtain optically pure lactic acid. It is the main method for large-scale production of lactic acid. In the process of lactic acid fermentation, due to the continuous production of products, the acidity of the fermentation broth gradually increases, resulting in severe inhibition of bacterial growth and acid production...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/01
Inventor 王爱妍薛燕芬姜旭王丽敏于波马延和
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI