Blood separating gel and preparation method thereof

Abstract

The invention discloses a blood separating gel. The blood separating gel is prepared by uniformly mixing the following components in part by mass: 5-20 parts of SEBS, 20-60 parts of white oil, 15-60 parts of diethylhexyl phthalate, 1-20 parts of hydrophobic nano-silica and 0.5-5 parts of hydrophilic nano-silica, and the density of the separating gel is 1.030-1.075 g / cm<3> at 25 DEG C. The invention also provides a preparation method of the blood separating gel. The separating gel disclosed by the invention is stable, is convenient to use, has favorable thixotropy, is easy in centrifugation and good in separating effect, not only can bear irradiation sterilization of Gamma rays but also is insoluble in blood plasma or blood serum to interfere detection results, and causes less influence to the environment during medical waste incineration. The preparation method provided by the invention is simple, and is short in production period, high in efficiency, mature in process, easy to control and convenient to popularize.

Description

technical field [0001] The invention belongs to the technical field of separation gel for separating plasma or serum from blood samples and its preparation. Specifically, it relates to a method for automatically isolating blood cells when centrifuging to separate plasma or serum by utilizing the density difference of blood components. Separating gel with plasma, or blood clot and serum and its preparation method. Background technique [0002] In clinical medical testing, plasma or serum is often used for biochemical and immune testing of patient blood samples. After collecting blood in clinical collection containers such as vacuum blood collection tubes, use the density difference of blood components (the density of blood cells and blood clots is generally 1.08g / cm2) 3 , while the density of plasma and serum is generally 1.02g / cm 3 ), either from heparin-anticoagulated blood samples by centrifugation to separate blood cells and plasma, or from clotted blood samples by cent...

AI Technical Summary

Problems solved by technology

[0003] CN 1046036C, CN 101570637B and USP 5547577 respectively disclose several separation gels prepared from silicone polymers and other components. Although these separation gels can effectively isolate blood samples during centrifugation, they have the following problems: (1) It cannot be used for gamma-ray irradiation sterilization, because the silicone polymer will be cross-linked after sterilization, resulting in failure to change to a fluid state during centrifugation and failure, and non-sterile products may cause bacterial infections in patients when they are used clinically (2) High viscosity requires large centrifugal force and long centrifugation time, usually about 10 minutes, which is inconvenient to use. Excessive centrifugal force may also cause hemolysis, which will affect the test results; (3) Silicone The stability of the separation gel system is poor, and partial failure often occurs after 6 months in actual use. After a long period of storage, it may require greater centrifugal force and longer centrifugation time to separate blood samples; (4) Silicone Small molecules are easy to form oil droplets on the surface of plasma or serum, and gradually accumulate and adhere to the sampling needle on the detection equipment during long-term use, which may easily cause the sampling needle to block and fail

Although the separation gel can be sterilized by gamma ray irradiation, but because it contains chlorinated paraffin, the chloride produced during the incineration of medical waste will cause great harm to the environment

In addition, the molecular weight of the polymer contained in the separating gel is low, and it is easy to flow when the temperature rises during storage and transportation, thus affecting the use of the product