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Method for extracting simulenoline from zanthoxylum simulans hance

A technology of wild pepper alcohol alkali and wild pepper, which is applied in the field of natural medicine and chemistry, can solve the problems of few reports on the chemical components of the stem bark of wild pepper, and achieve the effects of easy recycling and reuse, simple operation, and environmental protection

Inactive Publication Date: 2013-01-16
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been many reports on the research on this genus at home and abroad, but there are few reports on the chemical components of the stem bark of Zanthoxylum bungeanum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take wild pepper herbs and crush them into 40-60 meshes, weigh 10kg, add 50L95% ethanol for reflux extraction for 1 hour, filter the extract, add 30L95% ethanol for reflux extraction for 1 hour, filter the extract, then add 30L reflux extraction for 1 hour, Combine the extracts to recover ethanol to the extract, add 8L of 1% hydrochloric acid aqueous solution to dissolve, filter, add 2kg of silica gel desiccant to dry, add 5L of petroleum ether for ultrasonic extraction for 1 hour, filter out petroleum ether, and then add 4L of chloroform for ultrasonic extraction for 1 hour. Chloroform is recovered from the chloroform extract, and 1kg of silica gel (120-200) mesh is added to dry it, and 3kg of silica gel is packed into the column, and the mixed solution of chloroform and methanol is at a volume ratio of 20:1, 10:1, 5:1, 3:1, 1:1 Carry out gradient elution, thin-layer detection, collect high-concentration fractions to recover reagents, and dry to obtain the pure product ...

Embodiment 2

[0017] Take wild pepper herbs and crush them into 40-60 meshes, weigh 10kg, add 60L of 90% ethanol for reflux extraction for 1.5 hours, filter the extract, add 5L of 99% ethanol for reflux extraction for 1 hour, combine the extracts to recover ethanol to the extract, add 5L of 3% Dissolve hydrochloric acid aqueous solution, filter, add 3kg of silica gel desiccant to dry, add 6L of petroleum ether for ultrasonic extraction for 1 hour, filter out petroleum ether, add 5L of chloroform for ultrasonic extraction for 1 hour, recover chloroform from the chloroform extract, add 800g of silica gel (120- 200) mesh drying, and 2.5kg silica gel column packing, chloroform methanol mixed solution according to the volume ratio of 20:1, 10:1, 5:1, 3:1, 1:1 for gradient elution, TLC detection, collection of high Reagents were recovered from concentration fractions, and dried to obtain pure prickly ash, with a content of 93.5%.

Embodiment 3

[0019] Take wild pepper herbs and crush them into 40-60 meshes, weigh 20kg, add 160L80% ethanol for reflux extraction for 2 hours, filter the extract, add 30L90% ethanol for reflux extraction for 1.5 hours, combine the extracts to recover ethanol to the extract, add 10L2% Dissolve hydrochloric acid aqueous solution, filter, add 8kg silica gel desiccant to dry, add 8L petroleum ether for ultrasonic extraction for 1 hour, filter out petroleum ether, add 6L chloroform for ultrasonic extraction for 1 hour, recover chloroform from chloroform extract, add silica gel 1.2kg (120 -200) mesh drying, and 5kg silica gel column packing, chloroform methanol mixed solution according to the volume ratio of 20:1, 10:1, 5:1, 3:1, 1:1 for gradient elution, TLC detection, collection of high Reagents were recovered from concentration fractions, and dried to obtain pure prickly ash, with a content of 92.7%.

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Abstract

The invention relates to a method for extracting simulenoline from zanthoxylum simulans hance. The method comprises the following steps of: crushing zanthoxylum simulans hance barks to form 40 to 60-mesh powder, adding the powder into 80 to 95 volume percent of ethanol aqueous solution in an amount which is 5 to 8 times that of the powder, performing reflux and extracting for 2 to 3 times, and recovering ethanol from an extracting solution under reduced pressure; adding an extract into a hydrochloric acid aqueous solution for dissolving, filtering, adding a silica-gel desiccant for drying, and performing ultrasonic extraction by using petroleum ether and chloroform sequentially; recovering the chloroform from a chloroform extracting solution, adding silica gel, mixing uniformly, drying, and packing the mixture; and eluting a mixed solution of the chloroform and methanol, performing thin-layer detection, collecting high-concentration fractions, recovering a reagent, and drying to obtain a pure simulenoline product. The method for producing the simulenoline is high in stability and specificity, and the pollution to environment is reduced.

Description

Technical field [0001] The present invention is a natural medicinal field, especially the method involving the extraction of wild peppercorns from wild peppercorns. Background technique [0002] Simulenoline is a kind of pyrine -like alkaline, molecular C 20 H 23 NO 3 , Molecular weight 325.41, colorless oily.Pharmacological studies have shown that wild pepper alcoholic alcohol has anti -platelet aggregation activity, which inhibit the aggregation of rabbit platelets induced by peanutic acid, collagen, and PAF. At 100 μg / ml, it completely inhibits collagen -induced platelet aggregation. [0003] Wild pepper Zanthoxylum Simulans Hance is a plant of Zanthoxylum Linn (Zanthoxylum Linn), also known as soil peppercorns, rock peppers, bamboo leaf pepper, mainly distributed in the south of the Yangtze River, Henan, Hebei and other places.Medicinal.This genus plant resources are relatively abundant. There are about 250 species in the world. It is distributed in tropical and subtropical r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/052
CPCY02P20/582
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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