Construction method of electrochemical sensor for dna single strand and protein molecular detection
A molecular detection and construction method technology, applied in the field of analysis and detection, can solve the problems of low sensitivity, high detection limit concentration, low signal value of electrochemical sensor, etc., achieve high sensitivity, low detection limit concentration, and improve detection sensitivity. Effect
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Embodiment 1
[0030] Such as figure 1 As shown, a gold disk electrode 110 with a diameter of 2.0 mm was taken, polished and polished with aluminum oxide powders with particle sizes of 1.0 mm, 0.3 mm, and 0.05 mm, and then the gold disk electrode 110 was successively placed on two Ultrasonic cleaning in water, ethanol, and acetone several times. The washed gold plate electrode 110 was put into 0.5M sodium hydroxide solution and 0.5M sulfuric acid solution successively for electrochemical cycle scanning, washed with secondary water for several times, and then the gold plate electrode 110 was blown dry with high-purity nitrogen gas. The treated gold disk electrode 110 was mixed with 0.5 μM capture probe 120 and 5.0 μM tris(2-carbonyl Ethyl) phosphate hydrochloride is reacted, and the reaction temperature is 37 ℃, and the duration is 16 hours, wherein the DNA sequence of capture probe 120 is: 5'-HS-(CH 2 ) 6 -AGA CAAGGA AAA TCC TTC CCC CCC CTG AAG TGG GTC GAA AA-3'. Under the same buffer so...
Embodiment 2
[0034] Such as Figure 4 As shown, the pretreatment process of the gold disc electrode 210 is the same as that of Embodiment 1. The treated gold disk electrode 210 reacts with 0.5 μM capture probe 220 and 5.0 μM tris (2-carbonylethyl) phosphate hydrochloride in a pH 7.4 concentration of 200 mM Tris-HCl buffer solution, and the reaction temperature is 37°C, the duration is 16 hours, wherein the capture probe 220 is the nucleic acid aptamer sequence of thrombin: 5'-HS-(CH 2 ) 6 -AGACAA GGA AAA TCC TTC CCC CCC CGG TTG GTG TGG TTG G-3'. Under the same buffer solution and reaction conditions, the gold disc electrode 210 was further reacted with 0.5 μM additional probe 230, the DNA sequence of the additional probe 230 was: 5′-GGT TGG TGT GGT TGG-(CH 2 ) 3 -SH-3'. After the gold disk electrode 210 modified by the capture probe 220 and the additional probe 230 is washed with a Tris-HCl buffer solution, it is further reacted with 6-mercaptoethanol in a 10 mM Tris-HCl buffer soluti...
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