Algicidal bacteria and method for removing microcystis aeruginosa

A technology of Microcystis aeruginosa and algae-dissolving bacteria, applied in the direction of microorganism-based methods, bacteria, chemical instruments and methods, etc., can solve problems such as no effective solutions, cyanobacteria bloom pollution, etc., and achieve low cost and high application promising effect

Active Publication Date: 2014-12-03
ANHUI HUANGHE WATER RESOURCE POLYTRON TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the outbreak of cyanobacteria is not limited to urban lakes, large-scale natural water bodies such as Taihu Lake, Dianchi Lake, Chaohu Lake, etc. are worthy of research, and the dominant species of cyanobacteria blooms is Microcystis aeruginosa. There are certain limitations in the removal of Microcystis aeruginosa by alginolytic bacteria, and there is no effective solution to the problem of cyanobacteria bloom pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] In this example, 100 mL of fresh Microcystis aeruginosa liquid 1 was treated, and the concentration of chlorophyll a was 91.72 mg / m 3 , pH value is 7.2. The specific implementation steps are as follows: first, culture Bacillus lysinus at a temperature of 30°C and a shaker speed of 130 r / min until the logarithmic phase (16 h), and then, according to the bacteria-to-algae ratio of 1:2, the 50 mL of the logarithmic phase bacterial liquid was inoculated into the algae liquid, and sterile water was used as a blank control, and the initial chlorophyll a concentration was determined to be 61.2 mg / m 3 , placed in a light incubator at 28°C, light intensity 2500 lux, and light cycle 12 h: 12 h for static culture, and samples were taken every 24 h to determine the concentration of chlorophyll a. After the algae liquid treated by the above method, the concentration of chlorophyll a was 34.2 mg / m at 96 h 3 , the removal rate was 44.12%, which can dissolve algae cells well.

Embodiment 2

[0049] What this embodiment deals with is 100 mL of fresh Microcystis aeruginosa liquid 2, and the concentration of chlorophyll a is 74.76 mg / m 3 , pH value is 7.2. The specific implementation steps are as follows: firstly, the Bacillus lysinica was cultured to the logarithmic phase (16 h) at a temperature of 30 °C and a shaker speed of 130 r / min, and then the bacteria were injected at a bacterial ratio of 1:5. 20 mL of logarithmic phase bacterial liquid was inoculated into the algae liquid, and sterile water was used as a blank control, and the initial chlorophyll a concentration was determined to be 62.3 mg / m 3 , placed in a light incubator at 28°C, light intensity 2500 lux, and light cycle 12 h: 12 h for static culture, and samples were taken every 24 h to determine the concentration of chlorophyll a. After the algae liquid treated by the above method, the concentration of chlorophyll a was 40.64 mg / m at 96 h 3 , the removal rate reached 34.77%, and the algae-dissolving e...

Embodiment 3

[0051] In this example, 100 mL of fresh Microcystis aeruginosa liquid 3 was treated, and the concentration of chlorophyll a was 71.17 mg / m 3 , pH value is 7.2. The specific implementation steps are as follows: First, culture Bacillus lysinus at a temperature of 30°C and a shaker speed of 130 r / min until the logarithmic phase (16 h), and then, according to the bacteria-to-algae ratio of 1:10, the 10 mL of the logarithmic phase bacterial liquid was inoculated into the algae liquid, and sterile water was used as a blank control, and the initial chlorophyll a concentration was determined to be 64.71 mg / m 3 , placed in a light incubator at 28°C, light intensity 2500 lux, and light cycle 12 h: 12 h for static culture, and samples were taken every 24 h to determine the concentration of chlorophyll a. After the algae liquid treated by the above method, the concentration of chlorophyll a was 51.45 mg / m at 96 h 3 , The removal rate is up to 20.49%. At this time, the algae-dissolving e...

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Abstract

The invention provides algicidal bacteria and a method for removing microcystis aeruginosa, and belongs to the technical field of environment-friendly water treatment. The invention provides lysinibacillusfusiformis which is stored with a number of CGMCCNO.6106. The invention also provides the method for degrading the microcystis aeruginosa by utilizing the lysinibacillusfusiformis. Screened algicidal bacteria provided by the invention has a certain effect of dissolving dominant algaes microcystis aeruginosa, and has no sensitivity to a light period; am algicidal characteristic is nearly not influenced by the light period, and cannot fluctuated along with variation of the light period; through the characteristic, the bacterium can better perform the algicidal characteristic in natural water; and a wider application prospect is provided for treating pollution of large-scale cyanobacterial bloom pollution in water.

Description

technical field [0001] The invention belongs to the technical field of environmental protection water treatment, and in particular relates to an algicidal bacterium screened from the intestinal tract of wild silver carp in the Taihu Lake Basin and a method for removing Microcystis aeruginosa by using the same. Background technique [0002] With the development of the economy and the progress of the society, people's requirements for the quality of life are increasing, and more and more sewage is caused by production and life. In the economically developed Yangtze River Delta region, a large amount of waste water containing pollutants such as nitrogen and phosphorus is Discharging into nearby natural water bodies will lead to frequent outbreaks of blue-green algae in rivers, lakes and other water bodies, seriously destroying the ecological environment of water bodies, threatening the survival of aquatic organisms, and affecting the use of water resources by humans. [0003] A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 张文艺陈雪珍李秋艳韩有法李仁霞
Owner ANHUI HUANGHE WATER RESOURCE POLYTRON TECH INC
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