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Method for supercritical CO2 extraction of effective ingredients of Haematococcus pluvialis spore powder

A technology of Haematococcus pluvialis and active ingredients, applied in organic chemistry, bulk chemical production, etc., can solve the problems of failure to comprehensively consider the correlation of various factors, lack of in-depth research on the purification process, and increase the difficulty of astaxanthin extraction, etc. Problems, to achieve the effect of enhancing carrying capacity, improving permeability and diffusion rate, and low production cost

Inactive Publication Date: 2013-02-06
JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECH
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Problems solved by technology

[0005] In recent years, the use of supercritical CO 2 Studies on the extraction of natural astaxanthin by fluid extraction have been reported one after another, and it is believed that high pressure, high temperature and low flow rate are beneficial to the extraction of natural astaxanthin, such as application number 200910102291.4, a method for extracting astaxanthin from Haematococcus pluvialis; application number : 201010548303.9 A preparation method of Haematococcus pluvialis extract, etc. Although these reports have done some research on the extraction process of astaxanthin, the relevant research basically stays on single factor research, without comprehensive consideration of the correlation between various factors In addition, there is no in-depth study on the pre-treatment of Haematococcus pluvialis spore powder, which affects its extraction efficiency, and its purification process after affecting the quality of its finished product.
Under certain adversity conditions, Haematococcus pluvialis synthesized and accumulated a large amount of astaxanthin, resulting in thicker cell walls and the presence of colloids, which increased the difficulty of extracting astaxanthin. At the same time, most of the astaxanthin in algae is It exists in the form of esters. In fact, the purity of astaxanthin extracted from algae is poor. This extract is not real astaxanthin, but a mixture of astaxanthin esters. Usually, the conversion rate of astaxanthin can only reach About 80%; and the extraction rate of astaxanthin extracted by supercritical CO2 extraction technology in the prior art is low, usually only about 7%

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  • Method for supercritical CO2 extraction of effective ingredients of Haematococcus pluvialis spore powder
  • Method for supercritical CO2 extraction of effective ingredients of Haematococcus pluvialis spore powder
  • Method for supercritical CO2 extraction of effective ingredients of Haematococcus pluvialis spore powder

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Experimental program
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Embodiment 1

[0041] The astaxanthin content weight percent is 1.5% Haematococcus pluvialis spore powder, frozen and crushed with low-temperature liquid nitrogen, the wall-breaking rate is 92%, the particle size is 100 mesh, granulated, and the broken wall of Haematococcus pluvialls The spore powder is subjected to physical characteristics wet granulation with alcohol, the particle size is 14-20 mesh, and then granulated, sieved, infiltrated, and the granules made of Haematococcus pluvialis spore powder are granulated with 95% edible alcohol. Soak, the granule that Haematococcus pluvialis spore powder makes: Edible alcohol=1: 1 is by mass ratio, soaking time is 4 hours; After 1000g glass beads are dried, mix evenly with 4000g Haematococcus pluvialls spore powder granule and carry out The grid filling and filling the kettle, the mixed grid filling adopts manual methods, the filling process should pay attention to the tightness of the material structure, and after the filling is completed, pre...

example 2

[0043] Take Haematococcus Pluvialls spore powder with astaxanthin content of 1.5%, freeze and pulverize the wall with low-temperature liquid nitrogen, the wall-breaking rate is 96%, and the particle size is 400 meshes, granulate, and the Haematococcus pluvialls spore powder after the wall is broken Wet granulation of physical properties by alcohol, granule size 16 mesh, granulation, sieving, infiltration, using edible alcohol with a mass percentage concentration of 95% to soak the granules made of Haematococcus pluvialis spore powder, Pluvialls red balls Granules made from algae spore powder: edible alcohol=1:10 by mass ratio, soaking time is 8 hours. After drying, 1000g of glass beads is mixed with 4000g of Haematococcus pluvialis spore powder particles evenly, and then filled into the kettle by gridding. The mixed gridding filling adopts manual method. Pay attention to the elastic structure of the material during the filling process. After filling, press supercritical CO 2...

example 3

[0045] Take Haematococcus pluvialis spore powder with astaxanthin content of 1.5%, freeze and pulverize the wall with low temperature liquid nitrogen, the wall breaking rate is 94%, the particle size is 150 mesh, granulate and granulate, and the broken wall of Haematococcus pluvialls The spore powder is wet granulated with physical properties by alcohol, the particle size is 20 mesh, sieved, infiltrated, and the granules made of Haematococcus pluvialis spore powder are soaked in edible alcohol with a mass percentage concentration of 95%. The granule that spore powder is made: edible alcohol=1: 5 is by mass ratio, soaking time is 6 hours, after 1000g glass beads are dried, mix with 4000g Haematococcus pluvialis spore powder granule and carry out gridding filling filling kettle, mix Mesh filling is done manually. Pay attention to the elastic structure of the material during the filling process. After filling, press supercritical CO 2 Fluid extraction operating procedures for f...

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Abstract

The invention discloses a method for supercritical CO2 extraction of effective ingredients of Haematococcus pluvialis spore powder, comprising the steps of: carrying out liquid-nitrogen low-temperature grinding physical wall breaking, granulation, infiltrate and other biological treatments on Haematococcus pluvialis spore powder according to the characteristic that the structure of astaxanthin in Haematococcus pluvialis is mainly 3S-3'S type, carrying out extraction separation with supercritical CO2 fluid, collecting astaxanthin extract by adjusting the temperature, pressure and flow of the fluid as well as extraction time in the extraction separation process, and carrying out saponification and liquid chromatography separation and purification to prepare high-grade astaxanthin, wherein the conversion rate of astaxanthin achieves about 90% and the extraction rate of astaxanthin is greatly increased.

Description

technical field [0001] The invention belongs to the technical method of extracting and refining and purifying natural biological resources, and mainly relates to supercritical CO 2 Method for fluid extraction of astaxanthin. Background technique [0002] A large number of studies have shown that the accumulation rate and total production of astaxanthin in Haematococcus pluvialis are higher than those of other green algae. The structure of astaxanthin in Haematococcus pluvialis is mainly 3S-3′S type. Coccus algae is recognized as the best organism in nature to produce natural astaxanthin. Therefore, the use of this microalgae to extract astaxanthin undoubtedly has broad development prospects, and has become a research hotspot in the world in recent years for the production of natural astaxanthin. Haematococcus pluvialis can rapidly synthesize and accumulate a large amount of astaxanthin under adversity stress conditions such as strong light, high temperature, nutrient (nitro...

Claims

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Application Information

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IPC IPC(8): C07C403/24
CPCY02P20/54
Inventor 毛日文邵兴军蒋云云
Owner JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECH
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