Production process of protease

Abstract

The present invention relates to a production process of protease, which comprises the following steps: 1) Seed cultivation: insert Bacillus licheniformis into slant culture medium and cultivate at 30-37°C for 24-30h under aerobic conditions, and then culture Put the good slant strains into the seed culture medium, and cultivate them on a constant temperature shaker at 30-37°C for 32-37h; then put the cultured strains into the primary seed culture and cultivate them at 31-38°C for 9.5-13h; 2) Fermentation: Insert the cultured primary seeds into fermentation culture based on 32-38°C for 31-35h; 3) Extract protease: transfer the cultured fermentation broth into a recovery tank and add diatomaceous earth and perlite to it, stir Uniform, obtained after solid-liquid separation, ultrafiltration membrane ultrafiltration treatment and spray drying. The yield of the process is high, and the quality of the obtained protease is good.

Description

technical field [0001] The invention belongs to the technical field of enzyme production, and in particular relates to a process for producing protease by using bacillus licheniformis. Background technique [0002] At present, the solid fermentation method with a relatively backward technical level is often used in China to produce protease, which has the disadvantages of high labor intensity, poor production environment conditions, and high bacterial contamination rate. Most of the products are solid crude enzymes, which can only be used for leather and silk degumming. A small number of industries, such as food, feed, and detergent, cannot meet the needs of the development of industries such as food, feed, and detergent. Therefore, food, feed, detergent and other industries that require high protease quality generally use imported products, which are expensive and increase the production cost of enterprises. Contents of the invention [0003] The purpose of the invention...

AI Technical Summary

Problems solved by technology

[0002] At present, the solid fermentation method with a relatively backward technical level is often used in China to produce protease, which has the disadvantages of high labor intensity, poor production environment conditions, and high bacterial contamination rate. Most of the products are solid crude enzymes, which can only be used for leather and silk degumming. A small number of industries, such as food, feed, and detergent, cannot meet the needs of the development of industries such as food, feed, and detergent.

Therefore, food, feed, detergent and other industries that require high protease quality generally use imported products, which are expensive and increase the production cost of enterprises.