Preparation method of zirconium group hydrophile chromatographic column and application thereof in measuring melamine content in raw milk or dairy product

A hydrophilic chromatographic column and melamine technology, which is applied in the field of chromatographic detection, can solve the problems affecting the sensitivity and accuracy of detection results, cumbersome sample pretreatment operations, and large amount of organic reagents, saving processing time, reducing noise, and standard deviation. small effect
CN102914599AInactive Publication Date: 2013-02-06HEBEI NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HEBEI NORMAL UNIV
Publication Date
2013-02-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a zirconium group hydrophile chromatographic column and an application thereof in measuring the melamine content in a raw milk or dairy product. Melamine in a sample is extracted in an ultrasonic, oscillating and centrifuging manner by using a 1% trichloroacetic acid-acetonitrile solution, and HPLC or LC-MS / MS segregation analysis is carried out on sample liquid filtered through a 0.2um filter membrane by using the zirconium group hydrophile chromatographic column. The method for measuring the melamine content in the raw milk or dairy product by using the chromatographic column is simple and convenient, rapid, sensitive and accurate, high in repeatability and low in detection cost. The separating mode is beneficial to the preparation of samples, so that the sample pretreatment is simple in operation, rapid, and high in recovery rate, has no interference on analysis and measurement, the samples can be treated in a batch manner, the time is saved, the measuring cost is reduced, the chromatographic column has long service life, and the indexes are superior to those by using a GB / T22388-2008 method. The method can be widely applied to detecting the melamine in the raw milk or dairy products.
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Description

technical field

[0001] The invention relates to a preparation method of a zirconium-based hydrophilic interaction chromatographic column and its application in determining the content of melamine in raw milk and dairy products, belonging to the technical field of chromatographic detection. Background technique

[0002] Columns are the "heart" of chromatography. An ideal chromatographic column should have good chemical stability and permeability; in addition, the column life of the chromatographic column should be long, so that the repeatability of chromatographic data can be fully guaranteed; the most important thing is that the chromatographic column should be suitable for the components to be measured. The retention effect, the capacity factor k' is preferably in the range of 2-10.

[0003] Melamine is a kind of chemical raw material. Because of the high nitrogen content in the molecule, it is called "protein essence". If melamine is contained in dairy products, it may c...

Claims

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