Method for detecting useful and harmful ingredients in flaxseed product

A technology of harmful components and detection methods, applied in the field of detection, to achieve the effect of offsetting errors and good reproducibility

Active Publication Date: 2013-02-06
内蒙古益善园生物科技有限责任公司
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Problems solved by technology

In recent years, there have been individual reports on the detection of cyanogenic glycosides in flaxseed by high performance liquid chromatography-mass spectrometry (HPLC-MS) [Park, Eun-Ryong; Hong, Jin-Hwan; Lee, Dong-Ha; Han, Sang-Bae; Lee, Kang-Bong; Park, Jae-Seok; Chung, Hyung-Wook; Hong, Kyung-Hyun; Kim, Myung-Chul. Analysis and decrease of cyanogenic glucosides in flaxseed. Korea Food and Drug Administration. (2005), 34 (6), 875-879], but there is no report using the more advantageous MRM mode detection method in the quantitative aspect
In addition, the simultaneous quantitative method of linseed lignan SDG, linalin and neolinalannin has not been reported yet.

Method used

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  • Method for detecting useful and harmful ingredients in flaxseed product
  • Method for detecting useful and harmful ingredients in flaxseed product
  • Method for detecting useful and harmful ingredients in flaxseed product

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Embodiment Construction

[0017] Such as image 3 The example and steps shown are only an example, but the internal standard compound is not limited to the compound used in the example, and other compounds with similar structures to the analyte can also be used as the internal standard, such as rutin and other phenolic components, trehalose, etc. other carbohydrates. One internal standard can be used, or two internal standard compounds can be used, one for SDG and one for cyanogenic glycosides. In the case of the same principle, the specific steps and the ratio of material to liquid for sample extraction can be adjusted appropriately.

[0018] ①Preparation of internal standard solution: prepare a methanol solution with a concentration of internal standard substance (abrusin-2″-O-apioside, Abrusin2″-0-β-apioside) of 10 μg / mL.

[0019] ② Prepare the solution for the standard curve: prepare a methanol solution containing 50 μg / mL lincyanin, neolincyanin and SDG and 10 μg / mL internal standard compound, a...

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Abstract

The invention discloses a method for detecting useful and harmful ingredients in a flaxseed product. The method comprises the main steps of: preparing an interior label solution containing an interior label compound with a certain concentration; preparing standard solutions (with a series of concentrations) of an ingredient to be detected by using the interior label solution; analyzing in an LC-MS MRM mode; drawing a standard curve, wherein an X-axle is used for representing the concentration and a Y-axle is used for representing the ratio of an ingredient peak area to an interior label peak area; extracting a flaxseed sample by using the interior label solution and carrying out LC-MS MRM mode analysis on the extracted solution; and calculating the content of the useful ingredient (secoisolariciresinol diglucoside and SDG) and the harmful ingredient (flax cyanogenic glycosides and new flax cyanogenic glycosides) in the flaxseed product by using the standard curve or a regression equation. The detection method has the advantages of speediness, accuracy, good reproducibility, short time and the like, and is an important monitoring means for the quality control and the production condition of the flaxseed product.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method of beneficial and harmful components in linseed products. Background technique [0002] Flax (linum usitatissimun L.), also known as flax, is one of the top ten oil crops in the world. Flaxseeds - Flaxseeds contain a variety of health-promoting components such as alpha-linolenic acid and lignans. Flaxseed lignan-secoisolariciresinol diglucoside (SDG) can improve women's menopausal symptoms and prevent breast cancer after in vivo metabolic activation [Zhang Caiyu, Zhang Bengang, Yang Xiuwei. Chemical constituents of flaxseed and its Advances in research on pharmacological effects. Chinese Journal of New Drugs, 2005, 14(5): 525-530]. On the other hand, flaxseed also contains cyanogenic glycosides-linocyanin and neolinocyanin. Cyanogenic glycosides will decompose and release highly toxic hydrocyanic acid under the action of enzymes or digestive tract acids and microorganis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 马超美王雅丽麻剑南刘治君刘强
Owner 内蒙古益善园生物科技有限责任公司
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