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Mycena dendrobii F48 compound microbial inoculant and application thereof

A technology of dendrobium small mushrooms and compound bacterial agents, applied in the fields of application, biocide, animal repellent, etc., can solve the problems of long time, slow plant growth and development, lack of endosperm tissue, etc., to increase the content of dendrobium and promote growth speed effect

Inactive Publication Date: 2013-02-13
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is that Dendrobium has a lot of flowers, and only a very small number of them can bear fruit. The fruit is a capsule, the seed is as fine as dust, the development of the embryo does not exceed the spherical stage, and it lacks endosperm tissue. The germination rate under natural conditions is less than 5%, and the growth and development of plants germinated from seeds are very slow
The second is that Dendrobium is almost all mycorrhizal plants. In the natural growth state, it must partially or completely rely on the establishment of a symbiotic relationship with fungi to complete normal life activities. The participation of root fungi makes it unable to grow normally, making it more difficult to industrialize the rare medicinal plant Dendrobium
In addition, it takes a long time for the tissue cultured seedlings of Dendrobium to flower, which is also one of the restrictive factors for tissue culture and reproduction. It usually takes 5 to 7 years for the tissue cultured seedlings to flower out of the bottle.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of the pure culture of dendrobium small mushroom F48

[0023] 1. Experimental materials: Dendrobium dendrobii F48 strain (Mycena dendrobii Fan et Guo) was isolated from wild Dendrobium nobile in Kunming, Yunnan.

[0024] 2. Liquid culture: Inoculate the F48 strain of Dendrobium chrysalis in PDA solid medium, culture it at 25°C for 14 days, cut out the F48 strain of Dendrobium chrysalis, and inoculate it in a sterile 500 mL triangle containing 100 mL of PD liquid medium. In the bottle, place it on a shaker at 28°C with a rotation speed of 200r / min to shake (reciprocate) for 10 days to obtain the seed liquid of Dendrobium chrysalis F48.

[0025] 3. Solid (3) fermentation culture: Inoculate the seed liquid obtained in step (2) into the solid fermentation medium at the ratio of 20mL seed liquid / 1000g fermentation medium, and ferment at 25°C for 20 days to obtain Dendrobium-containing mushroom F48 Solid bacterium block, the bacterium block is p...

Embodiment 2

[0026] Embodiment 2: the preparation of Trichoderma pure culture

[0027] 1. Experimental materials: Trichoderma was isolated from wild Dendrobium nobile in Kunming, Yunnan.

[0028] 2. Liquid culture: Trichoderma was inoculated into PDA solid medium, cultured at 25°C for 14 days, cut Trichoderma flakes, inoculated into a sterile 500 mL Erlenmeyer flask filled with 100 mL PD liquid medium, and placed in The seed liquid of Trichoderma was obtained by shaking (reciprocating) culture on a shaking table at 28°C and a rotation speed of 200r / min for 10 days.

[0029] 3. Solid (3) fermentation culture: Inoculate the seed solution obtained in step (2) into the solid fermentation medium at the ratio of 20mL seed solution / 1000g fermentation medium, and ferment at 25°C for 20 days to obtain the solid containing Trichoderma Bacteria block, the bacterium block is crushed to obtain the pure culture of the Dendrobium chrysalis F48; the final concentration of the solid fermentation medium is...

Embodiment 3

[0030] Embodiment 3: the preparation of dendrobium small mushroom F48 composite bacterial agent

[0031]Mix the pure culture of Dendrobium small mushroom F48 prepared in Example 1 and the pure culture of Trichoderma prepared in Example 2 according to the weight ratio=3.5:7.5, then add the total of the pure culture of Dendrobium small mushroom F48 and the pure culture of Trichoderma 2% by weight of Zhongxin 3A sticky powder (Liaoning Zhongxin Fishing Bait Development Center), mixed evenly to obtain Dendrobium small mushroom F48 compound bacterial agent.

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Abstract

The invention discloses a mycena dendrobii F48 compound microbial inoculant and application thereof. The mycena dendrobii F48 compound microbial inoculant is prepared from the following raw materials in parts by weight: 3.5 parts of mycena dendrobii F48 pure culture, 6.5 parts of trichoderma pure culture, and an adhering agent accounting for 2 percent of the total weight of the trichoderma pure culture and the mycena dendrobii F48 pure culture. The mycena dendrobii F48 compound microbial inoculant provided by the invention can be used for promoting the growth speed of a dendrobe seedling, increasing the contents of bioactivator polysaccharide and dendrobine and meeting the requirements of developing compound sustainable agriculture, ecological agriculture and green agriculture, and is suitable for large-scale production of the dendrobe as a rare medicinal plant.

Description

(1) Technical field [0001] The present invention relates to a composite microbial agent of Dendrobium safflower F48, in particular to a composite bacterial agent of Dendrobium small mushroom F48 (Mycena dendrobii Fan et Guo) and Trichoderma and an application method thereof , has the effects of promoting the rapid growth of dendrobium seedlings, increasing the content of polysaccharides and dendrobine. (2) Background technology [0002] In the cultivation process of the rare medicinal plant Dendrobium, the industrialization work is very difficult due to the unique complexity of its growth and development. One is that Dendrobium has a lot of flowers, and only a very small number of them can bear fruit. The fruit is a capsule, the seed is as fine as dust, the development of the embryo does not exceed the spherical stage, and it lacks endosperm tissue. The germination rate under natural conditions is less than 5%, and the growth and development of plants germinated from seeds ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/02A01P21/00A01H4/00A01G1/04C05G5/00
Inventor 王慧中徐祥彬应奇才
Owner HANGZHOU NORMAL UNIVERSITY
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