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Detection method for NR-SOB (nitrate reduction-sulfion oxidation bacteria) in oilfield water sample

An NR-SOB, oilfield water sample technology, applied in the field of microbial detection, can solve the problem of NR-SOB bacteria detection, but can not solve the problems of optimizing the hydrogen sulfide treatment agent and oxygen content requirements in oil reservoirs, and achieves convenient detection, simple use and operation, The effect of simple production process

Inactive Publication Date: 2013-02-13
E TECH ENERGY TECH DEV CORP
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AI Technical Summary

Problems solved by technology

However, there are strict requirements on oxygen content in the culture conditions, and technical problems such as optimization of hydrogen sulfide treatment agents in oil reservoirs cannot be solved without solving the detection problem of NR-SOB bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The detection method of nitrate reducing-sulfur ion oxidizing bacteria NR-SOB in oilfield water samples comprises the following steps:

[0039] 1. Production of NR-SOB bacteria detection bottle:

[0040] (1) Material cleaning

[0041] Wash the 10mL glass bottle twice in washing powder water, wash the rubber stopper and aluminum foil cover once in washing powder water, then rinse with clean water until there is no foam, then clean the glass bottle, rubber stopper and aluminum foil cover with distilled water, and air dry Residual water, set aside.

[0042] (2) Culture medium preparation

[0043] Under normal temperature and pressure, prepare the medium according to the NR-SOB test medium preparation instructions in the table below:

[0044] NR-SOB medium (g / L): NaHCO 3 1.9, sodium acetate 0.68, KNO 3 1, NH 4 Cl 0.02, (NH 4 ) 2 SO 4 0.13, KH 2 PO 4 0.6, Na 2 HPO 4 0.7, NaCl 7, CaCl 2 2H 2 O 0.27, MgSO 4 ·7H 2 O 0.78, Na 2 S·9H 2 O 0.6 (add after ster...

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Abstract

The invention belongs to the technical field of microbiological detection, and relates to a detection method for NR-SOB (nitrate reduction-sulfion oxidation bacteria) in an oilfield water sample, which comprises the following steps: firstly, material cleaning: cleaning a glass ware, rubber plugs and aluminium foil covers; secondly, preparing a NR-SOB testing culture medium; thirdly, sterilization: accommodating the prepared NR-SOB testing culture medium into the glass ware, sealing by a gauze, and using a steam sterilization pot to sterilize; fourthly, deoxidization, sulfur treatment and split charging: deoxidization: placing the culture medium after sterilization into an anaerobic workstation for deoxidization, sulfur treatment: adding a Na2S.9H2O mother liquor into the culture medium, and split charging: using a liquid-moving machine to accommodate the culture medium into cleaned glass bottles respectively; and fifthly, NR-SOB detection: adopting an extinct dilution method to detect the number of bacteria in a water sample to be detected. The detection method has the advantages of simplicity in operation, convenience for preparation, convenience and quickness for testing, accurate data, high reliability, economy and practicability, quickness, accuracy, convenience for microbial detection in an oilfield and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial detection, in particular to a detection method for nitrate reduction-sulfur ion oxidation bacteria NR-SOB in oil field water samples. Background technique [0002] At present, in the process of microbial treatment of hydrogen sulfide in oil reservoirs, nitrate-reducing bacteria are generally concerned, and the principle of biological competition inhibition is used to eliminate hydrogen sulfide of oil reservoir biogenesis. With the development of science and technology, it is found that some oil reservoirs contain other microorganisms related to sulfur metabolism, such as NR-SOB bacteria. NR-SOB belongs to anaerobic microorganisms. It can eliminate biogenic hydrogen sulfide, and can also eliminate non-biogenic hydrogen sulfide. However, its cultivation conditions have relatively strict requirements on oxygen content, and technical problems such as optimization of hydrogen sulfide treatment agent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/12C12Q1/06
Inventor 胡欣
Owner E TECH ENERGY TECH DEV CORP
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