Kit for detecting mRNA (messenger ribonucleic acid) expression quantity of U BCR fusion gene

Abstract

The invention discloses a kit for detecting the mRNA (messenger ribonucleic acid) expression quantity of U BCR fusion gene, belonging to the field of biotechnology. The kit comprises a detection primer, a fluorescent probe, a cDNA (complementary deoxyribonucleic acid) first strand synthesis reagent, fluorescent quantitative PCR (polymerase chain reaction) mixed solution, a negative control and a positive control, wherein the detection primer and the fluorescent probe comprise a U BCR fusion gene primer, an internal reference gene ABL primer and a Taqman fluorescent probe. The U BCR fusion gene is mainly found in chronic neutrophilic leukaemia with Ph+, and the gene code is high in the activity of tyrosine protein kinase, so that cells perform excessive multiplication and differentiation in case of no independence on cell factors, and normal cell apoptosis is inhibited. The mRNA level of the U BCR fusion gene is detected by adopting fluorescent quantitative PCR, and the specificity and the sensitivity of the detection result are remarkably improved. Via the kit, a novel rapid, simple and convenient gene diagnosis technology is provided for diagnosis, prediction and prognosis, and chemotherapy for chronic neutrophilic leukaemia.

Description

technical field [0001] The invention relates to the fluorescent quantitative PCR technology in the field of biotechnology, in particular to a kit for detecting the expression level of U BCR fusion gene mRNA. Background technique [0002] Chronic Myelocytic Leukemia (CML) is a malignant tumor caused by abnormal proliferation of pluripotent stem cells. The real understanding of the disease comes from the discovery of the Philadelphia Chromosome (Ph). Data show that more than 90% of CML Ph is present in all cases, and this chromosome is formed by the mutual translocation of chromosome 9 and chromosome 22, that is, t(9,22) (q34:q11). The translocation makes the end of the long arm of chromosome 9 (9q34) The c-abl proto-oncogene breaks at the 5' end of the second exon, and then fuses with the 3' end of the c-BCR fusion gene at the end of the long arm of chromosome 22 (22q11) to form a bcr / abl fusion gene. Among them, the breakpoint in the BCR fusion gene of some CML patients is ...

AI Technical Summary

Problems solved by technology

[0005] Real-time fluorescence quantitative PCR technology has realized the leap from qualitative to real quantitative PCR, and provides an effective detection tool for the quantitative detection of human disease genes. Compared with ordinary PCR, it has enhanced specificity, improved sensitivity and rapid detection. And reduce pollution and other characteristics, but there is no relevant report on the detection of U BCR gene in chronic myeloid leukemia by fluorescent quantitative PCR method

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