Detection method for measuring residual quantity of urea in bean sprout by high performance liquid chromatography

A high-performance liquid chromatography and residue technology, which is applied in the field of high-performance liquid chromatography for the determination of urea residues in bean sprouts, can solve the problems of false positives and large interference of colorimetric results, achieve high accuracy, reduce matrix Interference, the effect of low detection limit

Inactive Publication Date: 2013-02-13
谱尼测试集团股份有限公司
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Problems solved by technology

This method is applied to the determination of urea in bean sprouts. Because the bean sprouts matrix is ​​light yellow, the contrast color results are greatly disturbed, which may easily cause false positives.

Method used

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  • Detection method for measuring residual quantity of urea in bean sprout by high performance liquid chromatography

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The mensuration of urea residue in certain commercially available bulk soybean sprouts of embodiment 1

[0021] 1. Analysis steps

[0022] Weigh 2.00g (accurate to 0.001g) of the crushed bean sprouts sample in a 50mL centrifuge tube, add 10mL of absolute ethanol to shake and extract for 30min, centrifuge at 8000r / min for 5min, and filter the supernatant through a 0.45μm organic filter membrane. Measurement. Pipette 0.5 mL of the filtrate into a 1.5 mL injection bottle, add 100 μL of 0.02 mol / L 9-hydroxyxanthene and 100 μL of 1.5 mol / L hydrochloric acid solution, and derivatize in the dark for 20 minutes before testing.

[0023] 2. High performance liquid chromatography conditions

[0024] Instrument: Agilent1200 liquid chromatograph equipped with a fluorescence detector; Chromatographic column: a chromatographic column (4.6×150mm, 5μm) with carbon octadecyl bonded silica gel as a filler; mobile phase: acetonitrile + 0.02mol / L sodium acetate solution (gradient elution...

Embodiment 2

[0031] The mensuration of residual amount of urea in multiple commercially available samples of embodiment 2

[0032] Seven kinds of commercially available bean sprouts were randomly purchased, and the experiment was carried out according to the above-mentioned pretreatment method and instrumental analysis method. The samples were measured in parallel three times, and the results are shown in Table 2.

[0033] Table 2 Commercially available bean sprouts content assay results (n=3)

[0034] (unit: mg / kg)

[0035]

[0036]From the above results, it can be seen that the bean sprouts samples of various brands have detected different levels of urea residues (organic bean sprouts are between 5.5-11.0 mg / kg, and the content of bulk bean sprouts is slightly higher between 21.4-37.4). The reason for the analysis may be During the production process of bean sprouts, amino acid metabolism may produce urea, which will have a certain impact on the test results. Therefore, it is recomm...

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Abstract

The invention discloses a detection method for measuring residual quantity of urea in bean sprout by high performance liquid chromatography, which comprises the following steps of: extracting the urea in the bean sprout by oscillating absolute ethyl alcohol; precipitating protein; centrifuging an extracting solution; deriving on an acid condition by using 9-hydroxyxanthene serving as a derivating agent; separating a derivation product by a chromatographic column which takes octadecyl silane as a filling agent; detecting by using a fluorescence detector; and quantifying by an external standard method. The method has the characteristics of qualitative accuracy, avoidance of false positive, fast quantification and the like, and is suitable for detection on the residual quantity of the urea in the bean sprout.

Description

technical field [0001] The invention relates to a method for detecting residual urea in bean sprouts, in particular to a method for detecting residual urea in bean sprouts by high performance liquid chromatography. Background technique [0002] Urea, also known as carbonamide, carbonamide, and urea, is an organic compound composed of carbon, nitrogen, oxygen, and hydrogen, also known as urea, and its chemical formula is CO(NH 2 ) 2 , white crystal or powder. It is a product of animal protein metabolism and is usually used as nitrogen fertilizer for plants. Urea is synthesized in the liver and is a nitrogenous metabolite excreted by mammals. Urea is the first organic compound obtained by artificially synthesizing inorganic substances, and has been widely used in agricultural production, cosmetics and other fields. [0003] In recent years, unscrupulous producers and operators have illegally added urea during the production of bean sprouts to promote their rapid growth. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 宋薇
Owner 谱尼测试集团股份有限公司
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