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Method for preparing selenylation poly mannuronic acid and application of selenylation poly mannuronic acid

A technology of polymannuronic acid and mannuronic acid, which is applied in the field of preparation of selenized polymannuronic acid, can solve the problems that have not been reported on the effect of selenium polysaccharides on AD, and achieve the prevention and treatment of Alzheimer's disease , high yield and low cost effect

Active Publication Date: 2013-02-20
SHENZHEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the effect of polysaccharides on Alzheimer's disease (AD), but there is no report on the effect of selenium polysaccharides on AD

Method used

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  • Method for preparing selenylation poly mannuronic acid and application of selenylation poly mannuronic acid
  • Method for preparing selenylation poly mannuronic acid and application of selenylation poly mannuronic acid
  • Method for preparing selenylation poly mannuronic acid and application of selenylation poly mannuronic acid

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preparation example Construction

[0017] The embodiment of the present invention provides a kind of preparation method of selenized polymannuronic acid, it comprises the following steps:

[0018] S01: Preparation of sulfonated polymannuronic acid: suspend polymannuronic acid (PM) in anhydrous organic solvent, add sulfur trioxide-pyridine complex dissolved in anhydrous organic solvent under stirring condition, React at a constant temperature of 100°C for 1 to 10 hours, add 2 to 4 times the volume of 95% ethanol, centrifuge the reaction product, then wash with 95% ethanol, add water to dissolve, adjust the pH to neutral with alkali, dialyze, and filter , freeze-dried to obtain sulfonated polymannuronic acid (S-PM);

[0019] S02: Preparation of selenized polymannuronic acid: the sulfonated polymannuronic acid and selenite are mixed in acidic solution, Ba 2+ Under the catalysis of 20 ~ 100 ℃ constant temperature for 2 ~ 20 hours, the molar ratio of sulfonated polymannuronic acid to barium chloride is 1:1 ~ 100, a...

Embodiment 1

[0029] Suspend 100 mg of dried polymannuronic acid (PM) in DFM, add 600 mg of sulfur trioxide-pyridine complex dissolved in 5 mL of anhydrous DMF under stirring conditions, react at a constant temperature of 60 ° C for 4 hours, add 3 Double the volume of 95% ethanol, centrifuge the reaction product, wash with 95% ethanol, add water to dissolve, adjust the pH to neutral with alkali, dialyze, filter, and freeze-dry to obtain sulfonated polymannuronic acid, wherein the sulfur content is 12.5%, DS=1.29, and the average yield is 78%;

[0030] Preparation of selenized mannuronic acid: the 100mg sulfonated polymannuronic acid and 200mg selenite were added to 50mL0.5% HNO 3 In solution, 25mg BaCl 2 Under the catalysis of 60 ℃ reaction 8h, adding sulfate to remove Ba 2+ , centrifuge to take the supernatant, adjust the pH to neutral with alkali, and then dialyze with a dialysis bag with a molecular weight cut-off of 1000, until the ascorbic acid detection dialysis bag liquid does not ...

Embodiment 2

[0032] Suspend 100 mg of dried polymannuronic acid (PM) in DFM, add 600 mg of sulfur trioxide-pyridine complex dissolved in 5 mL of anhydrous DMF under stirring conditions, react at a constant temperature of 20 ° C for 10 hours, add 2 Double the volume of 95% ethanol, centrifuge the reaction product, wash with 95% ethanol, add water to dissolve, adjust the pH to neutral with alkali, dialyze, filter, and freeze-dry to obtain sulfonated polymannuronic acid;

[0033] Preparation of selenized mannuronic acid: add the 100mg sulfonated polymannuronic acid and 10mg selenite to 50mL0.5% HNO 3 In solution, 25mg Ba(NO 3 ) 2 Under the catalysis of 20 ℃ reaction 20h, adding sulfate to remove Ba 2+ , centrifuge to take the supernatant, adjust the pH to neutral with alkali, and then dialyze with a dialysis bag with a molecular weight cut-off of 1000 until the ascorbic acid detection dialysis bag liquid does not turn red. Filtrate and freeze-dry to obtain the selenized polymannuronic acid...

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Abstract

The invention relates to the technical field of selenylation polysaccharide and provides a method for preparing selenylation poly mannuronic acid. The method comprises steps of suspending poly mannuronic acid in an anhydrous organic solvent; adding a sulfur trioxide-pyridine compound dissolved by the anhydrous organic solvent with stirring; conducting reaction for 1-10 hours at the constant temperature from 20 DEG C to 100 DEG C; adding ethanol; centrifugalizing a reaction product; cleaning by using the ethanol; adding water and dissolving; adjusting the Potential of Hydrogen (PH) value to the neuter by using alkali; conducting dialysis; conducting filtering; conducting freezing and drying; obtaining sulfonated poly mannuronic acid; subjecting the sulfonated poly mannuronic acid and selenite to the reaction in the acid solution at the catalysis of Ba2+; adding sulphate; extracting supernatant fluid through centrifugation; adjusting the Potential of Hydrogen (PH) value to the neuter by using the alkali; conducting dialysis; conducting filtering; conducting freezing and drying; and obtaining the selenylation poly mannuronic acid. The invention also provides application of the selenylation poly mannuronic acid which is prepared through the method in medicines and health products for prevention and curing of Alzheimer's disease.

Description

technical field [0001] The invention belongs to the technical field of selenized polysaccharides, and in particular relates to a preparation method of selenized polymannuronic acid and its application. Background technique [0002] Alginate is a water-soluble acidic polysaccharide, which is mainly extracted from the cell walls of brown algae such as macroalgae, sargasso, and kelp, and has a unique structure and biological activity. Its structure consists of α-1,4-L-guluronic acid (α-1,4-L-guluronic acid, abbreviated as G) and β-l,4-D-mannuronic acid (β-l , 4-D-mannuronicacid, abbreviated as M) is a linear anionic polysaccharide linked by 1-4 glycosidic bonds. The whole molecule is composed of three fragments: polymannuronic acid segment (Poly-mannuronate, PM), polyguluronic acid segment (Poly-guluronate, PG) and polymannuronic acid-guluronic acid hybrid segment (PMG block). Therefore, a single polymannuronic acid can be isolated by the existing acid hydrolysis method. Th...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P25/28A23L1/09
Inventor 朱志杰刘琼续旭倪嘉缵
Owner SHENZHEN UNIV
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