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Synthesis method of polypeptide-nanogold particle drug carrier

A synthesis method and nano-gold technology, applied in the field of biomedical materials and nanometers, can solve the problems of limited application and poor biocompatibility of small organic molecules, and achieve the effects of controlled release, low toxicity and easy promotion.

Inactive Publication Date: 2013-03-13
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the poor biocompatibility of organic small molecules limits their application in biological

Method used

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  • Synthesis method of polypeptide-nanogold particle drug carrier
  • Synthesis method of polypeptide-nanogold particle drug carrier
  • Synthesis method of polypeptide-nanogold particle drug carrier

Examples

Experimental program
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Embodiment 1

[0029] provides a polypeptide — Synthesis method of nano gold particle drug carrier. First, gold nanoparticles with a particle size of 20 nm were prepared by reducing chloroauric acid with sodium citrate; the peptide sequence used was DDNNLAC, namely Asp-Asp-Asp-Asp-Asn-Leu-Ala-Cys (SEQ ID NO1) and DDDD(PPG ) 2 LVPRGS (PPG) 3 G is

[0030] Asp-Asp-Asp-Asp-Pro-Pro-Gly-Pro-Pro-Gly-Leu-Val-Pro-Arg-Gly-Ser-Pro-Pro-Gly-Pro-Pro-Gly-Pro-Pro-Gly- Gly-Cys (SEQ ID NO2) mixture, the molar ratio of the two polypeptide molecules is 1:1 to obtain a polypeptide mixture; this polypeptide mixture is added to the gold nano solution with a particle size of 20 nm, so that the molar concentration of the gold nano solution 10 μM, the total molar concentration of the peptide is 1 mM (the molar ratio of nano-gold and peptide is 1:100), shake for 10 min, centrifuge at 12000 rpm, discard the supernatant, and add the same amount of PBS buffer as the supernatant (pH7.4), and finally synthesized the ...

Embodiment 2

[0033] The encapsulated drug molecule is 2 mM paclitaxel, and the total molar concentration of the polypeptide is 3 mM, in which the peptide — The molar ratio of gold nanoparticle complexes to drug molecules is 1:300). Other steps are with embodiment 1.

Embodiment 3

[0035]The total molar concentration of the polypeptide was 3 mM, the concentration of BODIPY was 3 mM, and the reaction was allowed to stand at room temperature for 1 h. Other steps are with embodiment 1.

[0036] 1 mL, 200 nM peptide — The gold nanoparticle drug carrier was mixed with the culture medium and then added with 1×10 5 cells in a culture dish at 5% CO 2 , reacted at 37°C for 2 h. Wash 3 times with PBS, remove the supernatant, and finally add PBS buffer. Cell fluorescence was observed by confocal microscope, and the fluorescence intensity of cells induced by enzyme cleavage was significantly higher than that of control cells. The results are shown in the appendix image 3 .

[0037]

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Abstract

Belonging to the technical fields of biomedical materials and nanotechnologies, the invention discloses a synthesis method of a polypeptide-nanogold particle drug carrier. A drug delivery system comprises polypeptide modified nanogold particles and a drug molecular model encapsulated therein, or in other words, the nanogold particles are modified by polypeptide molecules on them so as to prepare the nanogold particle drug delivery system. By adjusting the proportions of stable polypeptides and functional polypeptides in an initial reaction mixture, polypeptide-nanogold carriers with different packaging abilities can be obtained. Experiments prove that, the method has the advantages of simple operation and less time consumption, and has a very good cell delivery effect on the drug molecular model. The drug carrier synthesized by the method has good biocompatibility and small toxic and side effect in organisms, thus being applicable to cancer treatment.

Description

technical field [0001] The invention relates to the fields of biomedical materials and nanotechnology, in particular to a method for synthesizing a polypeptide-nano gold particle drug carrier. [0002] Background technique [0003] At present, the most effective methods for treating malignant tumors are still radiotherapy and chemotherapy. However, most drugs can destroy normal tissue cells while effectively killing tumor cells. In order to improve drug targeting and reduce drug toxicity, nano-drug delivery carriers have attracted widespread attention. [0004] Due to the advantages of small size, good biocompatibility and low toxicity, nano drug delivery system has been widely used in the field of drug delivery. Among them, gold nanoparticles have the advantages of good biocompatibility, small particle size and easy surface modification, and are a kind of nano drug carrier with great potential. At present, many groups at home and abroad are conducting research in this a...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61K47/02A61K45/00A61P35/00
Inventor 王建浩邱琳王车礼蒋鹏举夏江
Owner CHANGZHOU UNIV
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