Method for preparing phosphatidylserine and phosphatidylcholine
A technology for phosphatidylserine and phosphatidylcholine, which is applied in the field of preparing phosphatidylserine and phosphatidylcholine, can solve the problems of limiting the wide application of phosphatidylserine, high product price and high production cost, and achieves improved utilization value and shortened time. The effect of high production time and productivity
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Embodiment 1
[0030] Add soybean lecithin powder into a three-neck flask containing 80% ethanol with 5 times the mass of soybean powder lecithin, stir, heat in a water bath to 60°C for 120 minutes, filter, and take the filtrate in a concentration tank or a rotary evaporator under a vacuum of -0.085MPa Distill at 65°C for 2 hours, recover ethanol to obtain crude phosphatidylcholine, and dry the filter residue in an oven at 65°C for 5 hours to obtain crude phosphatidylserine.
[0031] Select 200-300 mesh silica gel, first activate for 2 hours, and activate at a temperature of 105°C; secondly, use a wet method to load the column, and the mass ratio of crude phosphatidylserine to silica gel is 1:100, and the crude phosphatidylserine is dissolved in chloroform. The concentration of the solution is 0.1g / mL; elution is carried out again in the column, and the eluent is mixed and dissolved with chloroform / methanol, wherein the volume ratio of chloroform / methanol is 75 / 25, the elution flow rate is 3m...
Embodiment 2
[0035] Add soybean lecithin powder into a three-necked flask containing 4 times the mass of soybean powder lecithin and 90% ethanol, stir, heat in a water bath to 65°C for 100 minutes, filter, take the filtrate and distill it in a rotary evaporator at a vacuum of -0.085MPa at 70°C After 3 hours, ethanol was recovered to obtain crude phosphatidylcholine, and the filter residue was dried in an oven at 70° C. for 7 hours to obtain crude phosphatidylserine.
[0036] Select 200-300 mesh silica gel, first activate for 1.5h, and activate at a temperature of 110°C; secondly, use a wet method to load the column, and the mass ratio of crude phosphatidylserine to silica gel is 1:120, and the crude phosphatidylserine is dissolved in chloroform. The concentration of the final solution is 0.08g / mL; elution is carried out again in the column, and the eluent is mixed and dissolved with chloroform / methanol, wherein the volume ratio of chloroform / methanol is 75 / 25, the elution flow rate is 2mL / m...
Embodiment 3
[0039] Add soybean lecithin powder into a three-neck flask containing 3.5 times the mass of soybean powder lecithin and 75% ethanol, heat it in a water bath to 55°C for 90 minutes, filter, and take the filtrate to distill for 2.5 hours at 60°C in a rotary evaporator with a vacuum degree of -0.085MPa , recovering ethanol to obtain crude phosphatidylcholine, and drying the filter residue in an oven at 60°C for 6 hours to obtain crude phosphatidylserine.
[0040] Select 200-300 mesh silica gel, first activate for 1.5h, and activate the temperature at 100°C; secondly, apply the wet method to the column, the mass ratio of crude phosphatidylserine to silica gel is 1:90, the crude phosphatidylserine is dissolved in chloroform, dissolved The concentration of the final solution is 0.1g / mL; elution is carried out again in the column, and the eluent is mixed and dissolved with chloroform / methanol, wherein the volume ratio of chloroform / methanol is 70 / 30, the elution flow rate is 3mL / s, an...
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