Method for preparing phosphatidylserine and phosphatidylcholine

A technology for phosphatidylserine and phosphatidylcholine, which is applied in the field of preparing phosphatidylserine and phosphatidylcholine, can solve the problems of limiting the wide application of phosphatidylserine, high product price and high production cost, and achieves improved utilization value and shortened time. The effect of high production time and productivity

Active Publication Date: 2013-03-13
成都圆大生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

People began to try to extract phosphatidylserine or phosphatidylcholine from natural plants, but they also faced the problems of complicated steps in the extraction method, low yield and expensive products
In recent years, the method for synthesizing phosphatidylserine by conversion of phospholipase D at home and abroad has attracted attention. Because phospholipase D has a high cost as a high-tech product, and the catalytic ability of phospholipase D on the market is low, it is difficult to convert and produce phosphatidylserine Phospholipase D requires a large amount and the reaction time is as long as 10-20 hours, resulting in high production costs and time-consuming, which limits the further extensive application of phosphatidylserine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Add soybean lecithin powder into a three-neck flask containing 80% ethanol with 5 times the mass of soybean powder lecithin, stir, heat in a water bath to 60°C for 120 minutes, filter, and take the filtrate in a concentration tank or a rotary evaporator under a vacuum of -0.085MPa Distill at 65°C for 2 hours, recover ethanol to obtain crude phosphatidylcholine, and dry the filter residue in an oven at 65°C for 5 hours to obtain crude phosphatidylserine.

[0031] Select 200-300 mesh silica gel, first activate for 2 hours, and activate at a temperature of 105°C; secondly, use a wet method to load the column, and the mass ratio of crude phosphatidylserine to silica gel is 1:100, and the crude phosphatidylserine is dissolved in chloroform. The concentration of the solution is 0.1g / mL; elution is carried out again in the column, and the eluent is mixed and dissolved with chloroform / methanol, wherein the volume ratio of chloroform / methanol is 75 / 25, the elution flow rate is 3m...

Embodiment 2

[0035] Add soybean lecithin powder into a three-necked flask containing 4 times the mass of soybean powder lecithin and 90% ethanol, stir, heat in a water bath to 65°C for 100 minutes, filter, take the filtrate and distill it in a rotary evaporator at a vacuum of -0.085MPa at 70°C After 3 hours, ethanol was recovered to obtain crude phosphatidylcholine, and the filter residue was dried in an oven at 70° C. for 7 hours to obtain crude phosphatidylserine.

[0036] Select 200-300 mesh silica gel, first activate for 1.5h, and activate at a temperature of 110°C; secondly, use a wet method to load the column, and the mass ratio of crude phosphatidylserine to silica gel is 1:120, and the crude phosphatidylserine is dissolved in chloroform. The concentration of the final solution is 0.08g / mL; elution is carried out again in the column, and the eluent is mixed and dissolved with chloroform / methanol, wherein the volume ratio of chloroform / methanol is 75 / 25, the elution flow rate is 2mL / m...

Embodiment 3

[0039] Add soybean lecithin powder into a three-neck flask containing 3.5 times the mass of soybean powder lecithin and 75% ethanol, heat it in a water bath to 55°C for 90 minutes, filter, and take the filtrate to distill for 2.5 hours at 60°C in a rotary evaporator with a vacuum degree of -0.085MPa , recovering ethanol to obtain crude phosphatidylcholine, and drying the filter residue in an oven at 60°C for 6 hours to obtain crude phosphatidylserine.

[0040] Select 200-300 mesh silica gel, first activate for 1.5h, and activate the temperature at 100°C; secondly, apply the wet method to the column, the mass ratio of crude phosphatidylserine to silica gel is 1:90, the crude phosphatidylserine is dissolved in chloroform, dissolved The concentration of the final solution is 0.1g / mL; elution is carried out again in the column, and the eluent is mixed and dissolved with chloroform / methanol, wherein the volume ratio of chloroform / methanol is 70 / 30, the elution flow rate is 3mL / s, an...

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Abstract

The invention relates to a method for preparing phosphatidylserine and phosphatidylcholine, and belongs to the field of food health-care products. The method comprises the following steps of: extracting soybean meal phospholipid which is used as a raw material by an organic solvent, and then distilling and drying to respectively obtain a phosphatidylcholine crude product and a phosphatidylserine crude product; and separating and purifying the phosphatidylcholine crude product and the phosphatidylserine crude product through a silica gel column of 200-300 meshes, eluting with a mixed organic solvent, concentrating the eluant, and drying to obtain phosphatidylserine and phosphatidylcholine. According to the method, the purities of the phosphatidylserine and the phosphatidylcholine meet requirements of new resource foods, so that the phosphatidylserine and the phosphatidylcholine can be used as new resource dietary supplements and have high economic added values; and the phosphatidylserine and the phosphatidylcholine are prepared at the same time by effectively combining simple extraction of the phosphatidylserine and the simple extraction of the phosphatidylcholine. By the method, the production time is shortened, the production cost is reduced, the production rate is improved, the operation is simple, and the products have safety.

Description

technical field [0001] The invention belongs to the field of food health products, in particular to a method for preparing phosphatidylserine and phosphatidylcholine. [0002] Background technique [0003] Phosphatidylserine (Phosphatidylserine, PS) is a naturally occurring phospholipid family. It is an important component of cell membrane phospholipids and the only phospholipid that can regulate the functional status of key proteins in cell membranes. Phosphatidylserine widely exists in the seeds of animals, higher plants, and biofilms of microorganisms, and plays an important role in regulating many cellular metabolic processes. Animal experiments and clinical applications have shown that phosphatidylserine has a significant effect on preventing and treating Alzheimer's disease, improving memory and cognitive ability, relieving mental stress and depression. There are currently no reports of side effects of PS, and a large double-blind study by Dr. Cenacchi in Italy showe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10
Inventor 李鹏婧郭春林张勇李沅锴苟荣吴向东
Owner 成都圆大生物科技有限公司
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