Method for detecting cocaine by using quartz crystal microbalance

A quartz crystal and microbalance technology, applied in the field of biosensors, can solve problems such as easy addiction, high toxicity, physical and psychological damage, and achieve the effects of saving manpower, realizing high automation, and reducing costs

Inactive Publication Date: 2014-09-03
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of its high toxicity and easy addiction, it has been replaced by other local anesthetics recently. Long-term smoking of cocaine will cause physical and psychological dependence, causing great physical and psychological harm

Method used

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  • Method for detecting cocaine by using quartz crystal microbalance
  • Method for detecting cocaine by using quartz crystal microbalance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step 1. Soak the cleaned gold-based QCM chip in 100 μL DNA assembly solution and incubate for 2 hours, then take out the QCM chip, rinse it with ethanol, and dry it with nitrogen to obtain a QCM chip with surface self-assembled DNA; the DNA assembly The solution is prepared by mixing DNA, NaCl and PBS buffer solution with a pH value of 7.2 at a concentration of 0.1M. The concentration of NaCl in the DNA assembly solution is 0.2M, and the concentration of DNA is 0.2 μM; the DNA is sulfhydryl-modified DNA, The DNA sequence (SEQ ID NO.1) is: 5'-TCA CAG ATG AGT AAA AAA AAA AAA-3';

[0025] Step 2. At room temperature, incubate the QCM chip of surface self-assembled DNA described in step 1 with an ethanol solution of 2 μM mercaptopolyethylene glycol for 2 h to seal the redundant sites on the chip surface, then take out the QCM chip and use Rinse with ethanol and blow dry with nitrogen;

[0026] Step 3. Dissolve 0.1mol (11.5g) of NHS in 250mL of dichloromethane, magnetically...

Embodiment 2

[0031] Step 1. Soak the cleaned gold-based QCM chip in 100 μL DNA assembly solution and incubate for 2 hours, then take out the QCM chip, rinse it with ethanol, and dry it with nitrogen to obtain a QCM chip with surface self-assembled DNA; the DNA assembly The solution is prepared by mixing DNA, NaCl and PBS buffer solution with a pH value of 7.2 at a concentration of 0.1M. The concentration of NaCl in the DNA assembly solution is 0.2M, and the concentration of DNA is 0.2 μM; the DNA is sulfhydryl-modified DNA, The DNA sequence (SEQ ID NO.1) is: 5'-TCA CAG ATG AGT AAA AAA AAA AAA-3';

[0032] Step 2. At room temperature, incubate the QCM chip of surface self-assembled DNA described in step 1 with an ethanol solution of 2 μM mercaptopolyethylene glycol for 2 h to seal the redundant sites on the chip surface, then take out the QCM chip and use Rinse with ethanol and blow dry with nitrogen;

[0033] Step 3. Dissolve 0.1mol (11.5g) of NHS in 250mL of dichloromethane, magnetically...

Embodiment 3

[0038] Step 1. Soak the cleaned gold-based QCM chip in 100 μL DNA assembly solution and incubate for 2 hours, then take out the QCM chip, rinse it with ethanol, and dry it with nitrogen to obtain a QCM chip with surface self-assembled DNA; the DNA assembly The solution is prepared by mixing DNA, NaCl and PBS buffer solution with a pH value of 7.2 at a concentration of 0.1M. The concentration of NaCl in the DNA assembly solution is 0.2M, and the concentration of DNA is 0.2 μM; the DNA is sulfhydryl-modified DNA, The DNA sequence (SEQ ID NO.1) is: 5'-TCA CAG ATG AGT AAA AAA AAA AAA-3';

[0039] Step 2. At room temperature, incubate the QCM chip of surface self-assembled DNA described in step 1 with an ethanol solution of 2 μM mercaptopolyethylene glycol for 2 h to seal the redundant sites on the chip surface, then take out the QCM chip and use Rinse with ethanol and blow dry with nitrogen;

[0040] Step 3. Dissolve 0.1mol (11.5g) of NHS in 250mL of dichloromethane, magnetically...

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Abstract

The invention discloses a method for detecting cocaine by using a quartz crystal microbalance. The method comprises the steps of: I, preparing a QCM (Quartz Crystal Monitor) chip with self-assembled DNA on the surface of the QCM chip; II, closing excessive sites on the surface of the chip; III, preparing an initiator-labeled DNA (Deoxyribonucleic Acid) solution; IV, jointly hatching the closed chip by using an aptamer DNA solution and the initiator-labeled DNA solution, placing the closed chip into a reactor of the quartz crystal microbalance, introducing a buffer solution, and reading a frequency F1; V, taking out the chip for a surface initiated polymerization reaction; and VI, placing the chip subjected to the surface initiated polymerization reaction into the reactor, introducing the buffer solution, reading a frequency F2, then introducing a detecting object, reading a frequency3, calculating differences delta F' and delta F'' between F2 and F1 as well as between F2 and F3, and determining that the detecting object contains the cocaine when the ratio of delta F'' to delta F' is not less than 30%. As the quartz crystal microbalance is used as a detection tool, the method for detecting the cocaine by using the quartz crystal microbalance has the advantages of real-time detection and the like.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to a method for detecting cocaine by using a quartz crystal microbalance. Background technique [0002] Cocaine, also known as cocaine, is an alkaloid isolated from coca leaves. Cocaine is a powerful local anesthetic with high fat solubility and good affinity to nerve tissue, and it is a central nervous system stimulant. Because of its high toxicity and easy addiction, it has been replaced by other local anesthetics recently. Long-term smoking of cocaine will cause physical and psychological dependence, which will cause great physical and psychological harm. The rapid detection of cocaine is of great significance. Traditional detection methods include high performance liquid chromatography, gas chromatography-mass spectrometry, thin layer chromatography and high performance capillary electrophoresis. The aptamer-based detection method has high resolution and high affinity, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N5/02
Inventor 马宏伟朱志强汪杰
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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