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Analysis system with combined capillary tube ion chromatography and mass spectrum

An analysis system, ion chromatography technology, applied in the direction of analysis materials, material separation, measurement devices, etc., can solve the problems of poor identification and separation, ICMS peak broadening and insufficient sensitivity, so as to improve separation selectivity and sensitivity, unique selectivity and detection sensitivity, high-efficiency separation effect

Inactive Publication Date: 2013-03-13
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the chromatographic column used in the present invention is that the current LCMS technology and ion chromatography technology have poor identification and separation effects on water-soluble components such as organic acids, sugars, alcohols, and the problems of ICMS peak broadening and insufficient sensitivity

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  • Analysis system with combined capillary tube ion chromatography and mass spectrum
  • Analysis system with combined capillary tube ion chromatography and mass spectrum
  • Analysis system with combined capillary tube ion chromatography and mass spectrum

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Instrument selection: ICS-5000 multifunctional chromatographic device (thermo fisher scientific company in the United States). Including dual pump (DP) module, detector / chromatography (DC) module, auto sampler (AS) module, eluent automatic generator (EG) module, capillary chromatography (IC-cube) module, dual pump module Contains an analytical pump, a capillary pump, and a conductivity detector. The IC-cube module includes a switching valve (0.4uL), a column oven, a cation membrane electrolytic ion exchange device (ACES300), a degassing box (EG degasser), a carbonate removal device (CRD), a capillary ion chromatography column (IonSwift Max-100Capillary column 0.4mm×250mm) and guard column (0.4mm×50mm). LTQ-XL ion hydrazine mass spectrometer (thermo fisher scientific company, USA).

[0049] system connection as Figure 5 Shown: Pump 1 of the capillary ion chromatograph is connected to the KOH eluent bottle, the eluent bottle is connected to the degassing box, the dega...

Embodiment 2

[0057] Instrument selection: ICS-5000 multifunctional chromatographic device (thermo fisher scientific company in the United States). Including dual pump (DP) module, detector / chromatography (DC) module, autosampler (AS) module, eluent automatic generator (EG) module, capillary chromatography (IC-cube) module, dual pump module Contains an analytical pump, a capillary pump, and a conductivity detector. The IC-cube module includes a switching valve (0.4uL), a column oven, a cation membrane electrolytic ion exchange device (ACES300), a degassing box (EG degasser), a carbonate removal device (CRD), a capillary ion chromatography column (CarboPacPA20Capillarycolumn 0.4 mm×250mm) and guard column (0.4mm×50mm). LTQ-XL ion hydrazine mass spectrometer (U.S. thermo fisherscientific company).

[0058] system connection as Figure 8 Shown: Pump 1 of the capillary ion chromatograph is connected to the KOH eluent bottle, the eluent bottle is connected to the degassing box, the degassing ...

Embodiment 3

[0063] Instrument selection: ICS-5000 multifunctional chromatographic device (thermo fisher scientific company in the United States). Including dual pump (DP) module, detector / chromatography (DC) module, auto sampler (AS) module, eluent automatic generator (EG) module, capillary chromatography (IC-cube) module, dual pump module Contains an analytical pump, a capillary pump, and a conductivity detector. The IC-cube module includes a switching valve (0.4uL), a column oven, an anion membrane electrolytic ion exchange device (CCES300), a degassing box (EG degasser), and a capillary cation ion chromatography column (Cation exchange Capillary column). Triple quadrupole mass spectrometer (AB Company).

[0064] system connection as Figure 10 Shown: The pump of the capillary ion chromatograph is connected to the acid eluent bottle, the eluent bottle is connected to the degassing box, the degassing box is connected to the switching valve, the quantitative loop is connected to the swi...

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Abstract

The invention relates to a system with the combination of capillary tube ion chromatography and mass spectrum, to solve the problems that mobile phases are not compatible when the ion chromatography is combined with the mass spectrum and the sensitivity and the separation degree in analysis and detection of certain polar compounds are low. By additionally arranging a cation membrane electrolysis device or an anionic membrane electrolysis device between the capillary tube ion chromatography and the mass spectrum, acid or alkali leacheate from an ion chromatograph is converted into water so as to be enabled to enter a mass spectrometer directly. A quantitative ring or a capture column, a capillary-column chromatography, an electrolytic ion exchange device and the mass spectrum are all connected by using capillary tubes; and the inner diameters of the capillary tubes are 0.05-0.1mm. By utilizing the system with the combination of the ion chromatography and the mass spectrum, problems of incompatibility of the mobile phases and salt interference in the combination of the ion chromatography and the mass spectrum are effectively solved, high selectance and sensitivity to the polar compounds are achieved, and moreover, isomerides can be separated efficiently; and the system has simple equipment, and is simple to operate, less in necessary sample quantity and easy in industrialized application.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, and can be widely used in the fields of biological energy research, life science, food science, environment, petrochemical industry and the like. Background technique [0002] In the analysis of a large number of water-soluble components in complex systems such as biological samples, ionic and polar compounds such as organic acids, carbohydrates, nucleic acids and amino acids use traditional liquid chromatography-mass spectrometry (LC-MS) methods. Separation is difficult and the separation effect is poor. Some ionic and polar compounds such as organic acids, carbohydrates, nucleic acids and amino acids are difficult to separate on traditional reversed-phase columns and even remain on the column. The separation effect of ion chromatography is better than that of LC-MS, but the effective identification of such polar metabolites is limited by the existing detectors only using retention...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/60G01N30/62
Inventor 法芸高峻杨孟龙白罚利杜鹃郑岳观文娜杨海燕
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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