Leukemia single-chain antibody library, as well as construction method and application thereof

A single-chain antibody, leukemia technology, applied in the field of genetic engineering, can solve the problems of immunogenicity, problems, limitations, etc., and achieve the effect of large library capacity, good diversity, and strong operability
CN102978713BActive Publication Date: 2015-02-25ZHEJIANG UNIV
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2015-02-25

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a leukemia bacteriophage single-chain antibody library, which uses phasmid pCANTA-5E as a carrier, has storage capacity of 1.5*10<9>, and has excellent diversity in proving of DNA (deoxyribonucleic acid) sequencing. A leukemia cell specific completely humanized single-chain antibody can be obtained conveniently in multiple screenings by using a specific leukemia related antigen as a target through a bacteriophage representation technology from the completely humanized single-chain antibody library. The antibody library provided by the invention has high storage capacity and excellent diversity, and comprises a combination of complete humanized single-chain antibodies; and a single-chain antibody is formed by connection of a heavy chain variable region VH and a light chain variable region VL through a connecting peptide (GGGGS)3, and contains a complete antigen-binding site. The method has the advantages of strong operability and high storage capacity, and can be used for conveniently screening the leukemia cell specific completely humanized single-chain antibody; and the completely humanized single-chain antibody can be used for preparing a target medicament, which is used for treating leukemia.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to genetic engineering, and relates to a fully human leukemia single-chain antibody library and its construction method, and its application in the preparation of leukemia antibody medicine. Background technique

[0002] since Since he and Milstein founded lymphocyte hybridoma technology, monoclonal antibodies have been widely used in the field of tumor diagnosis and treatment. The first generation of mouse monoclonal antibody used in the treatment of human diseases is mainly mouse-derived monoclonal antibody, but the main disadvantage of mouse monoclonal antibody is that it causes the human immune response, and the human anti-mouse antibody produced by the human body makes it clear quickly and cannot play its biological role well Therefore, the clinical application is limited; the second-generation antibody is based on the mouse monoclonal antibody and forms a chimeric antibody and a humanized antibody through gene recombinatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More