Detection method of thymol content

A detection method and a technology for thymol are applied in the detection field of thymol content and achieve the effect of being conducive to popularization and application.

Inactive Publication Date: 2013-03-20
谱尼测试集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No detection method for thymol in detergents and cosmetics has been found in the prior art literature. Thymol is used in detergents and cosmetics to have a certain limit requirement, so an accurate

Method used

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  • Detection method of thymol content

Examples

Experimental program
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Effect test

Example Embodiment

[0019] Example: Detection of thymol in soap.

[0020] 1. Sample processing

[0021] Weigh 0.2g (accurate to 0.0001g) soap sample into a 10mL volumetric flask, add 8mL methanol, ultrasonically extract for 30min, then cool to room temperature, add methanol to the volume and shake up. The sampled solution was passed through a 0.45μm filter membrane and then quantitatively analyzed by high performance liquid chromatography.

[0022] 2. The analysis conditions of HPLC are:

[0023] Chromatographic column: use carbon octadecyl bonded silica as filler;

[0024] Mobile phase: methanol:water=62:38;

[0025] Column temperature: 30℃;

[0026] Flow rate: 1.0mL / min;

[0027] Injection volume: 10μL;

[0028] Wavelength: 276nm;

[0029] 3. Configuration of standard solution and drawing of standard curve

[0030] 3.1 Configuration of standard stock solution: Weigh 10 mg of thymol standard into a 10 mL volumetric flask, add 8 mL of methanol to dissolve ultrasonically, then cool to room temperature, add metha...

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Abstract

The invention discloses a detection method of thymol content. According to the invention, thymol in a sample is extracted by using an organic reagent; the sample is subjected to ultrasonic sufficient extraction, and volume metering is carried out; an extraction liquid is subjected to high-performance liquid chromatography system determination, wherein chromatographic column separation is carried out with carbon octadecyl bonded silica as a filling agent, a mobile phase is a mixed solvent of methanol and water wherein methanol accounts for 60-70% and water accounts for 40-30%, and a detection wavelength is 276nm. The invention establishes a detection method of thymol content in detergents and cosmetics. The method has the technical characteristics of good separation effect, accurate determination, high sensitivity, high specificity, convenient and rapid analysis, and the like.

Description

technical field [0001] The invention relates to a detection method of thymol content, in particular, the invention relates to a detection method of thymol content in detergents and cosmetics. Background technique [0002] Thymol (Thymol), also known as thymol, because the bactericidal effect of thymol is stronger than that of phenol, and its toxicity is low, it can be used as a preservative in controlling bee mites, preserving books, and anesthetics; at the same time as a spice, musk Grass phenol is a food spice that is allowed to be used in my country's "Hygienic Standards for the Use of Food Additives". It is mainly used to prepare flavors such as cough syrup, peppermint chewing gum, and spices. It can also be used as toothpaste, soap, and some cosmetic flavor formulations. [0003] At present, the methods for determining thymol mainly include ultraviolet spectrophotometry, gas chromatography and high performance liquid chromatography, and the detection field is mainly drug...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 宋薇
Owner 谱尼测试集团股份有限公司
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