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Agent for suppressing the formation of abnormal skin cells caused by exposure to light

一种皮肤细胞、异常的技术，应用在皮肤疾病、非有效成分的医用配制品、含有效成分的医用配制品等方向

Inactive Publication Date: 2013-03-20
OTSUKA PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] As mentioned above, no studies have been conducted on the relationship between photocarcinogenesis and (skin) topical application of purine nucleic acids, how purine nucleic acids, when applied topically, affect the suppression of abnormal skin cell formation caused by light exposure, or the prevention of photocarcinogenesis or treatment is completely unknown

Method used

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  • Agent for suppressing the formation of abnormal skin cells caused by exposure to light
  • Agent for suppressing the formation of abnormal skin cells caused by exposure to light
  • Agent for suppressing the formation of abnormal skin cells caused by exposure to light

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: Effect of Inducing Apoptosis in Mutant Cells Caused by Ultraviolet Light Irradiation

[0075] In this example, changes in caspase activity were detected when human epithelial cells treated with 5'-adenosine monophosphate disodium (AMP2Na) were irradiated with ultraviolet rays.

[0076]

[0077]Using EpiLife-KG2 medium (manufactured by Kurabo Industries Ltd.), human epithelial keratinocytes (purchased from Kurabo Industries Ltd. ) and seeded at a density of 30,000 cells / well into collagen-coated 96-well microplates. After culturing in EpiLIfe-KG2 medium for 8 hours, the medium was replaced with EpiLife medium (manufactured by Kurabo Industries Ltd.), followed by further culturing for 16 hours. Subsequently, the medium was replaced with AMP2Na-containing medium adjusted to various concentrations. After being treated with the culture medium for 2 hours, the cells were washed with PBS (phosphate buffered saline) at 30 mJ / cm using an ultraviolet light irradi...

Embodiment 2

[0082] Example 2: Evaluation of the Effect on Reducing DNA Mutations Induced by UV Light Irradiation

[0083] In this example, normal mouse epithelial cells were irradiated with ultraviolet rays and then cultured in a medium containing AMP2Na. The effect of AMP2Na on reducing DNA mutations was examined using the amount of cyclobutane pyrimidine dimer (CPD) as an index.

[0084]

[0085] JB6 cells (purchased from ATCC) derived from normal mouse epithelial cells were cultured in FBS-containing MEM medium until confluent. Subsequently, the 4×10 5 The cultured cells were seeded into a 3.5 cm dish with MEM (minimum essential medium) containing FBS (fetal bovine serum). The day after inoculation, the medium was replaced by serum-free MEM medium for serum starvation. Subsequently, the medium was replaced by PBS, followed by 15 mJ / cm 2 of UV-B irradiation. After UV irradiation, the medium was replaced with serum-free MEM medium containing 0.01 mM, 0.1 mM or 1 mM AMP2Na or med...

Embodiment 3

[0091] Example 3: Evaluation of Preventive Effects on Skin Cancer in Ultraviolet-Irradiated Mice

[0092] In this example, hairless mice were irradiated with ultraviolet rays, and the preventive effect of AMP2Na against photocarcinogenesis in mice was tested.

[0093]

[0094] 5-week-old female Hos:HR-1 mice were purchased from Japan SLC, Inc., and their backs were irradiated with ultraviolet (UV--B) from 7 weeks of age. Ultraviolet light irradiation (each irradiation dose: 60mJ / cm 2 ) once a day, five days a week for 12 weeks.

[0095] Then, a 20% ethanol aqueous solution (test solution) containing 3% AMP2Na dissolved therein and a 20% ethanol aqueous solution (substrate) not containing AMP2Na were prepared.

[0096] After feeding the ultraviolet-irradiated mice under normal conditions for 1 week, the mice were divided into 3 groups (6 mice per group) under the conditions shown in Table 1 and fed for 12 weeks.

[0097] Table 1

[0098]

[0099]

[0100] The condi...

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PUM

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Abstract

An object of the present invention is to provide an agent for suppressing the formation of skin cells induced by exposure to light such as ultraviolet light. The object is achieved by using a purine nucleic acid for suppressing the formation of abnormal skin cells caused by exposure to light.

Description

technical field [0001] The present invention relates to agents for suppressing the formation of abnormal skin cells caused by light exposure. Background technique [0002] In recent years, the effect of light exposure such as sun exposure on the skin is becoming a serious world problem. In particular, an increase in the incidence of skin cancer has become a serious problem in the United States, Europe, and Australia. One of the factors contributing to the increase in skin cancer is the increasing amount of ultraviolet light exposure in daily life due to the destruction of the ozone layer by chlorofluorocarbons. In today's world, it is impossible to avoid the risk of abnormal skin cell formation caused by light exposure, especially the risk of photocarcinogenesis (ie skin cancer induced by light exposure such as UV exposure). [0003] Skin cancer is traditionally treated with a combination of surgery, chemotherapy, and radiation. However, depending on when skin cancer is d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7076A61P17/16A61P35/00
CPCA61K47/10A61K9/00A61K9/0014A61K31/7076A61P17/00A61P17/16A61P35/00A61P43/00
Inventor 河村光章筱原茂生原野史树青木彰宽上野绘理
Owner OTSUKA PHARM CO LTD
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