Hexaconazole copper complex and application therof
A technology of copper complexes and hexaconazole, applied in copper organic compounds, applications, pest control, etc., can solve the problems of bactericidal mechanism and single action site, loss of high bactericidal activity, etc.
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Embodiment 1
[0022] Example 1 Preparation and Characterization of Hexaconazole Copper Complex
[0023] 1. Preparation of complexes
[0024] Dissolve 0.0354 g (0.11 mmol) hexaconazole in 8 mL absolute ethanol, 0.010 g (0.05 mmol) Cu(OAc) 2 ·H 2 O was dissolved in 4 mL of aqueous solution, and the hexaconazole absolute ethanol solution was dropped into Cu(OAc) 2 ·H 2 In O aqueous solution, the mixed solution was stirred at room temperature for 1 h to obtain a light blue clear solution, which was filtered after the reaction, and the filtrate was left standing at room temperature, and blue transparent needle-like crystals were precipitated after two weeks.
[0025] 2. Complex characterization
[0026] The elemental analysis was carried out by the VarioEL III elemental analyzer from Ellerman Elemental Analysis System Company in Germany; the infrared spectrum analysis was carried out by EQUINOX-55 from Bruker, Germany; the thermogravimetric analysis was carried out by STA 449C, a German NETZ...
Embodiment 2
[0037] Example 2 Bacteriostatic activity test of hexaconazole copper complex
[0038] 1. Experimental design
[0039] The structural analysis of the hexaconazole copper complex showed that two hexaconazoles were coordinated with one copper ion. Therefore, this study compared the hexaconazole copper complex with the hexaconazole at a concentration of 2 times; 2+ As heavy metal ions, it may affect the growth of the tested yeast, so sterile water and 1-fold concentration of copper acetate solution were used as experimental controls.
[0040] Both hexaconazole copper complex and hexaconazole are insoluble in water. When preparing the solution, first dissolve it with a small amount of DMF, and then add sterile water to dilute to the test concentration.
[0041] 2. Test method
[0042] Pick a single clone of Saccharomyces cerevisiae and inoculate it in 20 mL of YPD liquid medium for 24 h at 28°C in a shaker flask at 200 rpm to activate the strain; measure the optical density of t...
Embodiment 3
[0048] Example 3 Hexaconazole Copper Complex Regulates Plant Growth
[0049] Hexaconazole has a significant effect on the defense and treatment of diseases suffered by cereal crops, and the rational use of hexaconazole can guarantee the value maintenance and income increase of crops. Studies have shown that hexaconazole can effectively reduce the damage caused by salt stress to the growth of rape seedlings by promoting root growth, increasing dry weight, increasing chlorophyll, soluble protein content, and increasing antioxidant enzyme activity.
[0050] 1. Materials and methods
[0051] Wheat species (Shaanong 138); hexaconazole copper complex; hexaconazole.
[0052] 2. Experimental design
[0053] Structural analysis of hexaconazole copper complex shows that two hexaconazoles and one Cu 2+ Coordination, therefore, this experiment compares hexaconazole copper complexes with 2 times the concentration of hexaconazole; Cu 2+ As heavy metal ions may affect the growth of the tes...
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