Culture technique for aseptic pepper seedlings

Abstract

The invention discloses a culture technique for aseptic pepper seedlings, which belongs to the field of plant biotechnology. The technique is mainly implemented through a way that an aseptic water vibrated seed-soaking process is added before a culture medium inoculation process in the processes (tap water cleaning-super clean bench disinfection-ethanol disinfection-mercury chloride disinfection-aseptic water cleaning-culture medium inoculation) of the existing culture technique for aseptic pepper seedlings, so that six processes are changed into seven processes (tap water cleaning-super clean bench disinfection-ethanol disinfection-mercury chloride disinfection-aseptic water cleaning-aseptic water vibrated seed-soaking-culture medium inoculation). The technique can carry out detoxification, oxygenation, budding promotion and growth promotion on pepper seeds, and has the advantages of simple operation, rapid bud seedling growth, high stocking percent, and tidy and robust seedling bodies.

Description

technical field [0001] A method for cultivating aseptic pepper seedlings belongs to the field of biotechnology, more specifically, on the basis of the existing technology for cultivating sterile pepper seedlings, a process of oscillating and soaking seeds in sterile water is added to detoxify and oxygenate, and promote Sprout growth, a method for improving the efficiency of aseptic seedling cultivation. Background technique [0002] The acquisition of sterile explants is an important part of plant tissue culture, which can be used in protoplast fusion, cell culture, in vitro rapid propagation and plant transgenic. The aseptic explants of dicotyledonous plants are mostly obtained by sterilizing the seeds and transforming them into aseptic seedlings, then taking their leaves and hypocotyls for in vitro rapid propagation or transgenic research. Therefore, the culture cycle of aseptic seedlings And the orderliness of the group became the bottleneck restricting the follow-up exp...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the growth of sterile seedlings is slow, the seedling time is long, and the efficiency is low.

After analysis, we believe that the reason for the poor growth of sterile seedlings in the above method is that after mercuric chloride disinfection, the residual mercuric chloride on the surface or inside of the seeds is poisonous to the seeds and affects the viability of the seeds; When inoculated on agar medium, due to different inoculation depths and different body positions, the water absorption of the seeds is slow and not uniform, resulting in slow emergence and different sizes, and low work efficiency