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Synthetic method of magnetic graphene mesoporous material used for separation and enrichment in peptideomics

A technology of separation, enrichment and synthesis method, which is applied in the field of synthesis of magnetic graphene mesoporous materials, can solve the problems of small surface area and limited functional groups, and achieves the effect of maintaining biological activity, being beneficial to adsorption, and having abundant surface functional groups.

Inactive Publication Date: 2014-12-03
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the separation and analysis of peptide / proteomics, the current separation and analysis materials commonly used in the world mainly include nano-microspheres, carbon nanotubes, etc., but these materials have small surface areas and limited functional groups that can be modified, which in a sense restricts In peptide / proteomics, the application of specific separation and enrichment of related peptides / proteins

Method used

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  • Synthetic method of magnetic graphene mesoporous material used for separation and enrichment in peptideomics
  • Synthetic method of magnetic graphene mesoporous material used for separation and enrichment in peptideomics
  • Synthetic method of magnetic graphene mesoporous material used for separation and enrichment in peptideomics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Disperse graphene in concentrated nitric acid, mechanically stir at 60°C for 6 hours, add sodium hydroxide solution dropwise until it becomes moderately acidic, and dry it.

[0027] (2) 800mg FeCl 3 ·6H 2 Dissolve O in ethylene glycol, add 0.15g of sodium citrate and 150mg of acidified graphene obtained in step (1), and ultrasonically disperse for two hours, then add 3.5g of sodium acetate and 2g of PEG-20000, and heat in a reactor at 200°C High pressure reaction for 8-12 hours.

[0028] (3) Disperse 50mg of hydrophilic magnetic graphene and 500mg of cetyltrimethylammonium bromide surfactant obtained in step (2) into 50ml of deionized water and ultrasonicate for half an hour, add 400ml of deionized water and 50ml 0.01M sodium hydroxide solution, after ultrasonication for 10 minutes, add 2.5ml ethyl orthosilicate ethanol solution (volume ratio of ethyl orthosilicate: ethanol is 1:4), mechanically stir at 60°C for 12 hours, wash and dry.

[0029] (4) Wash off the c...

Embodiment 2

[0033] (1) Disperse graphene in concentrated nitric acid, mechanically stir at 60°C for 6 hours, add sodium hydroxide solution dropwise until it becomes moderately acidic, and dry it.

[0034] (2) Add 200mg FeCl 3 ·6H 2 Dissolve O in ethylene glycol, add 0.15g of sodium citrate and 150mg of acidified graphene obtained in step (1), and ultrasonically disperse for two hours, then add 3.5g of sodium acetate and 2g of PEG-20000, and heat it in a reactor at 200°C High pressure reaction for 8-12 hours.

[0035](3) Disperse 50mg of hydrophilic magnetic graphene and 500mg of sodium dodecylbenzenesulfonate surfactant obtained in step (2) into 50ml of deionized water and ultrasonicate for half an hour, add 400ml of deionized water and 50ml of 0.01M Sodium hydroxide solution, after ultrasonication for 10 minutes, add 2.5ml ethyl orthosilicate ethanol solution (volume ratio of ethyl orthosilicate: ethanol is 1:4), mechanically stir at 60°C for 12 hours, wash and dry.

[0036] (4) Wash ...

Embodiment 3

[0039] (1) Disperse graphene in concentrated nitric acid, mechanically stir at 60°C for 6 hours, add sodium hydroxide solution dropwise until it becomes moderately acidic, and dry it.

[0040] (2) Add 600mg FeCl 3 ·6H 2 Dissolve O in ethylene glycol, add 0.15g of sodium citrate and 150mg of acidified graphene obtained in step (1), and ultrasonically disperse for two hours, then add 3.5g of sodium acetate and 2g of PEG-20000, and heat in a reactor at 200°C High pressure reaction for 8-12 hours.

[0041] (3) Disperse 50mg of hydrophilic magnetic graphene and 500mg of stearic acid surfactant obtained in step (2) into 50ml of deionized water and ultrasonicate for half an hour, add 400ml of deionized water and 50ml of 0.01M sodium hydroxide solution, After ultrasonication for 10 minutes, add 2.5ml ethyl orthosilicate ethanol solution (volume ratio of ethyl orthosilicate: ethanol is 1:4), mechanically stir at 60°C for 12 hours, wash and dry.

[0042] (4) Wash away the stearic aci...

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Abstract

The invention belongs to the field of advanced nanopore adsorbent materials and nanotechnology, and specifically relates to a synthetic method of a magnetic graphene mesoporous material used for separation and enrichment in peptideomics. The synthetic method comprises the steps of acidifying the graphene, dispersing the acidified graphene in glycol containing an iron source, forming the magnetic graphene at a high temperature and a high pressure, then dispersing the obtained material in an ethanol / water solution containing a silicon source and a surfactant to make the silicon source pre-hydrolyzed and subjected to relevant reactions with the magnetic grapheme, further performing refluxing treatment on the reacted product in a solvent, removing polymer microsphere templates and the surfactant via extraction, and finally carrying out high-temperature calcinations. The method is simple, has easily available raw materials and is suitable for amplification production. With the method, the magnetic graphene material having a mesoporous structure and a hydrophobic surface can be obtained. The pore wall and the surface of the material are hydrophobic surfaces, and the material can be used for separation and enrichment of endogenous peptide segments in peptideomics. Besides, the material has no biological toxicity, so that the material can be widely applied on biomedical platforms.

Description

technical field [0001] The invention belongs to the field of advanced nanoporous adsorbent materials and nanotechnology, and specifically relates to a synthesis method of a magnetic graphene mesoporous material used for separation and enrichment of peptidomics. Background technique [0002] With the development of modern technology, people have a deeper understanding of the importance of peptidomics in human health and disease diagnosis. According to literature reports, peptides play a central role in biological processes, especially some hormones, cytokines, and growth factors have been known and studied for many years, and nanomaterials have been widely used as materials for the separation and analysis of peptide / proteomics. acceptance and application. [0003] In recent years, the research on graphene materials has become more and more mature. Due to its excellent mechanical, thermal, electrical and magnetic properties, it is expected to be used in high-performance nanoe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/20B01J20/30B01J20/28C07K1/22
Inventor 邓春晖尹鹏孙念荣赵曼熊娅王梦依闫迎华
Owner FUDAN UNIV
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