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2,3-butanediol high-producing strain, and screening method and fermentation method thereof

A high-yielding strain, butanediol technology, applied in the biological field, can solve the problems of difficult chemical method, difficult industrial production, difficult operation, etc., and achieves the effects of improving screening efficiency, reducing workload and increasing cost

Inactive Publication Date: 2013-10-30
TIANJIN UNIV OF SCI & TECH
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  • Description
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Problems solved by technology

[0004] The chemical synthesis of 2, 3-butanediol is obtained by hydrolysis of four-carbon hydrocarbons produced during petroleum cracking, but this method is not easy to operate, and due to the shortage of petroleum resources and the increase in the price of petrochemical products, It is difficult to realize industrial production, and microbial production of 2, 3-butanediol has overcome the difficulties of chemical methods and has attracted people's attention

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  • 2,3-butanediol high-producing strain, and screening method and fermentation method thereof
  • 2,3-butanediol high-producing strain, and screening method and fermentation method thereof
  • 2,3-butanediol high-producing strain, and screening method and fermentation method thereof

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Embodiment Construction

[0033] The present invention is further described below in conjunction with embodiment; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0034] The reagents used in this example are all commercially available products unless otherwise specified; the methods used are all conventional methods unless otherwise specified; the instruments used are all conventional instruments unless otherwise specified.

[0035] A 2, 3-butanediol high-yielding strain named Serratia marcescens G12, classified as Serratia marcescens, deposit number: CGMCC No. 6770, deposit date: November 02, 2012, deposit Address: Datun Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, preservation unit: General Microbiology Center of China Committee for the Collection of Microbial Cultures.

[0036] The present invention isolated a 2, 3-butanediol-producing strain #12 from unprocessed milk,...

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Abstract

The invention relates to a 2,3-butanediol high-producing strain which is characterized in that the name of the strain is Serratia marcescens G12, the systematic name is Serratia marcescens, the collection number is CGMCC No.6770, the collection date is November 2nd, 2012, the collection address is Institute of Microbiology, Chinese Academy of Sciences at Datun Road, Chaoyang District of Beijing, and the collection unit is China General Microbiological Culture Collection Center. The 2,3-butanediol high-producing strain disclosed by the invention is an ideal candidate strain for producing 2,3-butanediol; and after the 2,3-butanediol high-producing strain is fermented, the yield of 2,3-butanediol reaches 37.4 g / L, and the substrate conversion rate accounts for 79.62% of a theoretic conversion rate.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a 2, 3-butanediol producing strain, especially a 2, 3-butanediol high-yielding strain, a screening method and a fermentation method thereof. Background technique [0002] 2, 3-butanediol (2, 3-butanediol, 2, 3-BD), also known as dimethylmethanol, 2, 3-butanediol is a colorless and odorless liquid with strong water absorption and melting point The temperature is 23-27°C, the boiling point is 178-182°C, and the freezing point is -60°C. It can be miscible with water and soluble in ethanol and ether. [0003] 2, 3-Butanediol can be used as an important chemical raw material and pharmaceutical intermediate. It has a lower freezing point and can be used as antifreeze, 2, 3-butanediol and its derivatives can be used as plastics, solvents and drug carriers; dehydrogenation can form diacetyl and acetoin, which are used as flavor enhancers in daily life Elixirs, margarines and cosmetics. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/18C12Q1/04C12R1/43
Inventor 杨洪江高松松
Owner TIANJIN UNIV OF SCI & TECH
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