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Method for producing 2,3-butanediol by use of safe strain-fermented biodiesel byproduct crude glycerin

A technology for fermenting organisms and biodiesel, which is applied in the direction of fermentation, microorganisms, and methods based on microorganisms. It can solve problems that do not meet industrial safety production, achieve high industrial application value, and reduce production costs.

Active Publication Date: 2014-07-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the currently reported 2,3-butanediol producing strains are Klebsiella (Klebsiella), Enterobacter (Enterobacter) and Serratia (Serratia), etc., but most of these bacteria are produced by fermentation of sugary raw materials 2,3-butanediol, but domestic production of 2,3-butanediol using crude glycerol as a raw material has not been reported; in addition, these strains have potential pathogenicity and do not meet the requirements of industrialized safe production

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  • Method for producing 2,3-butanediol by use of safe strain-fermented biodiesel byproduct crude glycerin

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Embodiment 1

[0018] (1) B. subtilis CTCC M2009200 seed culture

[0019] Pick a single colony from the activated plate and inoculate it in the seed medium for seed culture. The culture temperature is 37°C, the shaker speed is 160r / min, and the culture time is about 12h. The composition of the seed medium: yeast extract 5g / L, tryptone 10g / L, NaCl 10g / L;

[0020] (2) B. subtilis CTCC M2009200 fermentation culture

[0021] The initial fermentation culture volume is 2.5L, and the components of the fermentation medium used are as follows:

[0022] Crude glycerin 80g / L, corn steep liquor 10g / L, urea 3g / L, sodium citrate 6g / L, K 2 HPO 4 4g / L, MgSO 4 0.2g / L; adjust the pH of the above fermentation medium to 6.5 with 5mol / L NaOH, and sterilize at 121°C for 30min.

[0023] Crude glycerin aqueous feed solution: 500 / 600mL;

[0024] Inoculate the above-mentioned cultivated seed liquid into the fermentation medium according to the inoculum amount of 4% to carry out fermentation culture, the ferme...

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Abstract

The invention discloses a method for producing 2,3-butanediol by use of environmental-safety strain bacillus subtilis CTCC M2009200-fermented biodiesel byproduct crude glycerin, belonging to the field of fermentation technology in bioengineering. The method mainly comprises seed culture and fermentation culture, and specifically comprises the following steps of: inoculating the seed liquid in a fermentation culture medium according to an inoculation amount of 4%; performing fermentation culture in the culture medium with air flow of 120m<3> / h.m<3> at 37 DEG C and at a stirring speed of 350r / min; when the glycerin concentration in the fermentation liquid is lower than 15g / L, adding crude glycerin supplement liquid in a flow manner so as to maintain the concentration of glycerin in the culture liquid at 0-20g / L; and when the consumption rate of glycerin is lower than 1g / l.h, stopping the fermentation immediately, wherein the fermentation liquid is liquid containing 2,3-butanediol. The method disclosed by the invention lays a foundation for the industrial deep development of the biodiesel byproduct crude glycerin.

Description

technical field [0001] The invention relates to producing 2,3-butanediol by fermenting biodiesel by-product crude glycerol with a safe bacterial strain, which belongs to the technical field of fermentation in bioengineering. Background technique [0002] With the increasing shortage of energy resources and the increasingly serious environmental problems, the development of renewable biomass resources as raw materials, the biorefining technology of producing various chemicals, functional materials and energy substances through chemical, biological and integrated methods is becoming more and more important. more and more attention at home and abroad. As a promising biological resource, biodiesel has attracted attention from all over the world because of its environmental protection and renewability, and the industry is developing rapidly around the world. But its economy still cannot compete with traditional fossil energy. There are two main reasons for this: on the one hand...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12R1/125
Inventor 饶志明杨套伟张显徐美娟满在伟
Owner JIANGNAN UNIV